Method for constructing banks of human dental pulp stem cells

A technology of dental pulp stem cells and a construction method, which is applied in the field of biological tissue materials to achieve the effects of enhanced activity, broad application prospects, and enhanced activity

Inactive Publication Date: 2010-06-02
FOURTH MILITARY MEDICAL UNIVERSITY +1
View PDF0 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, various dental materials are mainly used to deal with tooth defect diseases. Although it can prevent the continued development of lesions to a certain extent, it cannot solve the fundamental problem.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1. Preparation of human wisdom tooth pulp stem cells and construction of stem cell bank

[0019] Step 1. Collect basic information: draw donor venous blood and go through testing procedures (ABO / Rh blood type testing, HLA typing testing, syphilis antibody testing, heterohepatitis antigen antibody testing, CMV antibody testing and HIV immune testing, etc.) to determine safety Record and collect the basic information of the donor, including: search code, name, age, ID number, date of birth, race, mailing address and contact information, etc.

[0020] Step 2. Obtain dental pulp stem cells: Obtain two impacted wisdom teeth of the donor and put them into the prepared centrifuge tube containing DMEM culture medium with V / V of 15% calf serum, seal with membrane and transfer to sterile ultra-clean Rinse the tooth tissue repeatedly with pH 7.4 PBS solution to remove surface residual blood and periodontal ligament; transfer to a crusher to crush the tooth, expose the comp...

Embodiment 2

[0023] Example 2. Preparation of dental pulp stem cells from human orthodontically extracted teeth and construction of stem cell bank

[0024] Step 1. Collect basic information: draw donor venous blood and go through testing procedures (ABO / Rh blood type testing, HLA typing testing, syphilis antibody testing, heterohepatitis antigen antibody testing, CMV antibody testing and HIV immune testing, etc.) to determine safety Record and collect the basic information of the donor, including: search code, name, age, ID number, date of birth, race, mailing address and contact information, etc.;

[0025] Step 2. Obtain dental pulp stem cells: Obtain 4 canines of the donor that need to be extracted due to orthodontics, put them into the prepared centrifuge tube containing DMEM culture medium with V / V of 10% calf serum, seal with membrane and transfer Go to a sterile ultra-clean bench, rinse the teeth repeatedly with pH7.4 PBS solution to remove residual blood and periodontal membrane on ...

Embodiment 3

[0028] Example 3. Preparation of human deciduous dental pulp stem cells and construction of stem cell bank

[0029] Step 1. Collect basic information: draw donor venous blood and go through testing procedures (ABO / Rh blood type testing, HLA typing testing, syphilis antibody testing, heterohepatitis antigen antibody testing, CMV antibody testing and HIV immune testing, etc.) to determine safety Record and collect the basic information of the donor, including: search code, name, age, ID number, date of birth, race, mailing address and contact information, etc.

[0030] Step 2. Obtain dental pulp stem cells: Obtain 3 deciduous teeth of the donor that need to be extracted for the replacement of deciduous and permanent teeth, put them into the prepared centrifuge tube containing DMEM culture medium with V / V of 10% calf serum, and seal it with a membrane Transfer to a sterile ultra-clean bench, rinse the tooth tissue repeatedly with pH7.4 PBS solution to remove residual blood and pe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A method for constructing banks of human dental pulp stem cells comprises the steps of acquiring basic information, obtaining dental pulp stem cells, cryopreserving and putting dental pulp stem cells into cell banks and recording and checking information. When the banks of dental pulp stem cells are used, recovery and multiplication culture are carried out in accordance with the actual demands. In the method, the dental pulp stem cells are obtained from dental pulp of waste healthy human teeth and a reserve bank for effective resources is established by using system engineering. A great quantity of dental pulp stem cells with functional activities can be obtained through short-term culture of the human dental pulp stem cells stored in the banks and can be stored for long term without losing the activities. Compared with the prior art, the invention is characterized in that the construction operation specification is simple, easy to grasp, safe and feasible; the bank construction cost is low and the standardization degree is high; and the cells from different age groups adopt different cryopreservation solution so that the activities of the recovered cells are enhanced, thus expanding the sources of the cells and enhancing the activities of the cells. The teeth used in the method are those which are extracted for various reasons and are waste, thus greatly saving the medical resources; therefore, the construction has wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of biological tissue materials, and in particular relates to the preparation of human dental pulp stem cells and the construction method of the cell bank. Background technique [0002] Dental defect and tooth loss due to various reasons have become the main diseases that endanger the health of the oral cavity and even the human body, and the incidence rate is increasing year by year. At present, various dental materials are mainly used to fill tooth defects to deal with tooth defect diseases. Although it can prevent the continued development of lesions to a certain extent, it cannot solve the fundamental problem. Although there are many solutions to tooth loss, the most effective and fundamental way is tooth regeneration. [0003] With the rapid development of tissue regenerative medicine, it brings hope for the effective treatment of dental defects and missing teeth. At present, many studies have used the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074A01N1/02G06F17/30
Inventor 金岩郭维华张勇杰
Owner FOURTH MILITARY MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap