Micro-ecological flora balanced natural cranberry nourishing and improving causalgia and peculiar smell moisturizing and tightening essence, preparation method and application of micro-ecological flora balanced natural cranberry nourishing and improving causalgia and peculiar smell moisturizing and tightening essence

Abstract

The invention discloses a micro-ecological flora balanced natural cranberry essence capable of nourishing and improving causalgia and peculiar smell, a preparation method and application, the cranberry essence is prepared from the following raw materials in percentage by weight: 12-25% of cranberry oil, 70-85% of cyperus esculentus oil and 3-5% of pepper fruit extract. The composition can promote the growth of beneficial bacteria, inhibit the growth of harmful bacteria, maintain the balance of micro-ecological flora, improve the skin elasticity, provide a moistening effect, enhance the barrier function of the skin, protect the skin from being damaged by the external environment, reduce skin sensitivity, reduce skin discomfort, relieve pruritus, and reduce stabbing pain and burning heat sensation. The disposable hygienic product prepared from the essence can keep micro-ecological flora balance and reduce skin discomfort. The wash supplies prepared from the essence are externally smeared on the skin, so that the skin has skin care activity, and the essence has good effects of inhibiting harmful bacteria, delaying skin degenerative change and moisturizing and tightening the skin.

Description

Technical Field

[0001] The invention relates to a disposable sanitary product, in particular to an essence liquid for the disposable sanitary product, a preparation method and application thereof. Background Art

[0002] Antibacterial (or antibacterial) liquid products, wet wipes, sanitary napkins, panty liners, sanitary pants and other disposable sanitary products for women are close to the vulva skin. The female vulva is in a humid and stuffy environment for a long time, which can cause redness, swelling, pain, itching and discomfort. In addition, long-term contact with chemical substances can easily cause irritation and allergies. When the number of bacteria is too large, it can easily induce inflammation and discomfort. These problems seriously affect women's quality of life. Summary of the invention

[0003] In order to overcome the deficiencies of the prior art, the present invention provides a cranberry essence liquid capable of promoting the growth of beneficial bacteria, inhibiting the growth of harmful bacteria, and maintaining the balance of microecological flora, as well as a preparation method and use thereof.

[0004] The technical solution adopted by the present invention to solve its technical problem is: A microecological flora balanced natural cranberry essence is prepared from the following raw materials in percentage by weight: 12-25% cranberry oil, 70-85% sedge oil, and 3-5% Zanthoxylum bungeanum fruit extract.

[0005] The preparation method of the above-mentioned microecological flora balanced natural cranberry essence is: add the above-mentioned weight portions of cranberry oil, sedge oil, and prickly ash fruit extract into a blender, and stir and mix at a speed of 50-60 rpm for 15-20 minutes to obtain cranberry essence.

[0006] A disposable sanitary product comprises the above-mentioned microecological flora balanced natural cranberry essence.

[0007] A cranberry essence fiber layer for disposable sanitary products, the preparation method of which is: applying the above-mentioned microecological flora balanced natural cranberry essence onto a viscose fiber layer, drying and forming, and then cutting, pulling and winding to obtain the cranberry essence fiber layer for disposable sanitary products.

[0008] A disposable sanitary product comprises the cranberry essence fiber layer. The disposable sanitary product comprises, from top to bottom, a skin-contacting surface layer, a cranberry essence fiber layer, an absorbent resin layer, a dust-free paper layer, a release paper layer and a PE coating layer.

[0009] A microecological flora balanced washing and skin care product comprises the above-mentioned microecological flora balanced natural cranberry essence.

[0010] The invention has the beneficial effects that the components in the essence of the invention cooperate with each other, especially cranberry oil can promote the growth of beneficial bacteria, inhibit the growth of harmful bacteria, and maintain the balance of microecological flora, sedge oil can improve the elasticity of the skin and provide a moisturizing effect, enhance the barrier function of the skin, and protect the skin from damage by the external environment, and the Zanthoxylum bungeanum fruit extract can reduce skin sensitivity, reduce skin discomfort, relieve itching, and reduce stinging and burning sensations. The disposable sanitary products made of the essence can maintain the balance of microecological flora and reduce skin discomfort. The washing and care products made of the essence are applied externally on the skin to obtain skin care activity, have good inhibitory effects on harmful bacteria, restore microbial membranes, and prevent pathogenic bacteria activity, and also have antioxidant interruption of free radicals, delay skin degenerative changes, promote collagen growth, and make the skin moisturized and firm. DETAILED DESCRIPTION

[0011] A microecological flora balanced natural cranberry essence is prepared from the following raw materials in weight percentage: 12-25% cranberry oil, 70-85% sedge oil, and 3-5% prickly ash fruit extract. The preparation method of the microecological flora balanced natural cranberry essence is: add the above weight percentages of cranberry oil, sedge oil, and prickly ash fruit extract into a blender, stir and mix at a speed of 50-60 rpm for 15-20 minutes to obtain cranberry essence. In this embodiment, the cranberry oil is selected from the product model Naturex TM The selected product of cranberry virgin oil and cyperus rotundus oil is KM COSMA® cyperus rotundus oil, and the selected product of Zanthoxylum bungeanum fruit extract is Zanthalene® Zanthoxylum bungeanum fruit extract.

[0012] The microecological flora balanced natural cranberry essence of the present invention can be applied to disposable sanitary products, such as sanitary napkins, panty liners, sanitary pants, etc., and can also be applied to toiletries, such as essence, wet wipes base liquid, antibacterial lotion, and antibacterial gel. The toiletries made of the essence are applied externally on the skin to obtain skin care activity, have good functions of inhibiting harmful bacteria, restoring microbial membranes, and preventing pathogenic bacteria, and also have antioxidant effects of interrupting free radicals, delaying skin degenerative changes, promoting collagen growth, and making the skin moisturized and firm.

[0013] When used for disposable sanitary products, a cranberry essence fiber layer needs to be prepared in advance. The preparation method of the cranberry essence fiber layer is: the above-mentioned microecological flora balanced natural cranberry essence is applied to the viscose fiber layer and dried to form, and then cut, pulled and rolled to obtain the cranberry essence fiber layer for disposable sanitary products.

[0014] The disposable sanitary product using the cranberry essence fiber layer includes, from top to bottom, a skin-contacting surface layer, a cranberry essence fiber layer, an absorbent resin layer, a dust-free paper layer, a release paper layer and a PE coating layer. Except for the cranberry essence fiber layer, the rest of the structure is the same as that of the current disposable sanitary products.

[0015] Microecological flora balance natural cranberry essence This embodiment provides three reference formulas. Formula 1: 12% cranberry oil, 85% sedge oil, 3% Zanthoxylum fruit extract. Formula 2: 20% cranberry oil, 76% sedge oil, 4% Zanthoxylum fruit extract. Formula 3: 25% cranberry oil, 70% sedge oil, 5% Zanthoxylum fruit extract.

[0016] Cranberry oil comes from cranberry seeds and has main antioxidant, barrier repair, brightening and nourishing effects. Its active ingredients can inhibit the biofilm formation of pathogenic bacteria, enhance the anti-adhesion activity of in vitro and urinary bacteria, and allow pathogenic bacteria to flow away with the urine, thereby preventing the occurrence of infection. It helps maintain private area health and relieve symptoms such as private area odor and itching.

[0017] In order to form a biofilm, individual bacteria gather together and secrete sticky polysaccharides. Myricetin in cranberry oil can reduce the secretion of adhesive polysaccharides (glucan, fructan) by certain bacteria, preventing biofilm formation. Then, the maturation of biofilm depends on iron. Type A proanthocyanidins (proanthocyanidin A2) in cranberry oil are iron chelators. Under iron-restricted conditions, some bacteria can only form thinner, immature biofilms. Bacterial proliferation is limited in immature biofilms, and bacteria are more susceptible to damage, destroying the maturity of biofilms. Then, 10-hydroxy-2-decenoic acid in cranberry oil can weaken the protective matrix or cause the structure of the biofilm to dissolve, making bacteria vulnerable to attack and causing biofilm dissolution.

[0018] Antioxidant activity experiment (in vitro experiment): Culture conditions: NHEKs (normal human epidermal keratinocytes) were pre-incubated with cranberry oil for 24 hours, and oxidative stress was treated with tert-butyl peroxide solution TBP (5mM) to dynamically generate ROS (active oxygen species) for 1 hour. Product testing: Positive reference: 200μM resveratrol, containing 0.05% cranberry oil diluted in sterile water + 1% Tween 20, experimental evaluation: reactive oxygen species generation (DCFH-DA fluorescent probe), the experiment showed that cranberry oil significantly reduced the generation of ROS (active oxygen species) by 43%.

[0019] Anti-inflammatory activity experiment (in vitro experiment): Culture conditions: NHEKs were pre-incubated for 24 hours, and PMA (1ng / ml) was used for oxidative stress treatment. Product testing: Positive reference: dexamethasone 1μM, containing 0.005% cranberry oil diluted in sterile water + 1% Tween 20. Experimental evaluation: Release of IL-8 (interleukin-8) and TNF-α (α tumor necrosis factor). The experiment showed that cranberry oil showed anti-inflammatory effects, reducing IL-8 and TNF-α by 33% and 32%.

[0020] Anti-inflammatory activity In vitro test of vaginal epithelial cells: Culture conditions: EpiVaginal™ tissue is derived from normal human vaginal epithelial cells, pre-incubated with active ingredients for 24 hours, stimulated with flagellin 100ng / mL, simulating bacterial inflammation faced during bacterial vaginosis, for 24 hours, Product testing: Positive reference: Dexamethasone 1μM, containing 0.05% cranberry oil diluted in sterile water + 1% Tween 20, Experimental evaluation: IL-6 release, Experimental results show that cranberry oil shows anti-inflammatory effects, reducing IL-6 by 36%.

[0021] Anti-irritation activity experiment (in vitro test): Culture conditions: Exvivo (skin explants) were stimulated with 0.2% SDS for 40 minutes, twice a day for 4 consecutive days. The explants were rinsed twice with PBS after each stimulation. The active substance was used for treatment after each stimulation. Product testing: 0.5% cranberry oil was diluted in sterile water + 1% Tween 20. Experimental evaluation: HE staining was performed for blind evaluation of the stimulation. The experiment showed that it has the activity of resisting the irritation caused by the use of daily detergents.

[0022] Gardnerella and Candida albicans microecological flora balance experiment: Culture conditions: Gardnerella (from vaginal secretions) and Candida albicans (from vaginal secretions), Gardnerella incubated in an anaerobic environment for 48 hours, Candida albicans incubated in an aerobic environment for 18 hours, pH 5.5, MIC determined according to ISO20776-1. Product testing: strain control: purified water + bacterial suspension, cranberry oil (0.001%-0.5%) + sterile water containing strains + 1% Tween 20, experimental evaluation: absorbance at 600nm was measured, and the experiment showed that Candida albicans and Gardnerella were reduced by 99% and 96% respectively.

[0023] Lactobacillus jensenii microecological flora balance experiment: Culture conditions: Lactobacillus jensenii (from vaginal secretions), incubated under anaerobic conditions at pH 6 for 42 hours, MIC determined according to ISO20776-1, product testing: strain control: pure water + bacterial suspension, cranberry oil (0.001%-0.5%) + sterile water containing strains + 1% Tween 20, experimental evaluation: determination of absorbance at 600nm and pH determination, the experiment showed that: at a concentration of 0.5%, cranberry oil can significantly increase Lactobacillus jensenii by 66 times and restore the pH value.

[0024] Experiment on the balance of microecological flora of Lactobacillus jensenii and Candida albicans: Co-culture conditions: Strains: Lactobacillus jensenii (from vaginal secretions) and Candida albicans (from the vagina), cultured under anaerobic conditions of pH 6 for 18 hours, product testing: strain control: purified water + bacterial suspension, cranberry oil (0.5%) + sterile water containing strains + 1% Tween 20, experimental evaluation: bacterial count after ultrafiltration, pH measurement, the experiment showed that cranberry oil inhibited the excessive proliferation of pathogenic bacteria, was conducive to the growth of symbiotic bacteria, and lowered the pH value to balance the vaginal microbiome.

[0025] Cyperus oleiferus oil is an extract from the roots of Cyperus oleiferus. It is rich in unsaturated fatty acids, including 74% oleic acid, 9% linoleic acid, and 0.2% linolenic acid. It is also rich in vitamins B, E, folic acid, etc. It does not contain cholesterol and is highly antioxidant. Cyperus oleiferus oil is rich in monounsaturated fatty acids, with a content of more than 57%. Since monounsaturated fatty acids have fewer saturated bonds than polyunsaturated fatty acids, their properties are relatively stable and not easily oxidized. Cyperus oleiferus oil is rich in vitamin E and linoleic acid, which can be used to improve skin elasticity and provide moisturizing effects, enhance skin gloss, strengthen skin barrier function, and protect skin from damage by the external environment.

[0026] The standard active ingredient in Zanthoxylum bungeanum fruit extract is α-hydroxysanshool, an alkyl amide. When used in small doses, it can interact with receptors to eliminate skin discomfort (slight dulling of sensory neurons). When used in large doses, it can induce muscle relaxation and reduce the appearance of skin wrinkles (nerve / muscle signal interruption, a botulinum toxin-like effect). When used in appropriate amounts, it can reduce skin sensitivity, reduce skin discomfort, relieve itching, and reduce tingling and burning sensations.

[0027] Experiment on inhibiting muscle cell contraction: Muscle cells and motor neurons were co-cultured, and Zanthoxylum bungerotoxin (0.01%) and positive control (1μM α-bungarotoxin) were added respectively. The contraction frequency was evaluated by video imaging (recording for 30 seconds every 15 minutes). The experimental results showed that at a dose of 0.01%, muscle contraction could be immediately and significantly inhibited, and the contraction frequency decreased by 88% (p<0.0001).

[0028] Neurotransmitter depletion experiment: Zanthoxylum bungeanum extract was placed in contact with the neuromuscular junction to evaluate its activity on nerve / impulse transmission. The experimental results showed that Zanthoxylum bungeanum extract had a strong and short-lived effect on neuromuscular transmission, rapidly leading to neurotransmitter depletion and preventing muscle contraction.

[0029] The prepared personal care products (essence, wet wipes base, antibacterial lotion, antibacterial gel, sanitary napkins, panty liners, feminine hygiene pants, tampons, etc.) regulate the balance of skin colonies, inhibit harmful bacteria, restore microbial membranes, and prevent pathogens from invading the skin, so that the barrier function of microorganisms can be maintained and strengthened. It also takes into account moisturizing, locking in moisture, neutralizing free radicals, maintaining the skin's water permeability barrier, and reducing skin inflammation and itching; it significantly improves vaginal dryness, promotes vaginal cell regeneration, restores vaginal elasticity, makes the skin soft and smooth, and can also replenish moisture, increase elasticity, and make it hydrated.

[0030] The bacterial flora balance cranberry fiber layer is responsible for promoting the growth of beneficial bacteria and inhibiting the growth of harmful bacteria to maintain the balance of microecological bacterial flora. The absorbent resin, release paper and dust-free paper are responsible for absorbing body fluids and preventing leakage, overcoming the shortcomings and deficiencies of the existing technology.

[0031] When applied externally on the skin, it has skin care activity, has excellent effects in inhibiting harmful bacteria, restoring microbial membranes, and preventing pathogenic bacteria. It also has antioxidant effects in interrupting free radicals, delaying skin degenerative changes, promoting collagen growth, and making the skin hydrated, firm and elastic.

[0032] After disposable sanitary napkins were made from Formula 1, Formula 2 and Formula 3 and mixed, they were randomly distributed to women aged 25-50 of the applicant company for testing. After three months of continuous testing, the women generally reported that they were effective in terms of refreshing the vulva, relieving itching and menstrual discomfort.

[0033] The components of the essence of the present invention cooperate with each other, especially cranberry oil can promote the growth of beneficial bacteria, inhibit the growth of harmful bacteria, maintain the balance of microecological flora, sedge oil can improve the elasticity of the skin and provide a moisturizing effect, enhance the barrier function of the skin, and protect the skin from damage by the external environment, and the Zanthoxylum fruit extract can reduce skin sensitivity, reduce skin discomfort, relieve itching, and reduce stinging and burning sensations. The disposable sanitary products made of the essence can maintain the balance of microecological flora and reduce skin discomfort.

[0034] The above implementation modes cannot limit the protection scope of the present invention. The equivalent modifications and changes made by those skilled in the art without departing from the overall concept of the present invention are still within the scope of the present invention.

Claims

1. A microecological flora balanced natural cranberry essence, characterized in that The invention is prepared from the following raw materials in percentage by weight: 12-25% cranberry oil, 70-85% sedge oil and 3-5% Zanthoxylum bungeanum fruit extract.

2. The microecological flora balanced natural cranberry essence according to claim 1, characterized in that The invention is prepared from the following raw materials in percentage by weight: 12% cranberry oil, 85% sedge oil and 3% Zanthoxylum bungeanum fruit extract.

3. The microecological flora balanced natural cranberry essence according to claim 1, characterized in that The invention is prepared from the following raw materials in percentage by weight: 20% cranberry oil, 76% sedge oil and 4% Zanthoxylum bungeanum fruit extract.

4. The microecological flora balanced natural cranberry essence according to claim 1, characterized in that The product is made from the following raw materials in percentage by weight: 25% cranberry oil, 70% sedge oil, and 5% Zanthoxylum bungeanum fruit extract.

5. A method for preparing a natural cranberry essence with balanced microecological flora as described in any one of 1 to 4, characterized in that The above-mentioned parts by weight of cranberry oil, sedge oil and Zanthoxylum bungeanum fruit extract are added into a blender, and stirred and mixed at a rotation speed of 50-60 rpm for 15-20 minutes to obtain cranberry essence.

6. A disposable sanitary product, characterized in that The invention comprises the cranberry essence as claimed in any one of claims 1 to 4.

7. A cranberry essence fiber layer for disposable sanitary products, characterized in that The preparation method is as follows: the cranberry essence liquid described in any one of claims 1 to 4 is coated on a viscose fiber layer, dried and formed, and then cut, pulled and rolled to obtain a cranberry essence liquid fiber layer for disposable sanitary products.

8. A disposable sanitary product, characterized in that The disposable sanitary product comprises the cranberry essence fiber layer as claimed in claim 7, and comprises, from top to bottom, a skin-contacting surface layer, a cranberry essence fiber layer, an absorbent resin layer, a dust-free paper layer, a release paper layer and a PE coating layer.

9. A microecological flora balanced toiletries, characterized in that The invention comprises the cranberry essence as claimed in any one of claims 1 to 4.