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Preparation method of 6-aminopenicillanic acid

A technology of aminopenicillin and penicillin, which is applied in the field of preparation of 6-aminopenicillin, can solve problems such as low effective dose of penicillin, and achieve the effects of solving low penicillin content and improving conversion efficiency.

Active Publication Date: 2021-06-22
JINING TECHNICIAN COLLEGE
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Problems solved by technology

[0009] In order to solve the above problems and overcome the deficiencies in the prior art, the present invention provides a preparation of 6-aminopenicillanic acid, which can effectively solve the problem of low effective dosage of penicillin

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  • Preparation method of 6-aminopenicillanic acid

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specific Embodiment approach

[0034] A method for preparing 6-aminopenicillanic acid, using the penicillin fermentation liquid to obtain the treated penicillin fermentation liquid through a pretreatment process;

[0035] The penicillin fermented liquid is obtained by fermentation of Penicillium chrysogenum, and Penicillium Chrysogenum (Penicillium Chrysogenum) is derived from Shandong Lukang Pharmaceutical Co., Ltd., and its microbiological characteristics are as follows: the form of a single colony is: the colony is white, round, The center is raised, and the colony is covered with regular folds; the spores are white or off-white, and the spores are round under the microscope.

[0036] The pretreatment process includes a wall breaking process, a releasing process, an extraction process and a separation process;

[0037] The wall-breaking process refers to adding chitinase to the fermentation broth with penicillin cells for wall-breaking treatment to obtain a treatment liquid with protoplasts of Penicilliu...

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Abstract

The invention discloses a preparation method of 6-aminopenicillanic acid, and belongs to the technical field of biological fermentation, the method comprises the following steps: carrying out pretreatment on penicillin fermentation liquor to obtain treated penicillin fermentation liquor, wherein the pretreatment process comprises a wall breaking process, a release process, an extraction process and a separation process; the method disclosed by the invention has the beneficial effects that a large amount of organic solvents such as butyl acetate and n-butyl alcohol in the later stage of an existing preparation method of 6-aminopenicillanic acid is abandoned, penicillin thalli are creatively subjected to wall breaking treatment by utilizing chitinase, trichloroacetic acid passes through protoplast without cell walls; carrying out denaturation removal on the binding protein bound with penicillin in the protoplast to obtain the penicillin without the binding protein; the problem that the penicillin content in the fermentation liquor is low is solved; an extraction process is creatively introduced, so that the content of penicillin in fermentation liquor is further increased.

Description

Technical field: [0001] The invention belongs to the technical field of biological fermentation, and more specifically relates to a preparation method of 6-aminopenicillanic acid. Background technique: [0002] 6-aminopenicillanic acid (6-APA) is a white flaky crystal, which is an important intermediate for the synthesis of various semi-synthetic penicillins and has a wide range of uses. Using this as a raw material for chemical structure modification and connecting side chains of different structures can produce new semi-synthetic penicillins that are sensitive to penicillin-resistant bacteria and have a wider antibacterial spectrum, such as ampicillin, amoxicillin, and phenoxymethyl penicillin. Penicillin, and other semi-synthetic penicillins with broader antibacterial spectrum. [0003] At present, there are mainly microbial enzyme-catalyzed cleavage methods and chemical cleavage methods to remove penicillin side chains into 6-APA in industry. With the rapid development...

Claims

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Application Information

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IPC IPC(8): C12P37/06C07D499/42C07D499/18
CPCC12P37/06C07D499/42C07D499/18
Inventor 杨冉冉
Owner JINING TECHNICIAN COLLEGE
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