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Method for extracting elastin peptide

The technology of an elastin peptide and an extraction method, which is applied to the extraction field of elastin peptide, can solve the problems of complicated preparation process, low efficiency and high cost, and achieve the effects of high extraction efficiency, efficient utilization and low cost

Inactive Publication Date: 2019-04-26
FUJIAN KANG SHI MEI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] With the increasing demand for elastin peptide in the market, the existing elastin peptide preparation process is far from meeting the needs due to cumbersome preparation process, high cost, and low efficiency.

Method used

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  • Method for extracting elastin peptide
  • Method for extracting elastin peptide

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Experimental program
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Embodiment 1

[0029] A method for extracting elastin peptides, comprising the following specific steps:

[0030] (1) Remove impurities such as fish meat from 500g fish cartilage, wash and mince, add 2 times the volume of 95% ethanol solution to soak for 6 hours, remove ethanol, repeat soaking with 95% ethanol solution once, remove ethanol, wash with distilled water twice ;

[0031] (2) Add 250mL of Tris-HCl buffer solution with pH 9.0 to every 100g of washed fish cartilage, then ultrasonic frequency 60KHz, power 150W, ultrasonic treatment time 30min;

[0032] (3) Add 400mg of elastase to every 100g of ultrasonically treated cartilage solution, incubate in a constant temperature water bath at 30°C, and shake at 150r / min for 1h;

[0033] (4) Heat the water bath to 40°C, heat it for 10 minutes to inactivate the enzyme, filter the enzymolysis solution, discard the filter residue, and collect the filtrate;

[0034] (5) For the filtrate obtained in step (4), select an ultrafiltration membrane w...

Embodiment 2

[0039] A method for extracting elastin peptides, comprising the following specific steps:

[0040] (1) Remove impurities such as fish meat from 1000g fish cartilage, wash and mince, add 3 times the volume of 95% ethanol solution to soak for 6 hours, remove ethanol, repeat immersion once in 95% ethanol solution for fish cartilage, remove ethanol, and wash with distilled water 2 times;

[0041] (2) Add 300mL Tris-HCl buffer solution with pH 9.0 to every 100g of washed fish cartilage, then ultrasonic frequency 60KHz, power 150W, ultrasonic treatment time 30min;

[0042] (3) Add 500mg of elastase to every 100g of ultrasonically treated cartilage solution, incubate in a constant temperature water bath at 30°C, and shake at 150r / min for 1h;

[0043] (4) Heat the water bath to 40°C, heat it for 10 minutes to inactivate the enzyme, filter the enzymolysis solution, discard the filter residue, and collect the filtrate;

[0044] (5) For the filtrate obtained in step (4), select an ultr...

Embodiment 3

[0049] A method for extracting elastin peptides, comprising the following specific steps:

[0050] (1) Remove impurities such as fish meat from 600g fish cartilage, wash and mince, add 2.5 times the volume of 95% ethanol solution to soak for 6 hours, remove ethanol, repeat immersion once in 95% ethanol solution for fish cartilage, remove ethanol, and wash with distilled water 2 times;

[0051] (2) Add 280mL of Tris-HCl buffer solution with pH 9.0 to every 100g of washed fish cartilage, then ultrasonic frequency 60KHz, power 150W, ultrasonic treatment time 30min;

[0052] (3) Add 450mg of elastase to every 100g of ultrasonically treated cartilage solution, incubate in a constant temperature water bath at 30°C, and shake at 150r / min for 1h;

[0053] (4) Heat the water bath to 40°C, heat it for 10 minutes to inactivate the enzyme, filter the enzymolysis solution, discard the filter residue, and collect the filtrate;

[0054](5) For the filtrate obtained in step (4), select an u...

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Abstract

The invention discloses a method for extracting elastin peptide, belonging to the technical field of extraction of bioactive substances. The method comprises the steps of removing impurities such as fish meat from fish cartilage, washing and mincing, and soaking in an ethanol solution; adding a Tris-HCl buffer solution, and carrying out ultrasonic treatment; adding elastase, and performing shakingculture in a constant temperature water bath; carrying out suction filtration on enzymatic hydrolysate after enzyme deactivation, discarding filter residues, and collecting filtrate; enabling the filtrate to be subjected to primary ultrafiltration, and collecting permeate; enabling the permeate to be subjected to secondary ultrafiltration, and collecting ultrafiltrate; dialyzing and desalting theobtained ultrafiltrate, carrying out rotary evaporation, and carrying out freeze-drying to obtain the elastin polypeptide. The method for extracting the elastin peptide is simple and rapid, high in extraction rate and low in cost. The prepared elastin polypeptide is strong in reducing power, is capable of effectively scavenging free radicals, is good in antioxidant effect and can significantly increase the content of collagen peptide in the skin. The method has a wide application value in the industries of food, cosmetics and medicine.

Description

technical field [0001] The invention belongs to the technical field of bioactive substance extraction, and in particular relates to an extraction method of elastin peptide. Background technique [0002] Elastin is one of the main components of elastic fibers, which are composed of homogeneous elastin surrounded by microfibril shells. Microfibrils are composed of a number of glycoproteins that are necessary to maintain the integrity of elastic fibers. In the elastic tissue of the body's development, glycoprotein microfibrils, which are the skeleton of elastin attachment, often appear before elastin, and play an important role in the assembly of elastin molecules into elastic fibers. Elastin is a highly crossed water-insoluble hydrolyzed protein with a molecular weight of 70KDa. Non-polar amino acids (hydrophobic amino acids) account for 95% of the elastin molecule, glycine accounts for 1 / 3 of the total, proline accounts for 10%, and hydroxyproline accounts for 1%. It has an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/46C07K1/36C07K1/34C07K1/30A61K8/64A61Q19/08A61K38/39A61P39/06
CPCA61P39/06A61Q19/08A61K8/64C12P21/06C07K14/461A61K38/00
Inventor 陈国荣黄轲
Owner FUJIAN KANG SHI MEI BIOTECH CO LTD
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