Traditional Chinese medicine composition for enhancing immunity and treating chicken virus infection and preparation method thereof

Abstract

The invention belongs to the field of veterinary traditional Chinese medicines, and provides a traditional Chinese medicine composition for enhancing immunity and treating chicken virus infection and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 18 to 22 parts of radix isatidis, 18 to 22 parts of indigo naturalis, 15 to 20 parts of radix angelicae sinensis, 10 to 15 parts of poria cocos, 10 to 15 parts of rhizoma alismatis, 8 to 12 parts of medicated leaven, 8 to 12 parts of radix salviae miltiorrhizae, 8 to 12 parts of radix codonopsis, 8 to 12 parts of rhizoma cyperi and 4 to 8 parts of liquorice root. The traditional Chinese medicine composition disclosed by the invention has outstanding performance in the aspect of improving IgG and IgM levels, and further shows that the traditional Chinese medicine composition disclosed by the invention can effectively stimulate immune response of an organism and enhance the resistance of the organism to viruses. Animal pharmacological experiments show that the compound has a remarkable treatment effect on chicken infectious disease anemia, avian leukosis and chicken adenovirus infection, provides a new treatment thought for treatment of chicken viral infection, and has a wide application prospect.

Description

Technical Field

[0001] The invention relates to the field of veterinary Chinese medicines, and in particular to a Chinese medicine composition for enhancing immunity and treating chicken virus infection, and a preparation method and application thereof. Background Art

[0002] There are many types of viral infections in chickens, such as chicken infectious anemia virus, avian leukosis / sarcoma virus, adenovirus, infectious bursal disease virus, cell-associated Marek's disease virus infection, etc.

[0003] Chicken infectious anemia is an infectious disease caused by chicken infectious anemia virus, which mainly attacks the bones, thymus and bursa of Fabricius of chicks, and causes aplastic anemia and immunosuppression. The main clinical features of the disease are anemia, mental depression, pale skin, developmental stunting, body punctate hemorrhage, and diarrhea in dying chickens. Hematological examinations show a significant decrease in red blood cells and hemoglobin.

[0004] Avian leukosis is a general term for tumor infectious diseases caused by avian leukosis virus and avian sarcoma virus groups, mainly characterized by the proliferation of certain cell components in hematopoietic tissues. Lymphocytic leukosis is the most common type of disease, and sick chickens are characterized by the development of lymphosarcoma. The most common type of disease is lymphocytic leukosis. Sick chickens are listless, have poor appetite, are emaciated, anemic, have pale and wrinkled combs, are weak all over, and have reduced egg production and egg quality. After the avian leukosis virus infects the flock, it will cause tumor diseases in the flock. More importantly, it will cause the production capacity and immunity of the sick flock to decrease, seriously affecting the egg production quality of laying hens and the growth of broilers, and seriously threatening the healthy, sustainable and efficient development of the poultry industry.

[0005] Adenovirus disease in chickens is caused by infection with chicken adenovirus, and the main manifestations are impaired immune system and organ damage. The severity of chicken adenovirus infection and the individual conditions of the chickens play an important role in the choice of treatment. Antibiotic therapy has a strong antibacterial effect and a rapid therapeutic effect. It is suitable for more serious conditions or when the infection needs to be quickly controlled. However, antibiotics are prone to drug resistance and may have side effects on chickens. In contrast, Chinese medicine treatment is relatively superior in efficacy.

[0006] When poultry suffers from immunosuppressive diseases due to infectious or non-infectious factors, their immune organs will be damaged, especially the central immune organs such as the bursa of Fabricius and the thymus. As the immune function of poultry declines and is lost, its disease resistance also decreases significantly. Through the protection and weight gain of the immune organs of the chicken body by Chinese medicine and its effective ingredients, the immune cells are activated, the non-specific immune enhancement effect is achieved, the immune system is activated, and the immunity of the chicken body is improved.

[0007] Developing a Chinese medicine composition that can effectively resist viruses and prevent and treat epidemic diseases is of great significance for preventing and treating chicken virus infection. Summary of the invention

[0008] The main purpose of the present invention is to provide a Chinese medicine composition for preventing and treating chicken virus infection and enhancing immunity, a preparation method and application thereof, so as to solve the problem of lack of effective Chinese medicine composition for resisting viruses and preventing and treating diseases in the prior art.

[0009] In order to achieve the above-mentioned object, according to one aspect of the present invention, a Chinese medicine composition for preventing and treating chicken virus infection is provided for enhancing immunity. The Chinese medicine composition for preventing and treating chicken virus infection is composed of the following components by weight: 18-22 parts of Isatis root, 18-22 parts of Indigo naturalis, 15-20 parts of Angelica sinensis, 10-15 parts of Poria cocos, 10-15 parts of Alisma orientalis, 8-12 parts of Shenqu, 8-12 parts of Salvia miltiorrhiza, 8-12 parts of Codonopsis pilosula, 8-12 parts of Cyperus rotundus and 4-8 parts of Licorice.

[0010] In a preferred embodiment of the present invention, the Chinese medicine composition is composed of the following components by weight: 22 parts of Isatis root, 18 parts of Indigo naturalis, 20 parts of Angelica sinensis, 10 parts of Poria cocos, 15 parts of Alisma orientalis, 8 parts of Shenqu, 12 parts of Salvia miltiorrhiza, 12 parts of Codonopsis pilosula, 8 parts of Cyperus rotundus and 8 parts of Licorice.

[0011] In a preferred embodiment of the present invention, the Chinese medicine composition is composed of the following components by weight: 18 parts of Isatis root, 22 parts of Indigo naturalis, 15 parts of Angelica sinensis, 15 parts of Poria cocos, 10 parts of Alisma orientalis, 12 parts of Shenqu, 8 parts of Salvia miltiorrhiza, 8 parts of Codonopsis pilosula, 12 parts of Cyperus rotundus and 4 parts of Licorice.

[0012] In a preferred embodiment of the present invention, the Chinese medicine composition is composed of the following components by weight: 20 parts of Isatis root, 20 parts of Indigo naturalis, 18 parts of Angelica sinensis, 12 parts of Poria cocos, 12 parts of Alisma orientalis, 10 parts of Shenqu, 10 parts of Salvia miltiorrhiza, 10 parts of Codonopsis pilosula, 10 parts of Cyperus rotundus and 6 parts of Licorice.

[0013] According to another aspect of the present invention, a method for preparing the traditional Chinese medicine composition for preventing and treating chicken virus infection is provided, comprising the following steps: weighing the above traditional Chinese medicines, mixing and decocting in water for 1-3 times, each time for 30-60 minutes, filtering and combining the filtrate, concentrating the obtained filtrate and drying.

[0014] Furthermore, the preparation method of the Chinese medicine composition for preventing and treating chicken virus infection comprises the following steps: weighing the above Chinese medicines, mixing them, decocting them in water for 2 times, each time for 50 minutes, filtering and combining the filtrates, concentrating the obtained filtrates and drying them.

[0015] According to another aspect of the present invention, provided is the use of the traditional Chinese medicine composition for preventing and treating chicken virus infection in the preparation of a drug for preventing and treating chicken virus infection.

[0016] The invention also provides the use of the Chinese medicinal composition for preventing and treating chicken virus infection in preparing medicine for preventing and treating chicken infectious anemia, avian leukosis or chicken adenovirus infection.

[0017] The Chinese medicine composition of the present invention is composed of Radix Isatidis, Indigo Naturalis, Angelica Sinensis, Poria, Rhizoma Alismatis, Shenqu, Salvia Miltiorrhiza, Codonopsis Pilosula, Rhizoma Cyperi and Licorice. The properties, flavors and meridians of each Chinese medicine component are as follows: Isatis root: bitter in taste, cold in nature, enters the heart and stomach meridians, clears away heat and detoxifies, cools blood and relieves sore throat; used for wind-heat colds, sore throats, mouth and tongue ulcers, and yellow and swollen sores.

[0018] Indigo: salty in taste, cold in nature, enters the liver meridian, clears away heat and detoxifies, cools blood and eliminates freckles, purges fire and calms the nerves; used for fever due to febrile diseases, rashes, vomiting blood, hemoptysis, sore throat and mouth ulcers, epilepsy in children, sores, erysipelas, snake and insect bites, etc.

[0019] Angelica: spicy and bitter in taste, warm in nature, enters the heart, liver, and spleen meridians, replenishes blood and promotes blood circulation, regulates menstruation and relieves pain, moisturizes and lubricates the intestines; used for irregular menstruation, amenorrhea, dysmenorrhea, accumulation of masses, metrorrhagia, abdominal pain due to deficiency and cold, paralysis, numbness of the skin, dry intestines and difficulty in defecation, heaviness after dysentery, carbuncles, ulcers, and injuries from falls.

[0020] Poria: sweet and light in taste, neutral in nature, enters the heart, lung, spleen, and kidney meridians, promotes diuresis and eliminates dampness, benefits the spleen and stomach, calms the mind and soothes the nerves, and enhances the body's immune function. Poria polysaccharide has obvious anti-tumor and liver protection effects.

[0021] Alisma: sweet and light in taste, cold in nature, enters the kidney and bladder meridians, promotes diuresis and eliminates dampness, relieves heat, resolves turbidity and reduces fat; used for urinary problems, edema, diarrhea, oliguria, phlegm and fluid, dizziness, and painful stranguria caused by heat.

[0022] Shenqu: bitter in taste, warm in nature, enters the spleen, stomach, and large intestine meridians, strengthens the spleen and aids digestion, regulates qi and eliminates dampness, and relieves exterior symptoms; used for colds, food stagnation, stomach bloating, indigestion, diarrhea, and is good at eliminating starch, melons and fruits.

[0023] Salvia miltiorrhiza: bitter in taste, slightly cold in nature, enters the heart and liver meridians, promotes blood circulation and removes blood stasis, cools blood and eliminates carbuncles, clears the heart and relieves restlessness; it is used for symptoms such as irregular menstruation, blood stasis and amenorrhea, abdominal pain due to postpartum blood stasis, heart and abdominal pain, accumulation of lumps and masses, and limb pain.

[0024] Codonopsis: sweet in taste, neutral in nature, enters the lung and spleen meridians, tonifies the middle and replenishes qi, quenches thirst, strengthens the spleen and benefits the lungs, nourishes blood and produces body fluid; used for spleen and lung qi deficiency, poor appetite and fatigue, cough and weak wheezing, insufficient qi and blood, sallow complexion, palpitations and shortness of breath, thirst due to loss of body fluid, and internal heat and thirst.

[0025] Cyperus rotundus: It tastes pungent, slightly bitter and sweet, and is neutral in nature. It enters the lung, liver, spleen, and stomach meridians. It can soothe the liver and relieve depression, regulate qi and relieve fullness, regulate menstruation and relieve pain. It is used for liver depression and qi stagnation, chest and flank distension and pain, hernia pain, breast distension and pain, spleen and stomach qi stagnation, abdominal distension and pain, irregular menstruation, and amenorrhea and dysmenorrhea.

[0026] Licorice: sweet in taste, neutral in nature, enters the heart, lung, spleen, and stomach meridians, tonifies the spleen and replenishes Qi, clears away heat and detoxifies, removes phlegm and relieves cough, relieves pain, and harmonizes various medicines; it is used for spleen and stomach weakness, fatigue, palpitations and shortness of breath, cough with excessive sputum, cramps and pain in the abdomen and limbs, carbuncles and sores, and relieves the toxicity and potency of drugs.

[0027] The Chinese medicine composition of the present invention targets the pathogenesis and external manifestations of viral infection, follows the TCM treatment principle of "treating both the symptoms and the root cause", and achieves the effects of clearing viruses and promoting body growth by resisting viruses, adjusting immune function, strengthening the body, protecting the liver and promoting bile secretion.

[0028] Compared with the prior art, the present invention has the following advantages: The Chinese medicine composition of the present invention has outstanding performance in improving IgG and IgM levels, indicating that the Chinese medicine composition of the present invention can effectively stimulate the body's immune response and enhance the body's resistance to viruses. The Chinese medicine composition of the present invention has excellent effects in inhibiting virus reproduction, eliminating pathogens and improving immune function.

[0029] The Chinese medicinal composition of the invention has a significant therapeutic effect on chicken infectious anemia, avian leukosis and chicken adenovirus infection, and provides a new therapeutic idea for the treatment of chicken viral infection.

[0030] The Chinese medicinal composition of the invention has a high cure rate for treating chicken viral infection and has broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 Comparison of IgG levels in each group after treatment, compared with group A, * P<0.05, ** P<0.01; compared with group B, # P<0.05.

[0032] Figure 2 Comparison of IgM levels in each group after treatment, compared with group A, * P<0.05, ** P<0.01; compared with group B, # P<0.05. DETAILED DESCRIPTION

[0033] In order to verify the efficacy of the Chinese medicine composition of the present invention, the inventors conducted a pharmacodynamics test study. It should be noted that the drugs selected for the pharmacodynamics test study of the present invention are drugs obtained by the representative formula of the present invention and its preparation method. Due to space limitations, the tests and results involved in the drugs obtained by other formulas and preparation methods included in the present invention are not listed here one by one.

[0034] Example 1: A Chinese medicine composition for preventing and treating chicken virus infection, the formula and preparation method are as follows: 20 parts of Radix Isatidis, 20 parts of Indigo Naturalis, 18 parts of Angelica Sinensis, 12 parts of Poria, 12 parts of Rhizoma Alismatis, 10 parts of Radix Angelicae Sinensis, 10 parts of Radix Salviae Miltiorrhizae, 10 parts of Codonopsis Pilosulae, 10 parts of Rhizoma Cyperi and 6 parts of Radix Glycyrrhiza

[0035] The preparation method is as follows: weigh the above Chinese medicines, mix them, decoct them in water for 2 times, each time for 50 minutes, filter them, combine the filtrates, concentrate the filtrates, and then dry them to obtain the product.

[0036] Example 2: A Chinese medicine composition for preventing and treating chicken virus infection, the formula and preparation method are as follows: 22 parts of Radix Isatidis, 18 parts of Indigo Naturalis, 20 parts of Angelica Sinensis, 10 parts of Poria, 15 parts of Rhizoma Alismatis, 8 parts of Radix Angelicae Pubescentis, 12 parts of Salvia Miltiorrhizae, 12 parts of Codonopsis Pilosulae, 8 parts of Rhizoma Cyperi and 8 parts of Radix Glycyrrhizae.

[0037] The preparation method is as follows: weigh the above Chinese medicines, mix them, decoct them in water for 3 times, each time for 30 minutes, filter them, combine the filtrates, concentrate the filtrates, and then dry them to obtain the product.

[0038] Example 3: A Chinese medicine composition for preventing and treating chicken virus infection, the formula and preparation method are as follows: 18 parts of Radix Isatidis, 22 parts of Indigo Naturalis, 15 parts of Angelica Sinensis, 15 parts of Poria, 10 parts of Rhizoma Alismatis, 12 parts of Radix Angelicae Sinensis, 8 parts of Salvia Miltiorrhizae, 8 parts of Codonopsis Pilosulae, 12 parts of Rhizoma Cyperi and 4 parts of Radix Glycyrrhizae.

[0039] The preparation method is as follows: weigh the above Chinese medicines, mix them, decoct them in water for one time, each time for 60 minutes, filter them and combine the filtrates, concentrate the filtrates and dry them to obtain the product.

[0040] Example 4: A Chinese medicine composition for preventing and treating chicken virus infection, the formula and preparation method are as follows: 18 parts of Radix Isatidis, 20 parts of Indigo Naturalis, 20 parts of Angelica Sinensis, 10 parts of Poria, 12 parts of Rhizoma Alismatis, 10 parts of Radix Angelicae Pubescentis, 8 parts of Salvia Miltiorrhizae, 12 parts of Codonopsis Pilosulae, 10 parts of Rhizoma Cyperi and 6 parts of Radix Glycyrrhizae.

[0041] The preparation method is as follows: weigh the above Chinese medicines, mix them, decoct them in water for 2 times, each time for 40 minutes, filter them, combine the filtrates, concentrate the filtrates, and then dry them to obtain the product.

[0042] Example 5: A Chinese medicine composition for preventing and treating chicken virus infection, the formula and preparation method are as follows: 22 parts of Radix Isatidis, 18 parts of Indigo Naturalis, 20 parts of Angelica Sinensis, 10 parts of Poria, 15 parts of Rhizoma Alismatis, 12 parts of Radix Angelicae Sinensis, 8 parts of Salvia Miltiorrhizae, 10 parts of Codonopsis Pilosulae, 10 parts of Rhizoma Cyperi and 8 parts of Radix Glycyrrhizae.

[0043] The preparation method is as follows: weigh the above Chinese medicines, mix them, decoct them in water for 3 times, each time for 30 minutes, filter them, combine the filtrates, concentrate the filtrates, and then dry them to obtain the product.

[0044] Comparative Example 1: A Chinese medicine composition for preventing and treating chicken virus infection, the formula and preparation method are as follows: 18 parts of Chinese Angelicae Sinensis, 12 parts of Poria, 12 parts of Alisma Orientalis, 10 parts of Radix Angelicae Sinensis, 10 parts of Salvia miltiorrhiza, 10 parts of Codonopsis pilosula, 10 parts of Cyperus rotundus and 6 parts of Licorice.

[0045] The preparation method is the same as that of Example 1.

[0046] Comparative Example 2: A Chinese medicine composition for preventing and treating chicken virus infection, the formula and preparation method are as follows: 20 parts of Radix Isatidis, 20 parts of Indigo Naturalis, 12 parts of Poria, 12 parts of Rhizoma Alismatis, 10 parts of Salvia Miltiorrhiza, 10 parts of Codonopsis Pilosula, 10 parts of Rhizoma Cyperi and 6 parts of Radix Glycyrrhizae.

[0047] The preparation method is the same as that of Example 1.

[0048] Comparative Example 3: A Chinese medicine composition for preventing and treating chicken virus infection, the formula and preparation method are as follows: 20 parts of Radix Isatidis, 20 parts of Indigo Naturalis, 18 parts of Radix Astragali, 12 parts of Poria, 12 parts of Rhizoma Alismatis, 10 parts of Malt, 10 parts of Salvia Miltiorrhiza, 10 parts of Ginseng, 10 parts of Rhizoma Cyperi and 6 parts of Licorice.

[0049] The preparation method is the same as that of Example 1.

[0050] Efficacy Example 1 Study on the effect of the Chinese medicine composition of the present invention on chicken infectious anemia Sick chickens diagnosed with chicken infectious anemia according to clinical symptoms, autopsy changes and laboratory tests. Chickens with symptoms and chickens without symptoms were isolated. 300 sick chickens aged 20 days and weighing 1.1-1.3 kg were selected and randomly divided into 6 groups, 50 in each group, respectively: model control group (referred to as group A), embodiment 1 group (referred to as group B), embodiment 2 group (referred to as group C), comparative example 1 group (referred to as group D), comparative example 2 group (referred to as group E), comparative example 3 group (referred to as group F).

[0051] Group B and Group C were fed with the Chinese medicine compositions of Example 1 and Example 2, respectively, and Group D, Group E and Group F were fed with the Chinese medicine compositions of Comparative Example 1, Comparative Example 2 and Comparative Example 3, respectively, with the dosage of each group being 0.5 g / day per chicken for 14 days of treatment. Group A was not fed with any medicine.

[0052] The morbidity, cure rate and mortality rate of chickens in each group were observed and recorded.

[0053] Table 1 Incidence, cure rate and mortality rate of each group The Chinese medicine composition of the present invention has a significant therapeutic effect on chicken infectious anemia, reduces mortality and improves cure rate. The therapeutic effects of Example 1 and Example 2 are significantly better than those of Comparative Example 1 and Comparative Example 2.

[0054] Efficacy Example 2 Study on the effect of the Chinese medicine composition of the present invention on chicken adenovirus infection 1. Materials and Methods According to clinical symptoms, autopsy changes and laboratory tests, sick chickens were diagnosed as infected with chicken adenovirus. Chickens with symptoms and chickens without symptoms were isolated and treated. 180 sick chickens were selected and randomly divided into 6 groups, 30 in each group, respectively: model control group (denoted as group A), embodiment 1 group (denoted as group B), embodiment 2 group (denoted as group C), comparative example 1 group (denoted as group D), comparative example 2 group (denoted as group E), comparative example 3 group (denoted as group F).

[0055] Group B and Group C were fed with the Chinese medicine compositions of Example 1 and Example 2, respectively, and Group D, Group E and Group F were fed with the Chinese medicine compositions of Comparative Example 1, Comparative Example 2 and Comparative Example 3, respectively, with the dosage of each group being 0.5 g / kg per chicken per day for 10 days. Group A was not fed with any medicine.

[0056] 2. Laboratory testing Blood samples were collected from the chickens before, during and after treatment for immune function testing, which mainly included antibody level testing and immune cell count testing.

[0057] Observe and record the cure rate and mortality rate of chickens in each group.

[0058] 3. Data Analysis use The software processes the data and measures the data. The comparison among multiple groups was performed by one-way analysis of variance, and the comparison among groups was performed by Pairwise comparisons were performed, and P < 0.05 was considered statistically significant.

[0059] 4. Experimental Results 4.1 Immune function testing The results are as follows Figure 1 , Figure 2 As shown, the Chinese medicine composition of the present invention can effectively stimulate the body's immune response and significantly improve the levels of IgG and IgM. The effect of enhancing the body's resistance to viruses in Example 1 and Example 2 is significantly better than that in Comparative Example 1 and Comparative Example 2.

[0060] 4.2 Cure rate and mortality rate of chickens in each group Table 2 Cure rate and mortality rate of each group The results are shown in Table 2. The Chinese medicine composition of the present invention has a significant therapeutic effect on chicken adenovirus infection, reduces mortality and improves cure rate. The therapeutic effects of Example 1 and Example 2 are significantly better than those of Comparative Example 1 and Comparative Example 2.

[0061] The Chinese medicine composition of the present invention has outstanding performance in improving IgG and IgM levels, indicating that the Chinese medicine composition of the present invention can effectively stimulate the body's immune response and enhance the body's resistance to viruses. The Chinese medicine composition of the present invention has excellent performance in inhibiting virus reproduction, eliminating pathogens and improving immune function.

[0062] Efficacy Example 3 Study on the effect of the Chinese medicine composition of the present invention on avian leukosis 240 sick chickens that were positive for avian leukosis virus by ELISA meconium detection were selected and randomly divided into 4 groups, with 60 chickens in each group, namely: model control group (denoted as group A), embodiment 1 group (denoted as group B), embodiment 2 group (denoted as group C), and embodiment 3 group (denoted as group D).

[0063] Group B, Group C, and Group D were fed with the Chinese medicine composition of Example 1, Example 2, and Example 3, respectively, with the dosage of each group being 0.5 g / day per chicken, and the treatment lasted for 14 days. Group A was not fed with any medicine.

[0064] Observe and record the cure rate and mortality rate of chickens in each group.

[0065] Table 3 Cure rate and mortality rate of each group The results are shown in Table 3. The Chinese medicine composition of the present invention has a significant therapeutic effect on avian leukosis, reduces the mortality rate and improves the cure rate.

[0066] The above embodiments are not any form of limitation to the present invention. For those skilled in the art, any modification, replacement, etc. made within the scope of the technical solution of the present invention based on the technical essence of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A Chinese medicinal composition for treating chicken virus infection and enhancing immunity, characterized in that: The Chinese medicine composition consists of the following components by weight: 18-22 parts of isatis root, 18-22 parts of indigo, 15-20 parts of angelica, 10-15 parts of tuckahoe, 10-15 parts of oriental rhizome, 8-12 parts of Shenqu, 8-12 parts of salvia miltiorrhiza, 8-12 parts of codonopsis pilosula, 8-12 parts of cyperus rotundus and 4-8 parts of liquorice.

2. The Chinese medicinal composition for treating chicken virus infection for enhancing immunity according to claim 1, characterized in that: The Chinese medicine composition consists of the following components by weight: 22 parts of Radix Isatidis, 18 parts of Indigo Naturalis, 20 parts of Angelica Sinensis, 10 parts of Poria, 15 parts of Rhizoma Alismatis, 8 parts of Radix Angelicae Pubescentis, 12 parts of Salviae Miltiorrhizae, 12 parts of Codonopsis Pilosulae, 8 parts of Cyperus Rhizoma and 8 parts of Licorice.

3. The Chinese medicinal composition for treating chicken virus infection for enhancing immunity according to claim 1, characterized in that: The Chinese medicine composition consists of the following components by weight: 18 parts of Radix Isatidis, 22 parts of Indigo Naturalis, 15 parts of Angelica Sinensis, 15 parts of Poria, 10 parts of Rhizoma Alismatis, 12 parts of Radix Angelicae Pubescentis, 8 parts of Salviae Miltiorrhizae, 8 parts of Codonopsis Pilosulae, 12 parts of Rhizoma Cyperi and 4 parts of Licorice.

4. The Chinese medicinal composition for treating chicken virus infection for enhancing immunity according to claim 1, characterized in that: The Chinese medicine composition consists of the following components by weight: 20 parts of Radix Isatidis, 20 parts of Indigo Naturalis, 18 parts of Angelica Sinensis, 12 parts of Poria, 12 parts of Rhizoma Alismatis, 10 parts of Radix Angelicae Pubescentis, 10 parts of Salvia Miltiorrhizae, 10 parts of Codonopsis Pilosulae, 10 parts of Rhizoma Cyperi and 6 parts of Licorice.

5. A method for preparing a Chinese medicinal composition for treating chicken virus infection for enhancing immunity as claimed in any one of claims 1 to 4, characterized in that: The preparation method comprises the following steps: weighing the components according to any one of claims 1 to 4, mixing and decocting for 1 to 3 times, each time for 30 to 60 minutes, filtering and combining the filtrates, concentrating the obtained filtrates and drying them.

6. The method for preparing the immunity-enhancing Chinese medicinal composition for treating chicken virus infection according to claim 5, characterized in that: The preparation method comprises the following steps: weighing the above Chinese medicines, mixing them and decocting them in water for extraction twice, each time for 50 minutes, filtering and combining the filtrates, concentrating the obtained filtrates and drying them to obtain the product.

7. Use of the immunity-enhancing Chinese medicinal composition for treating chicken virus infection as claimed in any one of claims 1 to 4 in the preparation of drugs for preventing and treating chicken virus infection.

8. Use of the immunity-enhancing Chinese medicinal composition for treating chicken virus infection as claimed in any one of claims 1 to 4 in the preparation of drugs for preventing and treating chicken infectious anemia, avian leukosis or chicken adenovirus infection.

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