Antibiotic-free feed for marking prawns in artificial seawater and circulating water and preparation method of antibiotic-free feed

Abstract

The invention provides an antibiotic-free feed for artificial seawater circulating water prawn roughing and a preparation method thereof, and relates to the technical field of seawater prawn feed preparation. The antibiotic-free feed is prepared by compounding spirulina powder, high gluten flour, krill powder, tenebrio molitor powder, clostridium ethanol protein powder, fish oil, a yeast extract, monopotassium phosphate, liquid phospholipid, mulberry leaf powder, mung bean powder, haematococcus pluvialis powder, selenium yeast and fish soluble. The compound feed overcomes the defects in the prior art, solves the problems that an existing compound feed is low in survival rate, poor in environmental change resistance and frequent in use of animal protection products in the shrimp seed thickening process, and comprehensively improves the culture economic benefits of the seawater prawns.

Description

Technical Field

[0001] The invention relates to the technical field of seawater shrimp feed preparation, and in particular to a standard antibiotic-free feed for shrimps in artificial seawater circulating water and a preparation method thereof. Background Art

[0002] In the process of shrimp roughing using artificial seawater circulating water, the shrimp fry are under great stress when transported from the hatchery to the breeding roughing pool, and cannot adapt well to the environment. They are fed with conventional compound feeds, have low survival rates, and poor resistance to environmental changes. This causes economic losses and water treatment burdens in production. Spirulina powder is a natural algae raw material that can be well ingested by shrimp fry, but due to its poor nutritional composition, the shrimp fry grows slowly during the growth process. On this basis, it is crucial to develop and configure a feed for shrimp roughing using artificial seawater circulating water. Summary of the invention

[0003] In view of the shortcomings of the existing technology, the present invention provides an antibiotic-free feed suitable for shrimp fry standardization using artificial seawater factory circulating water, which solves the problems of low survival rate of existing compound feeds in the shrimp fry standardization process, poor resistance to environmental changes, and frequent use of animal health products.

[0004] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0005] The invention discloses a crude antibiotic-free feed for shrimps in artificial seawater circulating water. The raw materials of the antibiotic-free feed consist of the following materials in parts by weight: 50-65 parts of spirulina powder, 12-22 parts of high-gluten flour, 5-12 parts of krill powder, 3-10 parts of yellow mealworm powder, 2-8 parts of ethanol clostridium protein powder, 1-3 parts of fish oil, 1-3 parts of yeast extract, 1-2 parts of potassium dihydrogen phosphate, 1-3 parts of liquid lecithin, 0.2-1 parts of mulberry leaf powder, 0.1-1 parts of mung bean powder, 0.1-1 parts of Haematococcus pluvialis powder, 0.05-0.2 parts of yeast selenium and 0.05-0.5 parts of fish soluble slurry.

[0006] Preferably, the raw materials of the antibiotic-free feed are composed of the following substances in parts by weight: 59 parts of spirulina powder, 15 parts of high-gluten flour, 7 parts of krill meal, 6 parts of mealworm powder, 5 parts of glycolic acid bacteria protein powder, 2 parts of fish oil, 2 parts of yeast extract, 1.5 parts of potassium dihydrogen phosphate, 1.4 parts of liquid lecithin, 0.5 parts of mulberry leaf powder, 0.2 parts of mung bean powder, 0.2 parts of Haematococcus pluvialis powder, 0.1 parts of yeast selenium, and 0.1 parts of fish slurry.

[0007] Preferably, the parameter indicators of the spirulina powder are: protein content is 61%-62%, fat content is less than 4%, moisture content is less than 10%, ash content is less than 10%, and iron content is less than 800 mg / kg.

[0008] Preferably, the protein content of the ethanolic Clostridium protein powder is above 80%.

[0009] The method for preparing the standard antibiotic-free feed for shrimps using artificial seawater circulating water comprises the following steps:

[0010] (1) crushing krill powder and yellow mealworm powder to obtain crushed krill powder and crushed yellow mealworm powder for later use;

[0011] (2) mixing the crushed krill powder and the crushed mealworm powder with spirulina powder, high-gluten flour, ethanolic Clostridium protein powder, fish oil, yeast extract, potassium dihydrogen phosphate, liquid lecithin, mulberry leaf powder, mung bean powder, Haematococcus pluvialis powder, yeast selenium, and fish slurry, and mixing them evenly, and then crushing them in an ultrafine grinder to obtain a composite crushed material for use;

[0012] (3) The composite crushed material is conditioned in a conditioning device to control the moisture content to 25%-30%, and then granulated in a single screw extruder to obtain granular material for later use;

[0013] (4) Drying the above pellets to obtain antibiotic-free feed.

[0014] Preferably, in step (1), the crushed krill powder and the crushed mealworm powder are sieved through a 40-mesh sieve.

[0015] Preferably, in step (2), the composite crushed material is sieved through an 80-mesh sieve.

[0016] Preferably, the temperature entering the single screw extruder in step (3) is 100-120°C.

[0017] Preferably, the drying temperature in step (4) is 90-100°C.

[0018] The present invention provides an artificial seawater circulating water standard antibiotic-free feed for shrimp and a preparation method thereof, which has the advantages of:

[0019] (1) The feed of the present invention is prepared according to the growth characteristics and nutritional requirements of shrimp, and can improve the survival rate of shrimp fry during the roughing process. The spirulina powder added to the feed can make the shrimp fry adapt to the circulating water aquaculture environment to the greatest extent. The added spirulina powder and Haematococcus pluvialis powder can improve the intestinal immune barrier and enhance the immune function. The added mulberry leaf powder and mung bean powder can have an antiviral effect.

[0020] (2) The antibiotic-free feed for circulating water shrimp of the present invention has a reasonable nutritional ratio, can promote growth, enhance immunity, resist stress, and protect the intestinal tract of shrimp. The feed has good stability in water and causes little pollution to the artificial seawater circulating water body. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention is clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] Embodiment 1:

[0023] Preparation of antibiotic-free feed for shrimps in artificial seawater circulating water:

[0024] (1) Prepare the following materials in parts by weight: 59 parts of spirulina powder (protein 61-62%, fat less than 4%, moisture less than 10%, ash less than 10%, iron less than 800 mg / kg), 15 parts of high-gluten flour, 7 parts of krill powder, 6 parts of mealworm powder, 5 parts of glycolic acid bacteria protein powder (protein content greater than 80%), 2 parts of fish oil, 2 parts of yeast extract, 1.5 parts of potassium dihydrogen phosphate, 1.4 parts of liquid lecithin, 0.5 parts of mulberry leaf powder, 0.2 parts of mung bean powder, 0.2 parts of Haematococcus pluvialis powder, 0.1 parts of yeast selenium, and 0.1 parts of fish slurry;

[0025] (2) grinding krill powder and mealworm powder and passing them through a 40-mesh sieve;

[0026] (3) mixing the sieved krill powder and mealworm powder with other components in the raw materials;

[0027] (4) The mixed raw materials are crushed again in an ultrafine grinder to pass through an 80-mesh sieve;

[0028] (5) The ultrafinely crushed raw material is conditioned in a modulator, and the moisture content of the conditioned raw material is 25%-30%. The raw material is then pelletized in a single screw extruder at a temperature of 100°C;

[0029] (6) Dry the obtained pellets at 90°C to obtain antibiotic-free feed.

[0030] Embodiment 2:

[0031] Preparation of antibiotic-free feed for shrimps in artificial seawater circulating water:

[0032] (1) Prepare the following materials in parts by weight: 65 parts of spirulina powder (protein 61-62%, fat less than 4%, moisture less than 10%, ash less than 10%, iron less than 800 mg / kg), 22 parts of high-gluten flour, 12 parts of krill powder, 10 parts of mealworm powder, 8 parts of glycolic acid bacteria protein powder (protein content greater than 80%), 3 parts of fish oil, 3 parts of yeast extract, 2 parts of potassium dihydrogen phosphate, 3 parts of liquid lecithin, 1 part of mulberry leaf powder, 1 part of mung bean powder, 1 part of Haematococcus pluvialis powder, 0.2 parts of yeast selenium, and 0.5 parts of fish slurry;

[0033] (2) grinding krill powder and mealworm powder and passing them through a 40-mesh sieve;

[0034] (3) mixing the sieved krill powder and mealworm powder with other components in the raw materials;

[0035] (4) The mixed raw materials are crushed again in an ultrafine grinder to pass through an 80-mesh sieve;

[0036] (5) The ultrafinely crushed raw material is conditioned in a modulator, and the moisture content of the conditioned raw material is 25%-30%. The raw material is then pelletized in a single screw extruder at a temperature of 120°C;

[0037] (6) Dry the obtained pellets at 100°C to obtain antibiotic-free feed.

[0038] Embodiment 3:

[0039] Preparation of antibiotic-free feed for shrimps in artificial seawater circulating water:

[0040] (1) Prepare the following materials in parts by weight: 50 parts of spirulina powder (protein 61-62%, fat less than 4%, moisture less than 10%, ash less than 10%, iron less than 800 mg / kg), 12 parts of high-gluten flour, 5 parts of krill powder, 3 parts of mealworm powder, 2 parts of glycolic acid bacteria protein powder (protein content greater than 80%), 1 part of fish oil, 1 part of yeast extract, 1 part of potassium dihydrogen phosphate, 1 part of liquid lecithin, 0.2 parts of mulberry leaf powder, 0.1 parts of mung bean powder, 0.1 parts of Haematococcus pluvialis powder, 0.05 parts of yeast selenium, and 0.05 parts of fish slurry;

[0041] (2) grinding krill powder and mealworm powder and passing them through a 40-mesh sieve;

[0042] (3) mixing the sieved krill powder and mealworm powder with other components in the raw materials;

[0043] (4) The mixed raw materials are crushed again in an ultrafine grinder to pass through an 80-mesh sieve;

[0044] (5) The ultrafinely crushed raw material is conditioned in a modulator, and the moisture content of the conditioned raw material is 25%-30%. The raw material is then pelletized in a single screw extruder at a temperature of 110° C.;

[0045] (6) Dry the obtained pellets at 95°C to obtain antibiotic-free feed.

[0046] experiment:

[0047] 1200 P5 shrimp fry of whiteleg shrimp were selected and placed in 4 net frames with circulating water, the frames contained artificially prepared seawater, and there was no biological bait in the water. 300 shrimp fry in each net frame were fed with commercial feed (crude protein 48%, crude fat ≥ 6%), feed prepared by Example 1, Example 2, and Example 3 respectively. They were fed 4 times a day at 8:00, 12:00, 18:00, and 21:00 respectively. No water change or sewage discharge was performed every day, and the aeration and oxygenation were increased continuously. The natural light was indoors, the dissolved oxygen content was greater than 6.0 mg / L, the ammonia nitrogen was less than 0.2 mg / L, the water temperature was 28-30°C, the salinity was 15-20‰, and the pH was 7.8-8.0. After feeding for 4 weeks, the samples were weighed to obtain the average weight of whiteleg shrimp in each pool. After the shrimps in each pool were weighed, the survival rate of each pool was calculated by weighing the samples. The results are shown in Table 1 below.

[0048] Table 1

[0049] Group Average weight after 4 weeks of feeding (g) Survival rate calculated based on average weight Commodity materials 0.48 30.1％ Example 1 Antibiotic-free feed 0.30 54.4％ Example 2 Antibiotic-free feed 0.32 62.3％ Example 3 Antibiotic-free feed 0.41 71.5％

[0050] It can be seen from the above table that the feeds prepared in Examples 1-3 can significantly improve the survival rate of shrimps compared to ordinary commercial feeds.

[0051] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An antibiotic-free feed for shrimps in artificial seawater circulation water, characterized in that: The raw materials of the antibiotic-free feed are composed of the following materials in parts by weight: 50-65 parts of spirulina powder, 12-22 parts of high-gluten flour, 5-12 parts of krill powder, 3-10 parts of yellow mealworm powder, 2-8 parts of ethanol clostridium protein powder, 1-3 parts of fish oil, 1-3 parts of yeast extract, 1-2 parts of potassium dihydrogen phosphate, 1-3 parts of liquid lecithin, 0.2-1 parts of mulberry leaf powder, 0.1-1 parts of mung bean powder, 0.1-1 parts of Haematococcus pluvialis powder, 0.05-0.2 parts of yeast selenium, and 0.05-0.5 parts of fish soluble slurry.

2. The antibiotic-free feed for shrimps according to claim 1, characterized in that: The raw materials of the antibiotic-free feed are composed of the following substances in parts by weight: 59 parts of spirulina powder, 15 parts of high-gluten flour, 7 parts of krill powder, 6 parts of mealworm powder, 5 parts of glycolic acid bacteria protein powder, 2 parts of fish oil, 2 parts of yeast extract, 1.5 parts of potassium dihydrogen phosphate, 1.4 parts of liquid lecithin, 0.5 parts of mulberry leaf powder, 0.2 parts of mung bean powder, 0.2 parts of Haematococcus pluvialis powder, 0.1 parts of yeast selenium, and 0.1 parts of fish soluble slurry.

3. The antibiotic-free feed for shrimps according to claim 1, characterized in that: The parameter indicators of the spirulina powder are: protein content is 61%-62%, fat content is less than 4%, moisture content is less than 10%, ash content is less than 10%, and iron content is less than 800 mg / kg.

4. The antibiotic-free feed for shrimps according to claim 1, characterized in that: The protein content of the ethanol clostridium protein powder is above 80%.

5. A method for preparing antibiotic-free feed according to any one of claims 1 to 4, characterized in that: The preparation method comprises the following steps: (1) crushing krill powder and yellow mealworm powder to obtain crushed krill powder and crushed yellow mealworm powder for later use; (2) mixing the crushed krill powder and the crushed mealworm powder with spirulina powder, high-gluten flour, ethanolic Clostridium protein powder, fish oil, yeast extract, potassium dihydrogen phosphate, liquid lecithin, mulberry leaf powder, mung bean powder, Haematococcus pluvialis powder, yeast selenium, and fish slurry, and mixing them evenly, and then crushing them in an ultrafine grinder to obtain a composite crushed material for use; (3) The composite crushed material is conditioned in a conditioning device to control the moisture content to 25%-30%, and then granulated in a single screw extruder to obtain granular material for later use; (4) Drying the above pellets to obtain antibiotic-free feed.

6. The preparation method according to claim 5, characterized in that: In the step (1), the crushed krill powder and the crushed mealworm powder are sieved through a 40-mesh sieve.

7. The preparation method according to claim 5, characterized in that: In the step (2), the composite crushed material is passed through an 80-mesh sieve.

8. The preparation method according to claim 5, characterized in that: The temperature entering the single screw extruder in step (3) is 100-120°C.

9. The preparation method according to claim 5, characterized in that: The drying temperature in step (4) is 90-100°C.