Dental pulp stem cell resuscitation fluid and dental pulp stem cell resuscitation method
A technology of dental pulp stem cells and resuscitation fluid, applied in the field of stem cell culture, can solve problems such as invasive injury of donors, save time, maintain cell stemness and multi-directional differentiation potential
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[0025] The present invention will be described in detail below in conjunction with examples, which are only preferred implementations of the present invention, and are not limitations of the present invention.
[0026] Preparation of Dental Pulp Stem Cell Recovery Solution:
[0027] It is mainly composed of conventional medium for mesenchymal stem cells and basic fibroblast growth factor (bFGF), wherein the conventional cell culture medium is: a-MEM medium (Gibco, USA) + 10% fetal bovine serum (FBS, Gibco, USA)+100U / mL penicillin+100μg / mL streptomycin (Gibco, USA). The final concentrations of bFGF were 5ng / mL, 10ng / mL, 20ng / mL, 50ng / mL, 80ng / mL and 100ng / mL.
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