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Sequential-release double-drug-loading hydrogel as well as preparation method and application thereof

A potion and timing technology, applied in medical science, prosthesis, tissue regeneration, etc., can solve undiscovered problems and achieve low cytotoxicity, good mechanical properties, and suitable for large-scale production

Active Publication Date: 2022-06-24
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the total flavonoids of drynaria have made some progress in the treatment of bone defects and fracture healing, knee osteoarthritis, osteoporosis and distraction osteogenesis, but there is no report of its application in bone repair materials

Method used

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  • Sequential-release double-drug-loading hydrogel as well as preparation method and application thereof
  • Sequential-release double-drug-loading hydrogel as well as preparation method and application thereof
  • Sequential-release double-drug-loading hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A kind of bone repairing hydrogel for promoting the generation of osteoblasts, and its preparation method is as follows:

[0044] 1) Preparation of Rhizoma Drynariae total flavonoid nanospheres

[0045] An appropriate amount of chitosan was dispersed in a 2% (volume) dilute acetic acid aqueous solution, and 1.5% (weight) chitosan solution was prepared after swelling for 24 hours. The total flavonoids of Rhizoma Drynariae were dissolved into the chitosan solution to prepare a chitosan solution containing 5% (weight) total flavonoids of Rhizoma Drynariae. Take 50mL of liquid paraffin and 1.5mL of Span-80 and stir evenly to form an oil phase, slowly add 10mL of the above chitosan solution, continue to stir, emulsify for 2h, and then slowly add 1ml of 25% glutaraldehyde solution of cross-linking agent, stir and cross-link After centrifugation and stratification, the supernatant was removed, and the lower layer was washed 3 times with petroleum ether, isopropanol and ultrap...

Embodiment 2

[0055] A kind of double drug-loading hydrogel, its preparation method is as follows:

[0056] 1) Preparation of total flavonoid microspheres of Rhizoma Drynariae

[0057] Disperse an appropriate amount of sodium alginate in a 2% dilute acetic acid aqueous solution, and swell for 24 hours to prepare a 2% sodium alginate solution. The total flavonoids of Rhizoma Drynariae were dissolved into the sodium alginate solution to prepare a sodium alginate solution containing 8% total flavonoids of Rhizoma Drynariae. Take 50 mL of liquid paraffin and 2 mL of Span-80 and stir evenly to form an oil phase, slowly add 5 mL of the above sodium alginate solution, continue to stir, emulsify for 2 hours, and then slowly add 1 mL of a 25% glutaraldehyde solution of a cross-linking agent, and stir and cross-link for 1 hour; After centrifugation and stratification, the supernatant was removed, and the lower layer was washed three times with petroleum ether, isopropanol and ultrapure water, and fr...

Embodiment 3

[0063] A kind of double drug-loading hydrogel, its preparation method is as follows:

[0064] 1) Preparation of total flavonoid microspheres of Rhizoma Drynariae

[0065] An appropriate amount of chitosan was dispersed in a 2% dilute acetic acid aqueous solution, and 1% chitosan solution was prepared after swelling for 24 hours. The total flavonoids of Rhizoma Drynariae were taken and dissolved in a chitosan solution to prepare a chitosan solution containing 3% total flavonoids of Rhizoma Drynariae. Take 50 mL of soybean oil and 1 mL of Tween 80 and stir evenly to form an oil phase, slowly add 20 mL of the above chitosan solution, continue to stir, emulsify for 2 hours, then slowly add 1 mL of a 25% glutaraldehyde solution as a cross-linking agent, stir and cross-link for 1 hour After centrifugation and stratification, the supernatant was removed, and the lower layer was washed 3 times with petroleum ether, isopropanol and ultrapure water, and freeze-dried to obtain the total...

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Abstract

The invention discloses sequential release double-drug-loading hydrogel, which is prepared by taking PBAE as a carrier material, loading vancomycin and forming the hydrogel with rhizoma drynariae general flavone nano-microspheres, and the material realizes sequential release of vancomycin and rhizoma drynariae general flavone, and has the advantages that the time sequence release of vancomycin and rhizoma drynariae general flavone is realized; and the two can play a synergistic effect to better promote the generation of osteoblasts, thereby having an application prospect in bone repair treatment.

Description

technical field [0001] The invention relates to a bone repair biological material, in particular to a time-sequential release double drug-loaded hydrogel and a preparation method thereof, and also relates to the application of the hydrogel in the preparation of a bone repair material for promoting the generation of osteoblasts. Background technique [0002] Bone defect refers to the absence or loss of bone, such as bone destruction and osteonecrosis. Clinically, the treatment of bone defects requires bone grafting or bone replacement material filling. The construction of substitute materials with physiological functions in vitro is the research focus of bone tissue engineering. The substitute materials can not only repair the defective tissue, but also compensate or replace the related functional activities of tissues and organs. The mechanical properties and biological properties of the tissue are similar, and the material sources should be extensive, cheap and easy to obt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/54A61L27/36A61L27/50A61L27/18
CPCA61L27/52A61L27/54A61L27/3637A61L27/50A61L27/18A61L2430/02A61L2300/406A61L2300/30A61L2300/412A61L2300/45A61L2300/602A61L2300/802C08L67/00
Inventor 许沛虎吕璐徐海星黄志军徐静怡李科比文景程婉婷王思凝林思慧李赟慧王子怡祝宸玥
Owner WUHAN UNIV OF TECH
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