Plant sunscreen lotion with anti-ultraviolet effect and application of plant sunscreen lotion in sunscreen product

Abstract

The invention discloses a plant sunscreen lotion with an anti-ultraviolet effect and application of the plant sunscreen lotion in a sunscreen product, and particularly belongs to the field of preparations for dressing and makeup. The plant sunscreen lotion with the anti-ultraviolet effect is prepared from a plant composition with the anti-ultraviolet effect, auxiliary materials and a solvent, the plant composition with the anti-ultraviolet effect comprises a polygonatum cyrtonema rhizome / root extract, a honeysuckle flower extract, a sophora flower bud extract, a centella asiatica extract and a butterflybush flower extract. The plant composition provided by the invention has the advantages that the components supplement each other and have a synergistic effect, and the plant composition has a good anti-oxidation effect and can effectively resist ultraviolet damage and play a photoprotection role; the sunscreen lotion containing the plant composition with the anti-ultraviolet effect, provided by the invention, has an excellent sunscreen effect.

Description

Technical Field

[0001] The invention belongs to the field of cosmetic preparations, and in particular relates to a plant sunscreen lotion with anti-ultraviolet efficacy and application of the plant sunscreen lotion in sunscreen products. Background Art

[0002] With global climate change and the increase in people's outdoor activities, the damage caused by ultraviolet radiation to the skin has received increasing attention. Traditional sunscreen products mainly rely on chemical sunscreens or physical sunscreens to block or absorb ultraviolet rays, but these ingredients may cause skin irritation, allergies or other adverse reactions. Therefore, the development of new sunscreen products that are safe, effective and skin-friendly has become a hot topic in current research.

[0003] In recent years, plant extracts have been widely used in the cosmetics field due to their natural origin, low irritation and multi-effects. Many plants contain active ingredients with anti-ultraviolet effects, such as polyphenols, flavonoids, carotenoids, etc. These ingredients can absorb, scatter or convert ultraviolet rays, thereby reducing the damage of ultraviolet rays to the skin. In addition, plant extracts also have multiple skin care effects such as anti-oxidation, anti-inflammatory, moisturizing, etc., which can improve skin condition and enhance skin's defense ability.

[0004] However, the anti-ultraviolet effect of a single plant extract is often limited, and the interaction between different plant extracts may also affect the sunscreen effect. Therefore, how to screen and optimize plant compositions to achieve synergistic effects and improve anti-ultraviolet effects has become a difficulty and focus of current research.

[0005] At present, there are some sunscreen products containing plant extracts on the market, but these products often have problems such as unstable sunscreen effect, complex formula, high cost, etc. Therefore, it is of great significance to develop a plant composition with stable anti-ultraviolet effect, simple formula, moderate cost and its application in sunscreen products. Summary of the invention

[0006] In view of the deficiencies in the prior art, the object of the present invention is to provide a plant composition with anti-ultraviolet efficacy, a sunscreen lotion and the use of the same in sunscreen products. The plant composition achieves synergistic enhancement and improves the anti-ultraviolet effect through the combination and scientific proportion of multiple plant extracts with anti-ultraviolet efficacy.

[0007] In order to achieve the above object, the present invention discloses the following technical solutions:

[0008] In a first aspect, the present invention provides a plant composition with anti-ultraviolet efficacy, comprising rhizome / root extract of Polygonatum cyrtonema, honeysuckle flower extract, Sophora japonica bud extract, Centella asiatica extract and Buddleja bud extract.

[0009] Preferably, the following components are included by weight:

[0010]

[0011] In a second aspect, the present invention provides a plant sunscreen lotion with anti-ultraviolet efficacy, comprising the plant composition with anti-ultraviolet efficacy described in the first aspect.

[0012] Preferably, the amount of the plant composition with anti-ultraviolet efficacy added to the plant sunscreen lotion is 0.015-0.02 wt %.

[0013] More preferably, it also includes auxiliary materials and solvents,

[0014] The auxiliary materials include film formers, emulsion stabilizers, thickeners, moisturizers, preservatives, antioxidants and sunscreens, and the solvent is deionized water.

[0015] More preferably, the film former is at least one of triethoxycaprylylsilane, octyl polymethicone, polydimethylsiloxane, PEG-10 polydimethylsiloxane and trimethylsiloxysilicate;

[0016] The emulsifier stabilizer is at least one of potassium cetyl phosphate, arachidyl alcohol, behenyl alcohol, arachidyl glucoside, cetearyl alcohol, coconut glucoside, diisopropyl sebacate and polyhydroxystearic acid;

[0017] The thickener is at least one of ammonium acryloyldimethyltaurate / VP copolymer, vinyl polydimethylsiloxane / polymethylsiloxane silsesquioxane cross-linked polymer, silica and disteardimethylammonium hectorite;

[0018] The moisturizer is at least one of sunflower seed oil, glyceryl caprylate, 1,2-hexanediol, panthenol, butylene glycol and 1,2-pentanediol;

[0019] The sunscreen is at least one of isopentyl p-methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, methylene bis-benzotriazolyl tetramethylbutylphenol, butyl octanol salicylate and titanium dioxide;

[0020] The antioxidant is vitamin E;

[0021] The preservative is at least one of p-hydroxyacetophenone and 1,2-hexanediol.

[0022] In a third aspect, the present invention provides a method for preparing the plant sunscreen lotion according to the second aspect, comprising the following steps:

[0023] The film former, the emulsion stabilizer, the thickener, the moisturizer, the preservative, the antioxidant, the sunscreen, the plant composition and deionized water are uniformly mixed at 40-80° C. to obtain the plant sunscreen lotion.

[0024] In a fourth aspect, the present invention provides the use of the plant composition with anti-ultraviolet efficacy described in the first aspect in the preparation of sunscreen lotion, sunscreen cream, sunscreen liquid, sunscreen spray, sunscreen gel, isolation cream, isolation milk or BB cream.

[0025] In the present invention:

[0026] The rhizome / root extract of Polygonatum sibiricum is rich in active ingredients such as polysaccharides, steroidal saponins, triterpenes, alkaloids, lignans, flavonoids, phytosterols and volatile oils. It has a certain ability to absorb ultraviolet rays, can scavenge the active oxygen free radicals induced by ultraviolet radiation, and has a certain protective effect against ultraviolet rays.

[0027] Honeysuckle extract contains active ingredients such as luteolin, luteolin, chlorogenic acid and its isomers, triterpenoid saponins, etc. The flavonoids in honeysuckle extract can absorb ultraviolet energy, thereby reducing the direct damage of ultraviolet rays to the skin. At the same time, chlorogenic acid, flavonoids and other ingredients in honeysuckle extract have strong antioxidant capacity, which can effectively remove free radicals produced after the skin is exposed to ultraviolet light, reduce the damage to the skin caused by oxidative stress, and indirectly protect the skin from ultraviolet damage.

[0028] Sophora japonica bud extract is rich in active ingredients such as flavonoids, isoflavones, saponins, etc., which have a certain anti-UV ability. At the same time, Sophora japonica bud extract can reduce the oxidative stress damage and inflammatory response caused by ultraviolet rays, and indirectly play an anti-UV damage effect.

[0029] Centella asiatica extract contains rich active ingredients, such as triterpenoid saponins, polysaccharides and flavonoids, which can effectively promote wound healing and repair damaged skin. It has significant anti-inflammatory effects and can effectively reduce skin redness and pain. At the same time, it can effectively scavenge free radicals, protect the skin from environmental damage, and delay aging.

[0030] The flavonoids contained in the Buddleja flower extract have a strong broad-spectrum absorption capacity for ultraviolet rays, can directly absorb ultraviolet radiation, and then play an anti-ultraviolet radiation effect. At the same time, the Buddleja flower extract can eliminate the active oxygen free radicals induced by ultraviolet radiation, and reduce the oxidative damage and inflammatory response caused by ultraviolet rays.

[0031] Beneficial effects of the present invention:

[0032] 1. In the composition provided by the present invention, the rhizome / root extract of Polygonatum cyrtonema, honeysuckle flower extract, Sophora japonica bud extract, Centella asiatica extract and Buddleja bud extract complement each other and synergize with each other, and have a good antioxidant effect;

[0033] 2. The present invention's composition of Polygonatum cyrtonema rhizome / root extract, honeysuckle flower extract, Sophora japonica bud extract, Centella asiatica extract and Buddleja bud extract can effectively resist ultraviolet damage and play a role in light protection;

[0034] 3. The sunscreen lotion provided by the present invention containing the composition of the rhizome / root extract of Polygonatum cyrtonema, honeysuckle flower extract, sophora japonica bud extract, Centella asiatica extract and Buddleja bud extract has excellent sunscreen effect. DETAILED DESCRIPTION

[0035] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. It should be understood by those skilled in the art that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0036] Unless otherwise specified, the test methods used in the specific implementation methods are all conventional methods; the materials, reagents, etc. used, unless otherwise specified, can be obtained from commercial channels; the percentages mentioned in the specific implementation methods, unless otherwise specified, are all mass percentages.

[0037] In the present invention:

[0038] The rhizome / root extract of Polygonatum multiflorum is the rhizome / root extract of Polygonatum multiflorum (POLYGONATUM MULTIFLORUM), purchased from Jiangsu Duoyang Bioengineering Technology Co., Ltd., specification 10:1, water-soluble powder;

[0039] Honeysuckle flower extract is Lonicera japonica flower extract, purchased from Shaanxi New Horizon Biotechnology Co., Ltd., specification 10:1, water-soluble powder;

[0040] Sophora japonica bud extract is Sophora japonica bud extract, purchased from Huzhou Purui Biopharmaceutical Technology Co., Ltd., specification 10:1, process: water extraction;

[0041] Centella asiatica extract is Centella asiatica extract, purchased from Sinopharm (Guangzhou) International Pharmaceutical and Health Co., Ltd., specification: purity 30%, process: water extraction;

[0042] Buddleja officinalis extract was purchased from Xi'an Senran Bioengineering Co., Ltd., specification 10:1, water-soluble powder;

[0043] Other raw materials are commercially available.

[0044] Preparation of plant composition:

[0045] The raw materials were accurately weighed according to the mass fractions of the raw materials in Table 1, and then placed in a high-speed mixer for uniform mixing to obtain a plant composition.

[0046] Table 1. Raw materials of plant composition by weight

[0047]

[0048]

[0049] Note: “-” in the table means no addition.

[0050] Performance test of plant composition:

[0051] The above compositions 1-8 were mixed with deionized water to prepare test samples with a concentration of 0.02 wt %.

[0052] 1 Antioxidant Test:

[0053] This test tests the DPPH free radical scavenging ability of the test samples of compositions 1 to 8. The DPPH free radical scavenging ability reflects the antioxidant ability of a substance to a certain extent.

[0054] The test method is as follows:

[0055] Accurately weigh the DPPH reagent and prepare a 0.04 mg / mL DPPH solution with 50 v / v% ethanol aqueous solution. Under light-proof conditions, add the DPPH solution and different test substances to a 96-well plate, mix thoroughly, incubate at room temperature in the dark for 30 minutes, and measure the absorbance at 517 nm on an ELISA reader. Each measurement requires three groups of samples, blanks, and controls, with three parallels in each group, as follows:

[0056] Sample group: 100μL sample solution + 100μL DPPH solution

[0057] Blank group: 100 μL sample solution + 100 μL 50 v / v% ethanol aqueous solution

[0058] Control group: 100 μL deionized water + 100 μL DPPH solution

[0059] The DPPH clearance rate was calculated according to the following formula:

[0060] DPPH clearance rate = [1-(sample group-blank group) / control group] × 100%

[0061] The test results are shown in Table 2:

[0062] Table 2 Antioxidant test results

[0063] Group DPPH removal rate / % Composition 1 89.36 Composition 2 90.11 Composition 3 88.29 Composition 4 44.78 Composition 5 48.42 Composition 6 42.36 Composition 7 31.08 Composition 8 29.28

[0064] Result analysis:

[0065] According to the results in Table 2, it can be seen that compositions 1-3 have excellent DPPH free radical scavenging ability, and compared with compositions 4-8, it is found that the lack of any one or two components will reduce the free radical scavenging rate of the composition, which indicates that the rhizome / root extract of Polygonatum cyrtonema, honeysuckle extract, Sophora japonica bud extract, Centella asiatica extract and Buddleja monnieri extract in the composition complement each other and synergize with each other, and have good antioxidant effects.

[0066] 2 Light protection test:

[0067] This test is to test the cell survival rate of compositions 1 to 8. The survival rate of human immortalized keratinocytes (HaCat) after ultraviolet irradiation can reflect the protective effect of a substance against ultraviolet rays to a certain extent.

[0068] The test method is as follows:

[0069] First, the cell-safe concentration of the composition was tested by MTT cytotoxicity assay.

[0070] According to 2×10 5 The immortalized keratinocyte suspension was inoculated into a 96-well plate at a density of cells / well, 100 μL / well, and cultured in a 37° C., 5% CO 2 incubator for 24 h.

[0071] Using complete culture medium (DMEM high-glucose complete culture medium, product number MA10001A, purchased from Beijing Anrui Special Biotechnology Co., Ltd.) as the diluent, the composition 1-8 test samples were diluted to the cell safety test concentration and then added to the cells respectively, 100 μL / well, wherein the positive control group and the negative control group replaced the composition with deionized water of the same mass and cultured in a CO2 incubator at 37°C and a volume fraction of 5% for 24 hours.

[0072] The cells in the composition 1-8 groups and the positive control group were irradiated with UV for 20 minutes respectively, and the negative control group was not irradiated with light. They were then placed in an incubator and incubated for 24 hours. Finally, the MTT test was used to detect the changes in cell viability.

[0073] The calculation formula is as follows:

[0074] Cell viability percentage (%) = average OD value of sample wells / average OD value of negative control group × 100%

[0075] The test results are shown in Table 3:

[0076] Table 3 Light protection test results

[0077]

[0078] Result analysis:

[0079] According to the results in Table 3, compared with the positive control group, the HaCat cell activity of the cells treated with compositions 1-3 was significantly increased after ultraviolet irradiation, and was higher than that of compositions 4-8, which indicates that the composition of the present invention can effectively resist ultraviolet damage and play a role in light protection.

[0080] Preparation of sunscreen lotion:

[0081] Step S1-1. According to the mass percentage of the raw materials in Table 4, the raw materials are accurately weighed, and each raw material in component A is placed in an emulsification reaction pot, and dissolved and mixed uniformly at 80°C and a speed of 200r / min to obtain a mixture A;

[0082] Step S1-2. Take the deionized water of component B in Table 4 and place it in a reaction pot and heat it to 80°C, add glyceryl caprylate, 1,2-hexanediol, panthenol, butylene glycol, 1,2-pentanediol, vinyl polydimethylsiloxane / polymethylsiloxane silsesquioxane crosslinked polymer in sequence, stir and mix evenly at 200r / min, increase the speed to 500r / min after mixing evenly, add silica and disteardimethylammonium hectorite, mix evenly, and obtain a mixture B;

[0083] Step S1-3. The mixture A is pumped into the mixture B at 80°C, and the mixture is quickly homogenized and dispersed at a speed of 5000 r / min. After homogenization and dispersion for 10 minutes, the mixture is mechanically stirred at 200 r / min for 30 minutes to obtain a mixture AB;

[0084] Step S1-4. Dissolve the raw materials of component D and mix them evenly to obtain a mixture D. Cool the mixture AB to 55°C, add the raw materials of component C and mixture D in sequence, mix and stir at 500 r / min to obtain a mixture ABCD.

[0085] Step S1-5. After the mixture ABCD is cooled to 40° C., the plant composition 2 and the remaining deionized water are added thereto, and mixed at a speed of 200 r / min to obtain a sunscreen lotion.

[0086] Table 4 Application Example Raw Materials Mass Percentage

[0087]

[0088]

[0089] SPF value test for topical products

[0090] The test method is as follows:

[0091] 1. Materials and Methods

[0092] 1. Object to be measured: Application examples 1-3.

[0093] 2. Reference product: SPF value ≈ 15.4, prepared according to the standard formula of medium and high SPF standard product (P2) in the "Technical Specification for Safety of Cosmetics" (2015 edition).

[0094] 3. Subjects: 3 persons in total, 3 females, aged 34 to 36 years old, who met the voluntary inclusion criteria.

[0095] 4. Light source: Daylight simulator xenon arc lamp, all performance indicators meet the measurement specification requirements.

[0096] 5. Inspection method:

[0097] The test is carried out in accordance with the specific requirements of the currently valid technical specifications. The subject is in a prone position and the back is irradiated. The minimum erythema dose (MED value) of the subject's skin to ultraviolet radiation is predicted 24 hours before the test, and the ultraviolet radiation dose is adjusted according to the predicted results for testing the object being tested.

[0098] On the day of the test, a spot no smaller than 30 cm is selected on the back of the subject. 2 For normal skin area, 2.00±0.05mg / cm 2 The test object or reference substance is evenly applied to the above-mentioned area with the dosage of 100 mg / kg. Then the irradiation dose is selected according to the specification requirements, and irradiation is performed in three conditions: ① The test object is not applied to the subject's skin; ② The reference substance is applied; ③ The test object is applied. Observe the experimental results after 24 hours and record the MED values ​​in the three conditions respectively.

[0099] 6. SPF value calculation method:

[0100] The SPF value of the test article or reference article for protecting a single subject is expressed by the following formula:

[0101] SPF = MED value of protected skin / MED value of unprotected skin.

[0102] The individual SPF value is required to be accurate to one decimal place. Calculate the arithmetic mean of the SPF values ​​of all subjects protected by the tested object, and take the integer part as the SPF value of the measured sample.

[0103] 7. The test results are shown in Table 5:

[0104] Table 5 SPF value test results

[0105]

[0106] PFA value test of external agent

[0107] The test method is as follows:

[0108] 1. Object to be measured: Application examples 1-3.

[0109] 2. Reference substance: PFA value ≈ 4.1, prepared according to the standard formula of the standard substance in the "Technical Specification for Safety of Cosmetics" (2015 edition).

[0110] 3. Subjects: 3 persons in total, 3 females, aged 30 to 36 years old, who met the voluntary inclusion criteria.

[0111] 4. Light source: Daylight simulator xenon arc lamp, all performance indicators meet the measurement specification requirements.

[0112] 5. Test method: Test in accordance with the specific requirements of the currently valid technical specifications. The subject lies prone and the back is exposed to the radiation. The minimum blackening amount (MPPD value) of the subject's skin to ultraviolet radiation is predicted 24 hours before the test, and the ultraviolet radiation amount is adjusted according to the predicted results for testing the object. The test first selects a spot no less than 30cm on the subject's back. 2 For normal skin area, (2.00±0.05)mg / cm 2 Apply the test object or reference product evenly on the above area with the dosage; then select the UVA exposure amount according to the specification requirements, and irradiate in three situations: ① No test object is applied to the subject's skin; ② The reference product is applied; ③ The test object is applied. Observe the experimental results after 2 to 4 hours, and record the MPPD values ​​in the three situations respectively.

[0113] 6. Calculation method of PFA value:

[0114] The PFA value of the test substance or reference substance for a single subject is expressed by the following formula:

[0115] PFA = MPPD value of protected skin / MPPD value of unprotected skin.

[0116] The individual PFA value is required to be accurate to one decimal place. The arithmetic mean of the PFA values ​​of all subjects protected by the measured object is calculated, and the integer part is the PFA value of the measured sample.

[0117] 7. The test results are shown in Table 6:

[0118] Table 6 PFA value test results

[0119]

[0120] Result analysis:

[0121] SPF stands for Sun Protection Factor. The larger the SPF value, the better the effect of preventing sunburn / sunburn.

[0122] PFA is called UVA protection index. The larger the PFA value, the better the UVA protection effect.

[0123] According to the results of Table 5 and Table 6, the sunscreen lotion provided by the present invention has excellent sunscreen effect, and the composition of Polygonatum cyrtonema rhizome / root extract, Honeysuckle flower extract, Sophora japonica bud extract, Centella asiatica extract and Buddleja bud extract can be used in sunscreen products to provide good light protection for skin topical agents. At the same time, the higher the concentration of the plant composition, the better the sunscreen performance of the sunscreen lotion, showing a certain concentration dependence.

[0124] Although the specific embodiments of the present invention are described above, it should be understood by those skilled in the art that this is only for illustration and the protection scope of the present invention is defined by the appended claims. Those skilled in the art may make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, but these changes and modifications all fall within the protection scope of the present invention.

Claims

1. A plant composition with anti-ultraviolet effect, characterized in that: Contains Polygonatum multiflorum rhizome / root extract, Honeysuckle flower extract, Sophora japonica bud extract, Centella asiatica extract, and Buddleja chinensis flower extract.

2. The plant composition with anti-ultraviolet efficacy according to claim 1, characterized in that: By mass, it contains the following components:

3. A plant sunscreen lotion with anti-ultraviolet effect, characterized in that: A plant composition with anti-ultraviolet efficacy comprising the plant composition according to claim 1 or 2.

4. The plant sunscreen lotion according to claim 3, characterized in that: The amount of the plant composition with anti-ultraviolet efficacy added to the plant sunscreen lotion is 0.015-0.02 wt %.

5. The plant sunscreen lotion according to claim 3, characterized in that: The invention also comprises auxiliary materials and solvents. The auxiliary materials comprise a film former, an emulsion stabilizer, a thickener, a moisturizer, a preservative, an antioxidant and a sunscreen. The solvent is deionized water.

6. The plant sunscreen lotion according to claim 5, characterized in that: The film former is at least one of triethoxycaprylylsilane, octyl polymethicone, polydimethylsiloxane, PEG-10 polydimethylsiloxane and trimethylsiloxysilicate; The emulsifier stabilizer is at least one of potassium cetyl phosphate, arachidyl alcohol, behenyl alcohol, arachidyl glucoside, cetearyl alcohol, coconut glucoside, diisopropyl sebacate and polyhydroxystearic acid; The thickener is at least one of ammonium acryloyldimethyltaurate / VP copolymer, vinyl polydimethylsiloxane / polymethylsiloxane silsesquioxane cross-linked polymer, silica and disteardimethylammonium hectorite; The moisturizer is at least one of sunflower seed oil, glyceryl caprylate, 1,2-hexanediol, panthenol, butylene glycol and 1,2-pentanediol; The sunscreen is at least one of isopentyl p-methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, methylene bis-benzotriazolyl tetramethylbutylphenol, butyl octanol salicylate and titanium dioxide.

7. The method for preparing the plant sunscreen lotion according to claim 5 or 6, characterized in that: The following steps are involved: The plant sunscreen lotion is obtained by uniformly mixing a film former, an emulsifier stabilizer, a thickener, a moisturizer, a preservative, an antioxidant, a sunscreen agent, the plant composition and deionized water at 40-80° C.

8. Use of the plant composition with anti-ultraviolet efficacy according to claim 1 or 2 in the preparation of sunscreen lotion, sunscreen cream, sunscreen liquid, sunscreen spray, sunscreen gel, isolation cream, isolation milk or BB cream.

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