Amino acid phagostimulant for juvenile mollusca and application thereof

Abstract

The invention discloses an amino acid phagostimulant for juvenile helichthys nobilis, which mainly comprises aspartic acid, glutamic acid, alanine, glycine, proline and methionine in a content ratio of (4-5): (5-6): (4-5): 3: 2: 1, and the six amino acids are reasonably compounded, so that the phagostimulant for the juvenile helichthys nobilis achieves the best phagostimulant effect on the juvenile helichthys nobilis. The invention also discloses a juvenile mollusca lanuginosa feed based on the amino acid phagostimulant, the juvenile mollusca lanuginosa feed comprises a basal feed and the phagostimulant, and the mass percentage content of the phagostimulant in the juvenile mollusca lanuginosa feed is 0.79-0.96%. The amino acid phagostimulant is added into the basal feed, so that the food-calling fresh fruit of the young mollusca is obviously higher than that of traditional baits, the increase rate of the body weight and the body length of the young mollusca is higher, the survival rate is maximum, and the application prospect is very good.

Description

Technical Field

[0001] The invention relates to an amino acid attractant for young snails and a preparation method and application thereof, belonging to the technical field of attractants for young snails and feeds thereof. Background Art

[0002] Naptunea cumingi crosse belongs to the phylum Mollusca, class Gastropoda, order Neogastropoda, family Buccinida, genus Neptunea. It is mainly distributed in the Yellow Sea and Bohai Sea in my country and the northern waters of Japan and North Korea. It is a large carnivorous snail in temperate marine waters. Its meat is delicious and nutritious, and it has high edible and economic value. At present, the acquisition of Naptunea cumingi crosse mainly depends on wild fishing. However, overfishing has led to a sharp decline in its natural population, posing a threat to the ecological balance. In order to alleviate the fishing pressure of Naptunea cumingi resources and meet people's consumption demand for Naptunea cumingi crosse, it is particularly urgent to develop artificial breeding technology for Naptunea cumingi crosse.

[0003] At present, scholars' research on conchs is mainly focused on behavior, reproduction and other aspects. Although the trial of hanging culture technology for adult conchs in the sea area has been successful and achieved good economic benefits, the acquisition of juvenile conchs comes from natural seedlings in the sea area, which is far from meeting the needs of large-scale culture. The existing artificial culture technology is limited by the lack of understanding of the nutritional needs of conchs, and a breakthrough is urgently needed.

[0004] Unlike other snails, the fragrant snail does not go through the planktonic and metamorphosis stages, but develops directly from the fertilized egg to the juvenile snail. The embryo is supplied with nutrition by the nutrient eggs in the egg capsule. The newly hatched juvenile snail can feed on benthic diatoms, but soon becomes carnivorous, and its main food objects are benthic bivalve molluscs and rotten fish. Juvenile snails cannot easily catch shellfish prey and cannot open the shells independently. They need to drill holes in the shells to ingest food. Therefore, the survival rate of juvenile fragrant snails in the natural environment is low. In artificial breeding, if the soft parts of shellfish or miscellaneous fish are directly fed as bait to feed juvenile fragrant snails, the cost is high and the leftover bait that is not eaten in time is also very easy to spoil, causing water pollution, and it is easy to contain pathogens that lead to the death of young snails and waste of fishery resources. In addition, the sufficient supply of bait cannot be guaranteed during the fishing moratorium.

[0005] In order to solve the above problems, there have been reports on artificial feeds for snails. For example:

[0006] The Chinese patent publication number CN 102550846 A is "a compound feed for snails containing ginkgo powder". It mainly adds a small amount of ginkgo powder and shrimp meat powder to the basic feed, and uses a certain amount of soybean meal and seaweed powder to partially or completely replace fish meal. The purpose of the invention is to effectively solve the feed problem of artificial breeding of snails, enable snails to absorb quickly and promote their growth; regulate their metabolic level, improve the snail's resistance to stress, reduce the pollution of bait to the environment, improve breeding safety, and increase production and survival. However, this feed still has the problems of low utilization rate and slow growth rate of snails.

[0007] The applicant also applied for an invention patent on September 13, 2023, "An artificial feed for snails containing natural attractants" (application number 202311177020.1, priority 202310773200.X), which aims to improve the feeding behavior of snails and increase the food intake of snail larvae, and promote the rapid growth of snails. This feed replaces existing protein sources such as shrimp meal, soybean meal and fish meal with chicken meal, Methylcoccus capsularis protein powder, Ethanol Clostridium protein powder and Candida utilis powder, and adds Microcystis powder, Schizochytrium powder, soybean oil, chitosan oligosaccharides, microcrystalline cellulose, complex vitamins, complex minerals and natural attractants. In this invention application: the natural attractant is a mixture of the enzymatic hydrolysis product of river clams and the carbon dioxide supercritical extraction product of Manila clams in a certain proportion. This artificial feed containing natural attractants can significantly improve the quality of conch meat, save breeding costs, reduce water pollution, and improve breeding benefits. However, the use of the above-mentioned attractants still has the following problems:

[0008] (1) Cost issues: The production process of river mussel protein hydrolysate and Philippine clam carbon dioxide supercritical extraction products may involve high technology and costs, which may lead to an increase in the price of the final product;

[0009] (2) Technical requirements: The enzymatic hydrolysis and supercritical extraction involved in the preparation process are technically demanding and may require specialized equipment and technicians, which may limit its application in small-scale farms;

[0010] (3) Environmental impact: If the waste and by-products generated during the production of the attractant are not properly handled, they may also have a certain impact on the environment;

[0011] (4) Consistency of effect: Since the content of bioactive substances in river clams and Manila clams may vary depending on factors such as season and growth environment, this may affect the consistency of the attractant effect.

[0012] Amino acids are recognized as one of the effective attractants in the aquatic industry. They can stimulate the taste and smell of aquatic animals to attract food. Therefore, they have been widely used as attractants in aquatic animals, but currently, their application scope is mainly concentrated in fish. Different amino acids, due to differences in molecular structure, cause fish olfactory and taste receptors to have completely different sensitivity and recognition. Therefore, different amino acids have different attractant effects on different fish. For example, adding arginine and glutamic acid to carp feed has the best effect, and adding alanine and methionine also promotes feeding. Adding cystine to grass carp feed has a significant effect, and adding arginine, alanine, and methionine also has an effect. This shows that different amino acids have different synergistic effects after compounding, and the attractant effect is also very different. In the study of Chang Qing, Liang Mengqing, etc., it was found that L-alanine and glycine can well promote the feeding of red-finned pufferfish (Takifugu rubripes), but this is related to the added content. Qin Weilun's research found that when the amount of L-alanine and glycine added was 0.5%, the dark-striped pufferfish (Takifugu fasciatus) significantly increased its food intake, while the effect was not obvious when the amount was 1%. Huang Jie et al. studied the luring effect of different concentrations of attractants on field snails (Procambarus clarkii) and found that low concentration (0.2%) of alanine had the best effect among all the test concentrations. The reason may be that field snails have a certain repulsion to strong odors. Zhou Shuangnan et al. also reached a similar conclusion in their research: when the amount of glycine added to the feed was 0.2%, the food intake of Hemifusus tuba Gmelin was the largest and the weight gain effect was the best. The above studies further show that different amino acids and their combinations have completely different luring effects on aquatic animals. Therefore, the research on amino acid attractants for young snails has become the main research focus of the applicant. Summary of the invention

[0013] In order to overcome the deficiencies of the prior art, the object of the present invention is to provide an amino acid attractant which can effectively increase the feeding rate of young snails and thus affect the growth performance of young snails.

[0014] In order to achieve the above object, the present invention adopts the following technical solution:

[0015] The amino acid attractant for juvenile snails is formed by mixing aspartic acid, glutamic acid, alanine, glycine, proline and methionine in a mass ratio of (4-5):(5-6):(4-5):3:2:1.

[0016] Furthermore, the optimal mass ratio of the amino acid components of the above-mentioned attractant: aspartic acid, glutamic acid, alanine, glycine, proline and methionine is 4:6:5:3:2:1.

[0017] The amino acid attractant based on the above components and proportions is mainly used in the preparation of feed for juvenile snails.

[0018] The present invention also provides a feed based on an amino acid attractant for young snails, comprising a basic feed for young snails and the attractant, wherein the mass percentage of the attractant in the feed is 0.79-0.96%.

[0019] The basic feed for young snails includes the following components: fish meal, fish oil, flour, mineral premix and vitamin premix. The mass ratio of fish meal, fish oil, flour, mineral premix and vitamin premix is: (80-90): (3-5): (8-10): (0.5-1): (0.5-1).

[0020] Furthermore, the present invention also provides a method for preparing a feed based on an amino acid attractant for young snails of Conchocera odorata, which mainly comprises the following steps:

[0021] S1 Raw material preparation: first prepare aspartic acid, glutamic acid, alanine, glycine, proline and methionine according to the mass ratio, and then prepare fish meal, fish oil, flour, mineral premix and vitamin premix according to the mass ratio;

[0022] S2 Hybrid:

[0023] (1) Aspartic acid, glutamic acid, alanine, glycine, proline and methionine are poured into container 1 in order from least to most and mixed thoroughly to form an amino acid attractant.

[0024] (2) Pour fish meal, flour, mineral premix and vitamin premix into container 2 in order from least to most and mix thoroughly.

[0025] (3) Add the amino acid attractant into container 2 and mix well.

[0026] (4) Finally, add fish oil to container 2, knead until the fish oil is completely absorbed, then add an appropriate amount of clean water to knead the feed into a ball;

[0027] S3 granulation: the feed in the form of a pellet in step S2 is made into a granular feed suitable for young snails to eat by the snails through a pelleting machine;

[0028] S4 Drying: After the pelletized feed is dried in the sun until it is half dry, it is placed in a 60°C oven to dry. After cooling to room temperature, it is bagged and sealed and stored in a -20°C refrigerator.

[0029] More preferably, the particle size of the fish meal in step S1 is sieved through an 80-mesh sieve, and the particle size of the granular feed in step S3 is less than 2 mm.

[0030] The invention is beneficial in that:

[0031] (1) Enhanced feeding effect: The present invention forms a synergistic feeding attractant through a specific amino acid combination and ratio, and compounded into feed, which can act on multiple receptors at the same time, thereby generating a stronger feeding signal, effectively stimulating the feeding response of young snails, and enhancing the feeding effect;

[0032] (2) Improving growth rate: The specific amino acid compound of the present invention can effectively supplement the deficiency of essential amino acids in feed, improve the nutritional value of feed, and thus promote the growth and health of young snails;

[0033] (3) Improved digestive enzyme activity: The attractant of the present invention effectively enhances the secretion and activity of the digestive enzyme of the young snails through the synergistic effect of specific amino acids, thereby greatly improving the digestion and absorption efficiency of the young snails during the feeding process;

[0034] (4) Improved bait utilization: The young snails that eat the feed of the present invention have excellent performance in lipase activity and bait coefficient, showing good utilization, reducing dependence on natural bait resources and reducing the adverse effects of residual bait on the environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a schematic diagram of the amount of food attracted to juvenile snails of the fragrant snail by different baits;

[0036] Figure 2 This is a graph showing the weight changes of young snails fed with different baits;

[0037] Figure 3 The shell height variation diagram of three groups of juvenile snails of the fragrant snail;

[0038] Figure 4 The shell width variation diagram of three groups of juvenile snails of the conch;

[0039] Figure 5 Schematic diagram of digestive enzyme activity of three groups of juvenile snails of Ophiopogon fuscipenser. DETAILED DESCRIPTION

[0040] The following is a clear and complete introduction to the technical solutions in the embodiments of the present invention in combination with the accompanying drawings and specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] Unless otherwise stated, all tests and measurements were carried out under “normal conditions”. The term “normal conditions” means 20°C, 1013 hPa and 50% relative humidity.

[0042] For the purposes of the present disclosure, the term "free from" means that the proportion of the corresponding substance is less than 0.05% by weight. This ensures that "free from" according to the present invention does not include inclusions and impurities containing these substances.

[0043] Unless otherwise specified, the content values ​​of each component in the formula table of this embodiment are all mass percentages (mass percentages) in the entire formula system, and the balance is water.

[0044] Prepare artificial basic feed: the ingredients are as shown in Table 1 (fish meal is used as protein source, fish oil is used as fat source, and flour is used as adhesive):

[0045] Table 1 Ingredients of artificial basic feed

[0046]

[0047] In Table 1: The nutritional components of artificial basic feed mainly include: crude protein, crude fat and ash.

[0048] Prepare an artificial compound feed of attractant type: the ingredients are shown in Table 2 (the feed based on the amino acid attractant for young snails of the present invention):

[0049] Table 2 Ingredients of artificial compound feed in attractant dosage form

[0050]

[0051] The preparation method of the artificial compound feed of the attractant type is as follows:

[0052] S1 Raw material preparation: first, prepare aspartic acid, glutamic acid, alanine, glycine, proline and methionine according to the mass ratio, and then prepare fish meal, fish oil, flour, mineral premix and vitamin premix with a particle size of 80 mesh sieve according to the mass ratio;

[0053] S2 Hybrid:

[0054] (1) Pour methionine, proline, glycine, aspartic acid, alanine, and glutamic acid into container 1 in sequence and mix thoroughly to form an amino acid attractant.

[0055] (2) Pour the mineral premix, vitamin premix, flour and fish meal into container 2 in sequence and mix thoroughly.

[0056] (3) Add the amino acid attractant into container 2 and mix well.

[0057] (4) Finally, add fish oil to container 2, knead until the fish oil is completely absorbed, then add an appropriate amount of clean water to knead the feed into a ball;

[0058] S3 granulation: the feed in the form of agglomerates in step S2 is made into granular feed with a particle size of less than 2 mm and suitable for young snails to eat by the juvenile snails of the European snail family;

[0059] S4 Drying: After the pelletized feed is half dried in the sun, it is placed in a 60°C oven to dry. After cooling to room temperature, it is bagged and sealed in a -20°C refrigerator for storage and use.

[0060] Example 1: Test on the feeding effect of fresh soft parts of Sinonovacula constricta and Yesso scallop after shelling (natural bait), artificial basic feed and artificial compound feed in attractant form on juvenile snails of Cirrhilabrus constrictus.

[0061] The juvenile snails used in the experiment were from the same batch of seedlings and were temporarily raised in a water tank. The natural seawater temperature used in the experiment was 10℃ and the pH value was 8.0.

[0062] The experiment was conducted in the marine aquaculture laboratory of Liaoning Academy of Marine Fisheries Sciences from mid-November to the end of December 2023. 40 young snails were randomly selected and placed in the center of another tank for feeding experiments. Equal amounts of fresh soft parts of razor clams and shelled scallops, as well as artificial basic feed and artificial compound feed in the form of attractants, were placed in the four corners of the tank. The number of young snails attracted by each bait was recorded using a time-lapse camera at the same time every day. The experiment was repeated three times and the average value was selected.

[0063] Figure 1 Schematic diagram of the amount of food attracted to juvenile snails of the fragrant snails by different baits.

[0064] like Figure 1 As shown: Young snails prefer to eat artificial compound feed in the form of attractant, followed by Yesso scallops, then Sinonovacula constricta, and finally artificial basic feed.

[0065] Example 2: Experiment on the effects of shelled fresh Sinonovacula constricta, artificial basic feed and artificial compound feed in attractant form on the growth performance of juvenile snails.

[0066] The experiment was set up in three groups, each with three parallels. Each group was placed with 40 young snails weighing (0.80±0.10)g, with a shell height of (18.08±0.09)mm and a shell width of (11.15±0.08)mm. They were fed with shelled fresh razor clams, artificial basic feed and artificial compound feed in the form of attractants. During the experiment, they were fed with 1.5% of the body weight of the young snails. The glass cylinders used in the experiment were 30cm cubes, and water was added to 2 / 3 of the glass cylinders. Each glass cylinder was equipped with an oxygen-enhancing stone and aerated for 24 hours to keep the dissolved oxygen content in the water >6mg / L. All nine groups of glass cylinders were placed in a large water tank, and the water tank was filled with water to keep the water surface inside and outside the glass tank consistent. Four heating rods were placed equidistantly in the water tank to ensure that the water temperature was always controlled at 15℃ during the experiment. The seawater used in the experiment was natural sand-filtered seawater. Water was stored and aerated in a PVC bucket every day. The heating rod was turned on 1h before changing the water to raise the water temperature to 15℃. The water was changed at 15:00 every day and the leftover bait and feces were collected in a net bag by siphoning, rinsed with distilled water for desalination, and dried in an oven at 60°C to constant weight to calculate the daily food intake. The young snails were gently removed every week, and their weight, shell height and shell width were measured after the surface water was absorbed. The experiment lasted for 6 weeks.

[0067] (1) Growth performance

[0068] After the experiment, the specific growth rate, feed conversion rate, survival rate, digestive enzyme activity and nutritional components of each group of juvenile snails were measured. The calculation formulas for specific growth rate, feed conversion rate and survival rate are as follows:

[0069] Specific growth rate SCR = 100% [ln (DW2) - ln (DW1)] / T;

[0070] Feed coefficient FAR = C / (M2-M1) × 100%;

[0071] Survival rate SR = (F2 / F1) x 100%.

[0072] In the formula, DW1 represents the initial dry weight of juvenile snails, g; DW2 represents the final dry weight of juvenile snails, g; C represents the total food intake, g; M1 represents the initial wet weight of juvenile snails, g; M2 represents the final wet weight of juvenile snails, g; F1 represents the number of juvenile snails at the beginning of the experiment; F2 represents the number of juvenile snails at the end of the experiment; T represents the experimental time, d.

[0073] (2) Digestive enzyme activity

[0074] The shells of young snails were gently broken and the snail meat was transferred to an ice tray. The intestinal tissue was separated and placed in a centrifuge tube. Each group was mixed. The intestinal samples of young snails in each experimental group were mixed with saline at a ratio of 1:9. The centrifuge was set at 4°C and 2500r / min. After centrifugation for 10 minutes, the supernatant was obtained and the activities of pepsin, lipase and amylase were measured respectively. The kits used are shown in Table 3. The specific determination methods were performed according to the instructions of the kits.

[0075] Table 3 Test reagents

[0076]

[0077] (3) Biochemical indicators

[0078] The total nitrogen content was determined by the Kjeldahl method, and the crude protein percentage was calculated as %N×6.25. The total fat concentration was determined by Soxhlet extraction using petroleum ether as the solvent carrier, and the crude fat content was calculated by the weight method. The sample was roasted to 550°C for 6 hours, and the ash content was determined. The determination of crude protein and crude fat was carried out in accordance with the national standards "Determination of crude protein in feed" (GB / T 6432-2018) and "Determination of crude fat in feed" (GB / T 6433-2006).

[0079] Data processing: The experimental data were expressed as mean ± standard deviation (mean ± SD). SPSS23.0 software was used to perform one-way ANOVA on the growth performance, digestive enzyme activity and nutritional components of juvenile snails. If there were significant differences, Duncan's multiple comparisons were used (the significance level was 0.05).

[0080] The growth performance of juvenile snails of the fragrant snail is shown in Table 4.

[0081] Table 4 Growth performance of juvenile snails in each group

[0082]

[0083] As shown in Table 4, the specific growth rate of the young snails in the group fed with the artificial compound feed with attractant was significantly higher than that in the basic feed group and the Sinon clam group (P<0.05), while the specific growth rate of the young snails in the Sinon clam group also showed a significant advantage over the basic feed group (P<0.05). The young snails in the group fed with the artificial compound feed with attractant had the lowest feed conversion rate and the highest feed utilization rate. Statistical analysis showed that it was significantly higher than that in the artificial basic feed group (P<0.05), but there was no significant difference compared with the Sinon clam group. During the experiment, young snails in the three groups died, but there was no significant difference in survival rate among the three groups.

[0084] Figure 2 This is a graph showing the weight changes of juvenile snails of the fragrant snail fed with different baits.

[0085] like Figure 2 As shown in the results, the weight of the young snails in each group showed an increasing trend during the experiment. In the first two weeks of breeding, except for the basic feed group, the weight of the young snails in the other two groups increased rapidly, and there was no significant difference between the two groups. From the third week, the weight growth of the young snails in the Sinonovacula constricta group began to slow down, while the weight of the young snails in the attractant type artificial feed group continued to grow rapidly. By the end of the experiment, the young snails in the attractant type artificial feed group gained 48.1%, the young snails in the Sinonovacula constricta group gained 29.6%, and the young snails in the basic feed group gained 9.9%. The final weight of the young snails in the three groups showed significant differences (P<0.05).

[0086] Figure 3 This is a graph showing the changes in shell height of three groups of juvenile snails.

[0087] like Figure 3 As shown in the figure, the shell height of the young snails in each group showed an increasing trend during the experiment. In the first two weeks of breeding, except for the basic feed group, the shell height of the young snails in the other two groups did not change much. In the third week, significant differences began to appear (P<0.05), and the shell height growth of the young snails in the Sinon clam group began to slow down. After the experiment, the shell height of the young snails in the three groups showed significant differences, among which the shell height of the young snails in the attractant type artificial feed group increased by 14.38%, the shell height of the young snails in the basic feed group increased by 4.76%, and the shell height of the young snails in the Sinon clam group increased by 10.83%.

[0088] Figure 4 This is a chart showing the changes in shell width of three groups of juvenile snails of the species Moraxellana.

[0089] like Figure 4 As shown: The shell width of the young snails in each group showed an increasing trend during the experiment. In the first three weeks of breeding, except for the basic feed group, the shell width of the other two groups was not much different. In the fourth week, significant differences began to appear among the groups. As the experiment progressed, the group with artificial compound feed with attractant showed a significant growth advantage. After the experiment, the shell width of the young snails in the group with artificial compound feed with attractant was significantly higher than that in the artificial feed group and the Sinon clam group (P<0.05). The shell width of the young snails in the group with artificial compound feed with attractant increased by 14.90%, the shell width of the young snails in the group with basic feed increased by 6.82%, and the shell width of the young snails in the group with Sinon clam increased by 8.62%.

[0090] Figure 5 Schematic diagram of digestive enzyme activity of three groups of juvenile snails of Ophiopogon fuscipenser.

[0091] like Figure 5As shown: After the experiment, the pepsin activity of young snails in the Sinonovacula constricta group and the artificial compound feed group with attractant dosage was not significantly different, but both were higher than the artificial feed group (P<0.05). The lipase activity of young snails fed with artificial compound feed with attractant dosage was significantly higher than that of the other two groups (P<0.05). The amylase activity of young snails in the three groups was low and there was no significant difference between the groups.

[0092] The above experiments further verify that the addition of amino acid attractants in the artificial feed of the present invention can promote the feeding of young snails, thereby promoting their growth, and the amino acid content in the formula is rich, especially the flavor amino acid, which can effectively promote or increase the excitability of young snails to feed, thereby achieving the purpose of attracting feeding. Feeding the attractant feed of the present invention can effectively improve the conversion rate of feed and the growth rate and survival rate of young snails, and the weight gain effect is also more prominent.

[0093] Example 3: Test on the feeding effect of feeds prepared with different amino acid combinations on juvenile snails of Cirrhilabrus odoratus.

[0094] The test method is the same as that in Example 1. Three groups are set for each formula, i.e., the addition amount of the amino acid attractant in the feed is 0.79%, 0.84% ​​and 0.96% respectively, and the attractant effect is taken as the average value, wherein the English abbreviations of each amino acid are: aspartic acid Asp, glutamic acid Glu, alanine Ala, glycine Gly, proline Pro, methionine Met, tyrosine Tyr. The attractant test results are shown in Table 5.

[0095] Table 5 Feeding effect of feeds with different amino acid combinations under the same addition range

[0096]

[0097] It can be seen from Table 5 that within the range of 0.79% to 0.96% of amino acid addition, the combination of aspartic acid, glutamic acid, alanine, glycine, proline and methionine has the best feeding effect.

[0098] The feeding effects of feeds with the same amino acid combination under different addition ranges are shown in Table 6. In each amino acid addition range, two end values ​​and one middle value are taken, and the feeding effect is finally averaged.

[0099] Table 6 Feeding effect of feeds with the same amino acid combination under different addition ranges

[0100]

[0101] With respect to the formula combination of the amino acid attractant of the present invention, within different addition content ranges, the best attractant effect is achieved within the range of 0.79%-0.96%.

[0102] For the same addition range, the same amino acid combination, and different ratios of amino acids, the attractant effect is shown in Table 7. In this experiment, similarly, at each amino acid ratio, the amount of amino acid attractant added to the feed was set in three groups, that is, the amount of amino acid attractant added to the feed was 0.79%, 0.84% ​​and 0.96%, respectively, and the attractant effect took the average value.

[0103] Table 7 The feeding effects of the same amino acid combination and different ratios of amino acids under the same addition range

[0104]

[0105]

[0106] In summary: the selection of components of amino acid attractants, the ratio between the components and the amount added in the feed all affect the final attractant effect.

[0107] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention, and the scope of the present invention is defined by the attached claims and their equivalents.

Claims

1. An amino acid phagocytic agent for young snails of Conch, characterized in that: The attractant is formed by mixing aspartic acid, glutamic acid, alanine, glycine, proline and methionine in a mass ratio of (4-5):(5-6):(4-5):3:2:

1.

2. The amino acid phagocytic attractant for young snails of claim 1, wherein The attractant is formed by mixing aspartic acid, glutamic acid, alanine, glycine, proline and methionine in a mass ratio of 4:6:5:3:2:

1.

3. Use of the amino acid attractant for juvenile snails of claim 1 or 2 in preparing feed.

4. A feed based on the amino acid attractant for young snails of claim 1 or 2, characterized in that: The invention comprises a basic feed and an attractant for young snails of Conch shells, wherein the mass percentage of the attractant in the feed is 0.79-0.96%.

5. The feed according to claim 4, characterized in that The basic feed for juvenile snails of Conch shells comprises the following components: fish meal, fish oil, flour, mineral premix and vitamin premix.

6. The feed according to claim 5, characterized in that The mass ratio of the fish meal, fish oil, flour, mineral premix and vitamin premix is: (80-90): (3-5): (8-10): (0.5-1): (0.5-1).

7. The method for preparing the feed according to any one of claims 4 to 6, characterized in that: The steps include: S1 Raw material preparation: first prepare aspartic acid, glutamic acid, alanine, glycine, proline and methionine according to the mass ratio, and then prepare fish meal, fish oil, flour, mineral premix and vitamin premix according to the mass ratio; S2 Hybrid: (1) Aspartic acid, glutamic acid, alanine, glycine, proline and methionine are poured into container 1 in order from least to most and mixed thoroughly to form an amino acid attractant. (2) Pour fish meal, flour, mineral premix and vitamin premix into container 2 in order from least to most and mix thoroughly. (3) Add the amino acid attractant into container 2 and mix well. (4) Finally, add fish oil to container 2, knead until the fish oil is completely absorbed, then add an appropriate amount of clean water to knead the feed into a ball; S3 granulation: the pelletized feed in step S2 is made into a granular feed suitable for young snails to eat by a granulator; S4 Drying: After the pelletized feed is dried in the sun until it is half dry, it is placed in a 60°C oven to dry. After cooling to room temperature, it is bagged and sealed and stored in a -20°C refrigerator.

8. The preparation method according to claim 7, characterized in that: The particle size of the fish meal in step S1 is sieved through an 80-mesh sieve.

9. The preparation method according to claim 7, characterized in that: The particle size of the granular feed is less than 2 mm.

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