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Large scale production technology for dental pulp stem cell

A dental pulp stem cell and production process technology, applied in the field of large-scale production process of dental pulp stem cells, can solve the problems of rapid acquisition, expansion and long-term storage of dental pulp stem cells

Inactive Publication Date: 2014-06-11
上海坤爱生物科技股份有限公司
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  • Abstract
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Problems solved by technology

[0012] Aiming at the problems of rapid acquisition, expansion and long-term storage of dental pulp stem cells used as seed cells, the present invention seeks a more efficient method for culture expansion and long-term storage

Method used

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  • Large scale production technology for dental pulp stem cell

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Embodiment Construction

[0028] The large-scale production process of dental pulp stem cells of the present invention will be described in detail below.

[0029] 1. Isolation and extraction of dental pulp stem cells

[0030] The fresh tooth tissue of the donor was transferred with buffered saline solution containing antibiotics, and the pulp tissue was selected under aseptic conditions and rinsed with buffer solution; the selected pulp tissue was cut into small pieces, and 5-10 times of 0.1%-0.6 % Collagenase was used as the digestive solution, which was blown evenly with a pipette, and placed in a 37°C incubator for 30-180min with shaking. After the digestion is completed, add α-MEM culture solution containing 15% fetal bovine serum to the suspension to stop the digestion, centrifuge at 800~1500r / min for 5~10min, discard the supernatant, add 5~10 times the volume of α-MEM containing 15% The cells were resuspended in the α-MEM medium of fetal bovine serum, and transferred to culture vessels for prima...

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Abstract

The invention provides a large scale production technology for a dental pulp stem cell. The large scale production technology comprises following steps of (1) separating and extracting the dental pulp stem cell; (2) amplifying the dental pulp stem cell through two dimension cultivation; (3) detecting the dental pulp stem cell; (4) cultivating the dental pulp stem cell on a large scale; (5) freeze preserving the dental pulp stem cell; and (6) freeze preserving the dental pulp stem cell for a long term. The invention provides an in vitro large-scale cultivating and amplifying method for a dental pulp stem cell, establishes a long-term, standard and systematized freeze preserving system for a high quality high activity dental pulp stem cell, so that the method can provide a high enough quality stem cell resource for clinic and scientific researches, and has low cost and a wide application prospect.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and specifically relates to a method for culturing and expanding stem cells and cryopreservation, and more specifically relates to a large-scale production process of dental pulp stem cells. Background technique [0002] In 1999, "Science" rated the research results of human embryonic stem cells as the top ten scientific and technological progress in the world that year. In 2000, Time magazine listed it as the top ten scientific and technological achievements in the world at the end of the 20th century. Genome will simultaneously become the most promising field of development and application in the new century. So far, stem cell research has become one of the most striking hot spots in the fields of biology and medicine in recent years. Stem cells are primitive cells that play the role of "backbone" in the growth and development of biological individuals. They are a cell population with sel...

Claims

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Application Information

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IPC IPC(8): C12N5/074C12N5/02A01N1/02
Inventor 傅强陈彦田冯见齐瀚实
Owner 上海坤爱生物科技股份有限公司
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