Culture medium and application thereof as well as method for culturing dental pulp stem cells
A medium and quality stem cell technology, applied in the field of stem cell culture, can solve problems such as difficulties, contamination and purification of cells, and achieve the effects of short cell cycle, good cell uniformity, and ensuring purity and dryness.
Inactive Publication Date: 2017-02-01
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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Problems solved by technology
[0004] Stem cells have broad application prospects in regenerative medicine and cell therapy, drug screening and early embryonic development research, but their research still faces huge challenges, the most critical of which are stem cell culture reagents, which are usually required in the traditional stem cell culture process. Adding a certain amount of fetal bovine serum, which contains growth factors, hormones, carrier proteins, adhesion factors, trace elements and other nutrients required for cell growth, can promote cell growth, but the medium containing serum is contaminated with pathogens Causes the problem of difficult purification of cells, and can improve the stability of cell production, increase the amount of cell proliferation, and enhance cell viability
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Embodiment 1
[0049] Embodiment 1 The cultivation of dental pulp mesenchymal stem cells
[0050] The formula of the culture medium of each test group is shown in Table 1:
[0051] Table 1 The formulation of each component differentiation induction solution
[0052] Experimental group 1 Experimental group 2 Experimental group 3 Control group 1 Control group 2 serum replacement 2％ 15％ 7.5％ 7.5％ 7.5％ EGF 0.5ng / ml 10ng / ml 5ng / ml 5ng / ml -- FGF 0.5ng / ml 10ng / ml 5ng / ml 5ng / ml -- PDGF 10ng / ml 0.5ng / ml 5ng / ml 5ng / ml -- VEGF 10ng / ml 0.5ng / ml 5ng / ml 5ng / ml -- TGF 10ng / ml 0.5ng / ml 5ng / ml 5ng / ml -- L-Glutamine 1mmol / mL 5mmol / mL 3mmol / mL 3mmol / mL 3mmol / mL albumin 250pg / ml 250pg / ml 250pg / ml 250pg / ml 250pg / ml insulin 1μg / mL 100μg / mL 50μg / mL 50μg / mL 10μg / mL Soy Isoflavones 1mg / L 0.5mg / L 0.1mg / L -- 0.5mg / L
[0053] Cut the pulp tissue into pieces, ...
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Abstract
The invention relates to the technical field of stem cell culture and in particular relates to a culture medium and application thereof as well as a method for culturing dental pulp stem cells. According to the culture medium, a serum replacement, an EGF (Epidermal Growth Factor), an FGF (Fibroblast Growth Factor), a PDGF (Platelet Derived Growth Factor), a VEGF (Vascular Endothelial Growth Factor), L-glutamine, albumin, insulin and soybean isoflavone are added into a serum-free culture medium; each factor and the soybean isoflavone have a good promotion effect on growth of the dental pulp stem cells. An experiment shows that a cell period can be shortened when the culture medium is used for culturing dental pulp mesenchymal stem cells, the cell proliferation ability is improved, and the purity and dryness of the stem cells are improved; the culture medium does not contain animal derived ingredients including fetal bovine serum and the like, and the stem cells obtained by the culture medium is suitable for clinical application.
Description
technical field [0001] The invention relates to the technical field of stem cell culture, in particular to a culture medium, its application and a method for culturing dental pulp stem cells. Background technique [0002] Human dental pulp mesenchymal stem cells (hDPMSCs) have the advantages of extensive sources, strong plasticity, no ethical debates, and strong potential to differentiate into multiple tissues. Therefore, hDPMSCs have great potential in research fields such as tissue engineering and gene therapy. The potential seed cells have very broad prospects in clinical application. [0003] In addition to the therapeutic potential of plastic surgery and maxillofacial repair, DPSCs also have therapeutic effects on many other diseases. Most studies to date have shown that dental pulp stem cells derived from third molars and deciduous teeth can give rise to mineralized tissues, dentin-like extracellular matrix and structures, periodontal ligaments and pulp, among other s...
Claims
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IPC IPC(8): C12N5/0775
CPCC12N5/0664C12N2500/32C12N2500/90C12N2501/11C12N2501/113C12N2501/115C12N2501/117C12N2501/119C12N2501/135C12N2501/165C12N2501/30C12N2501/33C12N2501/998
Inventor 王一飞陈海佳葛啸虎刘帅王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD