Dental pulp stem cell resuscitation fluid and dental pulp stem cell resuscitation method
A technology of dental pulp stem cells and resuscitation fluid, applied in the field of stem cell culture, can solve problems such as invasive injury of donors, save time, maintain cell stemness and multi-directional differentiation potential
Active Publication Date: 2017-10-20
浙江优牙生物科技有限公司 +2
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The source of stem cells is mainly embryonic stem cells and mesenchymal stem cells, among which embryonic stem cells are limited due to the ethical issues involved in their clinical translation research; the main source of mesenchymal stem cells is bone marrow, that is, bone marrow mesenchymal stem cells, but in the process of clinical translation research There are disadvantages such as invasive damage to the donor
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[0025] The present invention will be described in detail below in conjunction with examples, which are only preferred implementations of the present invention, and are not limitations of the present invention.
[0026] Preparation of Dental Pulp Stem Cell Recovery Solution:
[0027] It is mainly composed of conventional medium for mesenchymal stem cells and basic fibroblast growth factor (bFGF), wherein the conventional cell culture medium is: a-MEM medium (Gibco, USA) + 10% fetal bovine serum (FBS, Gibco, USA)+100U / mL penicillin+100μg / mL streptomycin (Gibco, USA). The final concentrations of bFGF were 5ng / mL, 10ng / mL, 20ng / mL, 50ng / mL, 80ng / mL and 100ng / mL.
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A dental pulp stem cell resuscitation fluid and a dental pulp stem cell resuscitation method are disclosed, the new stem cell resuscitation fluid is constructed by addition of a certain amount of basic fibroblast growth factors into a conventional stem cell culture medium, the new stem cell resuscitation fluid is used in resuscitation process of DPSCs (dental pulp stem cells) for rapid recovery of activity of the DPSCs in a short period of time, stemness and multiple differentiation potential of the original stem cells can be maintained, the resuscitation time of the stem cells can be shortened, the clinical use survival rate of the stem cells can be improved, the proliferation rate exceed that of a conventional medium group, meanwhile the cell stemness and multiple differentiation potential can be maintained, seed cells with better performance and status can be effectively provided for clinical stem cell therapy, and a lot of time, manpower and material cost can be saved.
Description
technical field [0001] The invention specifically relates to the technical field of stem cell culture, in particular to a dental pulp stem cell resuscitation solution and a dental pulp stem cell resuscitation method. Background technique [0002] Stem cells are a type of cells with self-renewal, high proliferation and multi-directional differentiation capabilities, which can be induced to differentiate into mature human tissues and organs under specific conditions, and have been widely used in various aspects of regenerative medicine research. The source of stem cells is mainly embryonic stem cells and mesenchymal stem cells, among which embryonic stem cells are limited due to the ethical issues involved in their clinical translation research; the main source of mesenchymal stem cells is bone marrow, that is, bone marrow mesenchymal stem cells, but in the process of clinical translation research There are disadvantages such as invasive damage to the donor. Since Gronthos et...
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IPC IPC(8): C12N5/0775A01N1/02
CPCA01N1/0221C12N5/0664C12N2501/115C12N2509/00
Inventor 叶青松罗丽花王晓燕郑丽娜
Owner 浙江优牙生物科技有限公司
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