Centella asiatica fermentation product as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and discloses application of eurotium cristatum fermented plant components to preparation of cosmetics. The plant component is centella asiatica; the classification name of the eurotium cristatum is eurotium cristatum, and the preservation number of the eurotium cristatum is CGMCC (China General Microbiological Culture Collection Center) NO: 40145. The preservation date is March 10, 2022, and the preservation unit is China General Microbiological Culture Collection Center (CGMCC). According to the invention, the eurotium cristatum is adopted to ferment the rehydrated centella asiatica, so that the DPPH removing capability of the centella asiatica extract can be improved. Meanwhile, the invention further provides a preparation method and application of the centella asiatica fermentation product.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and specifically to a Centella asiatica fermentation product, a preparation method thereof, and an application thereof. Background Art

[0002] The applicant has filed a patent application with the application number 202410518179.3 and the theme of a Eurotium cristatum, uses thereof;

[0003] In preliminary studies, we have demonstrated that the tea fermentation product of this strain acts on the fatty liver model of mice. During the continuous feeding process, it is found that the tea fermentation product of this strain can significantly reduce the contents of triglyceride and low-density cholesterol.

[0004] In new studies, fermentation studies on Centella asiatica have been carried out and preliminary results have been obtained. Summary of the Invention

[0005] The purpose of the present invention is to provide a Centella asiatica fermentation product. By using Eurotium cristatum to ferment the rehydrated Centella asiatica, the DPPH scavenging ability of the Centella asiatica extract can be improved.

[0006] Meanwhile, the present invention also provides a preparation method and an application of the Centella asiatica fermentation product.

[0007] To achieve the above purpose, the present invention provides the following technical solution: the use of Eurotium cristatum to ferment plant components to prepare cosmetics;

[0008] The plant component is Centella asiatica;

[0009] The taxonomic name of the Eurotium cristatum is Eurotium cristatum, and the preservation number is: CGMCC NO: 40145; the preservation date is March 10, 2022, and the preservation unit is the General Microbiological Center of the China Committee for Culture Collection of Microorganisms.

[0010] Meanwhile, the present invention also discloses a preparation method of a Centella asiatica fermentation product, which includes the following steps:

[0011] Step 1: Rehydrate Centella asiatica to a moisture content of about 30%;

[0012] Step 2: Sterilize, sterilize the rehydrated Centella asiatica;

[0013] Step 3: Inoculate the Eurotium cristatum as described in claim 1 on the rehydrated Centella asiatica, and the inoculation amount per kilogram of Centella asiatica before rehydration is 0.1 - 0.5 g of bacterial powder; perform aerobic fermentation at room temperature for 15 days;

[0014] Step 4: Collect the fermentation product of Step 3 and dry it.

[0015] In the above preparation method, the dried fermentation product obtained in step 4 is pulverized, and the pulverized powder is subjected to alcohol extraction with a 95 vol% ethanol solution, and then filtered to obtain a filtrate, and the filtrate is concentrated under reduced pressure to obtain a dry powder.

[0016] In the above preparation method, the temperature of the alcohol extraction is 40 to 50 °C, and the alcohol extraction time is 3 to 5 hours.

[0017] Finally, the present invention also discloses a Centella asiatica fermentation product prepared by any of the above methods.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0019] The alcohol-soluble cell lysate of Eurotium cristatum of the present invention has excellent free radical scavenging ability. After fermenting Centella asiatica with Eurotium cristatum, compared with the alcohol extract of Centella asiatica, its free radical scavenging ability has been further significantly improved. Detailed Embodiments

[0020] Next, the technical solutions of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0021] Example 1

[0022] A preparation method of a Centella asiatica fermentation product includes the following steps:

[0023] Step 1: Rehydrate Centella asiatica to a water content of about 30%;

[0024] Step 2: Sterilize, sterilize the rehydrated Centella asiatica;

[0025] Step 3: Inoculate Eurotium cristatum (CGMCC NO: 40145) on the rehydrated Centella asiatica, and the inoculation amount per kilogram of Centella asiatica before rehydration is 0.25 g of bacterial powder; perform aerobic fermentation at room temperature for 15 days;

[0026] Step 4: Collect the fermentation product of step 3 and dry it.

[0027] Step 5: Pulverize the dried fermentation product obtained in step 4, and subject the pulverized powder to alcohol extraction with a 95 vol% ethanol solution (the ratio of powder to ethanol solution is 1:10 (v / v)), the alcohol extraction temperature is 45 °C, the alcohol extraction time is 4 h, and then filter to obtain a filtrate, and concentrate the filtrate under reduced pressure to obtain a dry powder.

[0028] Example 2

[0029] A method for preparing a Centella asiatica fermentation product, comprising the following steps:

[0030] Step 1: Rehydrate Centella asiatica to a water content of about 30%;

[0031] Step 2: Sterilize, sterilize the rehydrated Centella asiatica;

[0032] Step 3: Inoculate Eurotium cristatum (CGMCC NO:40145) on the rehydrated Centella asiatica, and the inoculation amount is 0.1 g of bacterial powder per kilogram of Centella asiatica before rehydration; perform aerobic fermentation at room temperature for 15 days;

[0033] Step 4: Collect the fermentation product of Step 3 and dry it.

[0034] Step 5: Crush the dried fermentation product obtained in Step 4, perform alcohol extraction on the crushed powder with a 95 vol% ethanol solution (the ratio of powder to ethanol solution is 1:10 (v / v)), the alcohol extraction temperature is 40 °C, the alcohol extraction time is 5 h, then filter to obtain a filtrate, and concentrate the filtrate under reduced pressure to obtain a dry powder.

[0035] Example 3

[0036] A method for preparing a Centella asiatica fermentation product, comprising the following steps:

[0037] Step 1: Rehydrate Centella asiatica to a water content of about 30%;

[0038] Step 2: Sterilize, sterilize the rehydrated Centella asiatica;

[0039] Step 3: Inoculate Eurotium cristatum (CGMCC NO:40145) on the rehydrated Centella asiatica, and the inoculation amount is 0.5 g of bacterial powder per kilogram of Centella asiatica before rehydration; perform aerobic fermentation at room temperature for 15 days;

[0040] Step 4: Collect the fermentation product of Step 3 and dry it.

[0041] Step 5: Crush the dried fermentation product obtained in Step 4, perform alcohol extraction on the crushed powder with a 95 vol% ethanol solution (the ratio of powder to ethanol solution is 1:10 (v / v)), the alcohol extraction temperature is 50 °C, the alcohol extraction time is 3 h, then filter to obtain a filtrate, and concentrate the filtrate under reduced pressure to obtain a dry powder.

[0042] Comparative Example 1

[0043] A method for preparing a Centella asiatica extract, comprising the following steps:

[0044] Centella asiatica was pulverized, and the pulverized powder was subjected to alcohol extraction with a 95 vol% ethanol solution (the ratio of powder to ethanol solution was 1:10 (v / v)). The alcohol extraction temperature was 45 °C, and the alcohol extraction time was 4 h. Then, filtration was carried out to obtain a filtrate, and the filtrate was concentrated under reduced pressure to obtain a dry powder.

[0045] The dry powders of the above-mentioned examples and comparative examples were redissolved in a 95 vol% ethanol solution, and a Centella asiatica extract solution with a concentration of 1% was obtained after filtration.

[0046] Testing of the antioxidant efficacy of cosmetics

[0047] Test method

[0048] (1) Treatment of the control and test samples

[0049] Sample group: The above-mentioned 1% Centella asiatica extract solution was diluted 20 times with 95% ethanol;

[0050] Positive control (vitamin E, purity ≥ 96%): Diluted with 95% ethanol to a concentration of 0.1% of the positive control;

[0051] Negative control: 95% ethanol.

[0052] Test operation steps

[0053] A sample tube, a sample background tube, a DPPH tube, and a solvent background tube were set up. Three parallel tubes were set up for each group. Different reagent solutions were added to the four groups respectively, shaken gently, and left to stand at room temperature for 5 minutes. The reaction solutions of each group were transferred into 1 cm cuvettes, and the absorbance was measured at 517 nm.

[0054] Calculation formula

[0055]

[0056] In the formula: T—the absorbance of the sample tube, that is, the absorbance of the solution after the sample reacts with DPPH;

[0057] T0—the absorbance of the sample background;

[0058] C—the average value of the absorbance of the DPPH tube three times, that is, the absorbance of the DPPH solution without adding the sample;

[0059] C0—the absorbance of the solvent background.

[0060] The test results refer to Table 1;

[0061] Table 1 Detection results

[0062] DPPH Example 1 72.933 Example 2 61.257 Example 3 85.461 Comparative Example 1 45.396 Negative control -1.724 Positive control 95.345

[0063] Result analysis:

[0064] From the preliminary performance analysis of the above fermentation products, it can be seen that after fermenting Centella asiatica with Eurotium cristatum (CGMCC NO: 40145), the free radical scavenging performance of the alcohol-soluble products of Centella asiatica can be significantly improved;

[0065] The applicant simultaneously performed cell lysis on the bacterial powder of Eurotium cristatum (CGMCC NO: 40145), sent the alcohol-soluble cell lysate for testing, and measured that the free radical scavenging performance of 3% of the cell lysate was 96.932%;

[0066] The free radical scavenging performance of Examples 1 to 3 above is superior to that of Comparative Example 1, which may stem from any one of two factors: the free radical scavenging ability of the cell lysate of Eurotium cristatum and the further improvement of the free radical scavenging ability of the free radicals of Centella asiatica through fermentation.

[0067] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and without departing from the spirit or basic characteristics of the present invention, the present invention can be implemented in other specific forms. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention.

Claims

1. Use of fermented plant components by Eurotium cristatum in preparing cosmetics; The plant component is Centella asiatica; The taxonomic name of the Eurotium cristatum is Eurotium cristatum, and the preservation number is: CGMCC NO: 40145; the preservation date is March 10, 2022, and the preservation unit is the General Microbiology Center of the China Microbial Culture Collection Center.

2. A method for preparing a Centella asiatica fermentation product, characterized in that, It includes the following steps: Step 1: Rehydrate Centella asiatica to a moisture content of 30%; Step 2: Sterilize, sterilize the rehydrated Centella asiatica; Step 3: Inoculate the rehydrated Centella asiatica with the Eurotium cristatum as described in claim 1, and the inoculation amount per kilogram of Centella asiatica before rehydration is 0.1 - 0.5 g of bacterial powder; perform aerobic fermentation at room temperature for 15 days; Step 4: Collect the fermentation product of step 3 and dry it.

3. The preparation method according to claim 2, characterized in that, The dried fermentation product obtained in step 4 is pulverized, and the pulverized powder is subjected to alcohol extraction with a 95 vol% ethanol solution, then filtered to obtain a filtrate, and the filtrate is concentrated under reduced pressure to obtain a dry powder.

4. The preparation method according to claim 3, characterized in that, The temperature of the alcohol extraction is 40 to 50 °C, and the alcohol extraction time is 3 to 5 hours.

5. A centella asiatica fermentation product, characterized in that, Prepared by the method described in any one of claims 2 to 5.

Images