Solenopsis invicta bait and preparation method thereof

Abstract

The invention discloses red imported fire ant bait and a preparation method thereof. The invention relates to a red imported fire ant bait, which is prepared from the following components in percentage by mass: 56.76 percent to 61.99 percent of corn flour, 16.57 percent to 18.85 percent of dried meat floss, 16.50 percent to 18.90 percent of peanut oil, 3.08 percent to 4.22 percent of milk powder, 0.01 percent to 0.10 percent of ethyl butyrate, 1.34 percent to 1.70 percent of potassium sorbate and 0.01 percent to 0.50 percent of insecticide. Compared with the prior art, the solenopsis invicta bait has the following advantages that on the basis of background baits (corn flour, dried meat floss, peanut oil and milk powder), potassium sorbate keeps the freshness of the baits, a trace amount of ethyl butyrate is added, the appetite of solenopsis invicta is further stimulated, the palatability of the baits to the solenopsis invicta is improved, and the service life of the solenopsis invicta bait is prolonged. Particles with the average particle size of 1.5 mm are prepared through a pelletizer and are suitable for carrying of the solenopsis invicta, so that the attraction of the bait to the solenopsis invicta is further enhanced.

Description

Technical field:

[0001] The invention relates to the technical field of red fire ant baits, and in particular to a red fire ant bait and a preparation method thereof. Background technology:

[0002] There are many registered red fire ant baits on the market. The insecticidal active ingredients are similar, but the insecticidal effects vary greatly. The difference lies in the different palatability of the bait formula. How to improve the palatability of the bait is the key to formula research and development. Summary of the invention:

[0003] The invention solves the problems existing in the prior art and provides a red fire ant bait and a preparation method thereof.

[0004] The invention prepares meat floss, corn flour, peanut oil and milk powder in reasonable proportions, uses a preservative (potassium sorbate) to keep the freshness of bait, adds an attractant (ethyl butyrate) to further stimulate the appetite of red fire ants and improve the palatability of the bait to the red fire ants, and granulates the bait with a granulator to obtain a particle size suitable for the red fire ants to carry, thereby further enhancing the attraction of the bait to the red fire ants. The red fire ant bait prepared by adding an insecticide (dinotefuran) can efficiently kill the red fire ants, thereby achieving the purpose of the invention.

[0005] The first purpose of the present invention is to provide a red fire ant bait, which is composed of 56.76% to 61.99% corn flour, 16.57% to 18.85% meat floss, 16.50% to 18.90% peanut oil, 3.08% to 4.22% milk powder, 0.01% to 0.10% ethyl butyrate, 1.34% to 1.70% potassium sorbate and 0.01% to 0.50% insecticide by mass fraction.

[0006] Preferably, the meat floss is one or more of pork floss, chicken floss, and fish floss.

[0007] Preferably, the insecticide is one or more of dinotefuran, indoxacarb, fipronil, avermectin, spinosad, hydrazone, and pyriproxyfen.

[0008] Preferably, the red fire ant bait is composed, by mass fraction, of 61.99% corn flour, 16.57% pork floss, 16.89% peanut oil, 3.08% milk powder, 0.05% ethyl butyrate, 1.39% potassium sorbate and 0.05% dinotefuran.

[0009] The dinotefuran is also known as 1-methyl-2-nitro-3-(tetrahydro-3-furanmethyl)guanidine; (EZ)-(RS)-1-methyl-2-nitro-3-(tetrahydro-3-furanmethyl)guanidine; N-methyl-N'-nitro-N"-[(tetrahydro-3-furan)methyl]guanidine, indoxacarb is also known as (2,4-di-tert-butylphenyl) phosphite triester; 7-chloro-2,5-dihydro-2-[N-(methoxyformyl)-4-(trifluoromethoxy)aniline formyl] indole Methyl [1,2-E][1,3,4]triazine-4A(3H)-carboxylate and fipronil are also known as (RS)-5-amino-1-(2,6-dichloro-4-trifluoromethylphenyl)-4-trifluoromethylsulfinylpyrazole-3-carbonitrile, hydrazone is also known as 5,5-dimethylperhydropyrimidin-2-one 4-trifluoromethyl-α-(4-trifluoromethylphenyl)cinnamidine hydrazone, and pyriproxyfen is also known as 4-phenoxyphenyl (RS)-2-(2-pyridyloxy)propyl ether.

[0010] The second purpose of the present invention is to protect the preparation method of the red fire ant bait, which is obtained by mixing and stirring various raw materials, granulating and drying.

[0011] Preferably, the above preparation method specifically comprises the following steps:

[0012] a. Grinding: Grind corn into an average particle size of 0.3-1.5 mm, and grind meat floss into an average particle size of 1.5-2.5 mm;

[0013] b. Stirring: Put the crushed corn flour, milk powder, potassium sorbate and dinotefuran into a blender, stir thoroughly, then add a mixture of peanut oil and ethyl butyrate, meat floss, and stir thoroughly to obtain bait;

[0014] c. Granulation: Put the mixed bait into a granulator and use a mesh sieve with a pore size of 1 to 2 mm for granulation.

[0015] More preferably, the average particle size of the bait particles in step (c) is 1.5 mm.

[0016] Compared with the prior art, the invention has the following advantages: the red fire ant bait is based on the base bait (corn flour, meat floss, peanut oil, milk powder), potassium sorbate keeps the freshness of the bait, a trace amount of ethyl butyrate is added to further stimulate the appetite of the red fire ants, and a granulator is used to form particles with an average particle size of 1.5 mm, which are suitable for the red fire ants to carry, so that the bait is further attractive to the red fire ants. Specific implementation method:

[0017] The following examples are provided to further illustrate the present invention, rather than to limit the present invention.

[0018] Unless otherwise defined, all professional terms used hereinafter have the same meaning as those generally understood by those skilled in the art. The professional terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present invention. Unless otherwise specified, the experimental materials and reagents herein are conventional commercial products in the art.

[0019] 1. Test basis

[0020] Investigate the stability of bait particles.

[0021] After feeding the red fire ants in the morning, noon, afternoon and saturated with ordinary biscuit powder, the palatability of the formula was judged by the length of time it took to move the test bait particles.

[0022] The efficacy test was conducted according to the scheme set out in GB / T 13917.7-2009 “Indoor efficacy test and evaluation of public health insecticides for pesticide registration Part 7: Bait”.

[0023] 2. Test methods

[0024] 2.1 Storage stability

[0025] Store in ziplock bag at room temperature for 2 months

[0026] 2.2 Palatability test subjects

[0027] Ants: Red fire ants (Solenopsis invicta Buren), 20 nests are raised in a plastic bucket (5L), and a circle of vaseline is applied on the inner edge of the bucket mouth.

[0028] 2.3 Subjects of efficacy test

[0029] Ants: Red imported fire ants (Solenopsis invicta Buren) were worker ants over 3 days old and were raised in the laboratory.

[0030] Use an enamel square dish device (white, 400mm×300mm×40mm). Apply a circle of vaseline on the inner edge of the enamel square dish, put 100 ants in, and after the test insects resume normal activities, place equal amounts of bait and ant feed at the diagonal of the enamel square dish and about 50mm away from the edge. Observe the death of ants day by day, and take out and count the dead ants. Observe continuously until the 7th day after feeding. The test should be repeated three times or more, and set a blank control with normal feed. The test device should be cleaned at the end of each test.

[0031] 3 Results and evaluation

[0032] 3.1 Stability evaluation criteria

[0033] If the particles can still maintain their original state when stored at room temperature for more than 2 months, and have good fluidity, it is of excellent quality. If there is agglomeration, it is of medium quality. If the particles are not formed, it is of inferior quality.

[0034] 3.2 Palatability evaluation criteria

[0035] The palatability is better than ordinary biscuit powder particles, which is excellent, equivalent to it, which is medium, and worse than it, which is inferior.

[0036] 3.3 Efficacy test results and evaluation

[0037] 3.3.1 Calculation method of drug efficacy

[0038] Calculate the mortality rate and corrected mortality rate according to formula (1) and (2), and keep the result to two decimal places. If the mortality rate of the blank control group is less than 5%, no correction is required; if the mortality rate of the blank control group is 5%-20%, correction should be made; if the mortality rate of the blank control group is greater than 20%, the experiment is invalid. Mortality rate, %; number of insects, in pieces.

[0039]

[0040]

[0041] 3.3.2 Efficacy evaluation criteria

[0042] According to GB / T 13917.7-2009 "Indoor efficacy test and evaluation of public health insecticides for pesticide registration Part 7: Bait", the efficacy results are divided into two levels, A and B. Products that do not meet the B level standard are considered unqualified products.

[0043] Ants: Mortality on the 7th day after feeding, Grade A: 100%, Grade B: ≥90%.

[0044] Example 1

[0045] a. Determination of formula

[0046] Formula 1: pork floss 10.721g, corn flour 40.105g, peanut oil 10.927g, milk powder 1.990g, potassium sorbate 0.898g, ethyl butyrate 0.030g, dinotefuran 0.030g;

[0047] Formula 2: pork floss 10.001g, corn flour 30.115g, peanut oil 10.029g, milk powder 2.002g, potassium sorbate 0.900g, ethyl butyrate 0.005g, dinotefuran 0.005g;

[0048] Formula 3: pork floss 7.965g, corn flour 29.029g, peanut oil 7.894g, milk powder 2.021g, potassium sorbate 0.643g, ethyl butyrate 0.050g, dinotefuran 0.240g;

[0049] Compared with formula 1, the following formulas (4-6) are used as controls:

[0050] Formula 4: pork floss 10.721g, corn flour 40.105g, peanut oil 10.927g, milk powder 1.990g, potassium sorbate 0.898g, octyl acetate 0.030g, dinotefuran 0.030g;

[0051] Formula 5: pork floss 10.721g, corn flour 40.105g, peanut oil 10.927g, milk powder 1.990g, potassium sorbate 0.898g, ethyl acetate 0.030g, dinotefuran 0.030g;

[0052] Recipe 6: pork floss 10.721g, corn flour 40.135g, peanut oil 10.927g, milk powder 1.990g, potassium sorbate 0.898g, and dinotefuran 0.030g.

[0053] Weigh the pork floss, corn flour, peanut oil, milk powder, potassium sorbate, ethyl butyrate, octyl acetate, ethyl acetate and dinotefuran in the above formula respectively.

[0054] (a) Grinding: Grind corn (average particle size 0.3-1.5 mm) and meat floss (average particle size 1.5-2.5 mm) respectively;

[0055] (b) Stirring: Put the crushed corn flour, milk powder, potassium sorbate and dinotefuran into a blender, stir thoroughly for 0.5 h, then add the peanut oil mixture (peanut oil + ethyl butyrate) and meat floss, and stir thoroughly for another 1 h;

[0056] (c) Granulation: Put the mixed bait into a granulator and use a mesh sieve with a pore size of 1.5 mm to granulate (average particle size 1-2 mm), and pack it in ziplock bags for later use.

[0057] b. Investigation on the stability of formula particles

[0058] The bait granules were stored in a ziplock bag at room temperature for 2 months. The observation results are shown in Table 1.

[0059] Table 1 Stability of granules with different formulations

[0060]

[0061] Example 2

[0062] Same as Example 1, except that:

[0063] Palatability of baits at different time periods

[0064] On June 22, at 10 a.m., bait granules (2 g each) were placed, and the formula (1-3) was moved in 5 minutes; at 13 p.m., bait granules were placed, and the formula (1-3) was moved in 5 minutes; at 17 p.m., bait granules were placed, and the formula (1-3) was moved in 10 minutes. It was not affected by time, and I really liked to eat it. The bait granules with ethyl butyrate added (formula (1-3)) were more attractive than ham sausage and biscuit powder, and more attractive than the control (without ethyl butyrate) bait granules (formula (4-6)), indicating that ethyl butyrate has a significant attractant effect on red fire ants; the bait granules with the same dose of octyl acetate and ethyl acetate (formula (4-5)) were not significantly different from the control (formula 6), indicating that octyl acetate and ethyl acetate have no attractant effect on red fire ants. See Table 2.

[0065] Table 2 Palatability of baits to red fire ants at different times

[0066]

[0067] Example 3

[0068] Same as Example 1, except that:

[0069] Palatability after saturation feeding of ordinary biscuit powder

[0070] At 10:00 am on June 26, the ants were saturated with ordinary biscuit powder particles (10g). The ants came out to move the particles, but 10 minutes later, most of the ants were crawling on the biscuit powder, and only a small part (25%) of the ants were moving the particles. It seems that they don't like ordinary biscuit powder. At 17:40 in the afternoon of the same day, it was found that there was no activity on the soil surface, and the biscuit powder fed in the morning was still there. At this time, the test bait particles (formula 1-3) (2g) were placed, and the ants came out to move the particles, but it took 20 minutes to move the particles, which was longer than before. The factor of hunger was ruled out.

[0071] At 10:00 am on June 30, the ordinary biscuit powder particles placed 4 days ago were still found, indicating that red fire ants do not like ordinary biscuit powder particles. After the test bait particles (formula 1-3) (2g) were placed, the red fire ants still came out to move. But it took 15 minutes to move all the way. This once again confirmed that this formula bait is very palatable to red fire ants. See Table 3.

[0072] Table 3 Palatability after saturated feeding of ordinary biscuit powder

[0073]

[0074] Example 4

[0075] Same as Example 1, except that:

[0076] The efficacy of baits against red fire ants

[0077] The mortality rate of the baits (formulas 1-3) in this test was 100% on the first day after feeding, which is classified as Class A. The mortality rate of the control formulas (4-6) reached 100% on the third day after feeding, and the difference in quick effect was significant. This shows that the baits with ethyl butyrate added have a strong attraction to red fire ants, promote the appetite of red fire ants, and accelerate the stomach poisoning effect of the baits on red fire ants. See Table 4.

[0078] Table 4 Statistical table of the efficacy of baits against red fire ants

[0079]

[0080]

[0081] The description of the above embodiments is only used to help understand the technical solution and core ideas of the present invention. It should be pointed out that for technicians in this technical field, several improvements and modifications can be made to the present invention without departing from the principles of the present invention. These improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A red fire ant bait, characterized in that: The composition thereof is calculated by mass fraction, comprising 56.76% to 61.99% corn flour, 16.57% to 18.85% meat floss, 16.50% to 18.90% peanut oil, 3.08% to 4.22% milk powder, 0.01% to 0.10% ethyl butyrate, 1.34% to 1.70% potassium sorbate and 0.01% to 0.50% pesticide.

2. The red fire ant bait according to claim 1, characterized in that The meat floss is one or more of pork floss, chicken floss and fish floss.

3. The red fire ant bait according to claim 1, characterized in that The insecticide is one or more of dinotefuran, indoxacarb, fipronil, avermectin, spinosad, hydrazone and pyriproxyfen.

4. The red fire ant bait according to claim 1, characterized in that The red fire ant bait consists of 61.99% corn flour, 16.57% pork floss, 16.89% peanut oil, 3.08% milk powder, 0.05% ethyl butyrate, 1.39% potassium sorbate and 0.05% dinotefuran, by mass fraction.

5. A method for preparing the red fire ant bait according to claim 1, characterized in that: The process comprises mixing and stirring various raw materials, granulating and drying.

6. The preparation method according to claim 5, characterized in that: The specific steps include: a. Grinding: Grind corn into an average particle size of 0.3-1.5 mm, and grind meat floss into an average particle size of 1.5-2.5 mm; b. Stirring: Put the crushed corn flour, milk powder, potassium sorbate and dinotefuran into a blender, stir thoroughly, then add a mixture of peanut oil and ethyl butyrate, meat floss, and stir thoroughly to obtain bait; c. Granulation: Put the mixed bait into a granulator and use a mesh sieve with a pore size of 1 to 2 mm for granulation.

7. The preparation method according to claim 6, characterized in that: The average particle size of the bait particles described in step c is 1.5 mm.

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