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Isolated culture and neural directional differentiation method of deciduous tooth pulp stem cells

A dental pulp stem cell and directed differentiation technology, which is applied in the field of isolation and culture of dental pulp stem cells in deciduous teeth and directed neural differentiation, can solve the problems of low acquisition rate of highly active cells, complicated separation methods, and long induction period, so as to avoid cell loss, Effect of high cell viability and improved digestion efficiency

Active Publication Date: 2019-12-13
KYBIOSTEM CO LTD
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Problems solved by technology

[0004] In the prior art, to isolate deciduous dental pulp stem cells, an electric drill is generally used to polish the crown to expose the pulp cavity, and the pulp tissue is extracted and then digested with a single collagenase. This separation method is relatively complicated, and the rate of obtaining highly active cells is low; In the process of directed neural differentiation of SHED, the existing technology is usually a one-step method, in which various induction factors are added at the initial stage of culture, and the composition of the medium remains unchanged throughout the induction period, the induction period is longer and the conversion rate is lower

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  • Isolated culture and neural directional differentiation method of deciduous tooth pulp stem cells
  • Isolated culture and neural directional differentiation method of deciduous tooth pulp stem cells
  • Isolated culture and neural directional differentiation method of deciduous tooth pulp stem cells

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Embodiment 1

[0030] Example 1 Isolation and extraction of dental pulp stem cells from deciduous teeth, long-term in vitro culture and directed neural differentiation

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Abstract

The invention provides a long-term in-vitro culture and neural directional differentiation method of deciduous tooth pulp stem cells, which can obtain high-activity primary deciduous tooth pulp stem cells more simply, conveniently and efficiently, and can shorten an induction period and improve the conversion rate in a neural directional differentiation process.

Description

technical field [0001] The invention belongs to the field of stem cell culture, and in particular relates to a method for separating and culturing deciduous dental pulp stem cells and directional nerve differentiation. Background technique [0002] Oral stem cells (Oral stem cells) are a kind of pluripotent cells with self-replication ability, which are considered to be mesenchymal stem cells originating from the neural crest. Dental pulp stem cells of deciduous teeth (SHED) are a kind of oral stem cells, which highly express STRO-1, CD146, Oct4, CD29, CD31, CD44 and other cell markers, but do not express or low express CD14, CD34, CD43, CD45 when appropriate Under certain conditions, it can differentiate into bone cells, chondrocytes, liver cells, nerve-like cells, etc. Compared with bone marrow mesenchymal stem cells (BMMSC), SHED is easier to isolate, proliferates faster, and has stronger cell clone formation ability than BMMSC. [0003] Nerve cells are the basis for th...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12N5/079
CPCC12N5/0618C12N5/0664C12N2501/01C12N2501/11C12N2501/115C12N2501/13C12N2501/60C12N2501/998C12N2506/1361C12N2509/00
Inventor 张晓霜肖锐何倩倩苗天择余倩敏杜宏武
Owner KYBIOSTEM CO LTD
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