Anti-aging injection and application

An anti-aging and injection technology, applied in the field of protein medicine, to achieve good wrinkle-removing effect, promote growth and differentiation, and delay skin aging

Pending Publication Date: 2020-07-10
白晋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Surgical lifting of the lower face and line techniques can effectively improve the problem of lower facial relaxation, but the target population of this operation is still for people with obvious facial aging and sagging. Although the effect is obvious, it needs to bear the trauma and possible serious possibility of complications

Method used

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  • Anti-aging injection and application
  • Anti-aging injection and application

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Experimental program
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Effect test

specific Embodiment I

[0022] Specific embodiment 1: preparation process:

[0023] 1. Purification of elastin: Suspend 10 g of human recombinant elastin powder in 100 mL of 0.1M NaOH solution, stir at 95°C for 1 hour, wash with water until neutral, discard the supernatant, lyophilize, and store at -18°C until use.

[0024] 2. Preparation of elastin hydrolyzate: disperse elastin in deionized water, elastin:water = 1:10, w / w, adjust the mixture to pH 8.0 with 1M NaOH, then add Alcalase® 2.4L, 2 %, w / w, enzymatic hydrolysis. The resulting mixture was incubated at 55 °C for 10 h with shaking, followed by a boiling water bath for 15 min, and then centrifuged at 8000 g for 20 min at 4 °C. Subsequently, the collected supernatants were lyophilized and stored at −18 °C until use.

[0025] 3. Acquisition of culture supernatant of human adipose-derived mesenchymal stem cells (hADSCs):

[0026]1) Transfer the adipose tissue obtained during the operation into a sterile large petri dish, cut it repeatedly wit...

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Abstract

The invention aims to provide an anti-aging injection. The anti-aging injection comprises elastin peptide, and is characterized by further comprising human adipose-derived mesenchymal stem cell culture medium supernatant and seaweed extract. Adipose-derived stem cells and the elastin peptide are combined and are assisted by seaweed extract liquor, synthesis of elastin can be promoted, activity ofelastase is inhibited, proliferation and differentiation of epidermal cells are promoted by the aid of vitamin C, oxygen free radicals are removed, skin repair of injured portions is promoted, growthand differentiation of skin cells are promoted by vitamin A, a hematopoietic function of surface skin is enhanced, the structure of skin elastic tissues is reconstructed essentially, and aging of human skin is delayed. The adipose-derived stem cells and the elastin are combined and are assisted by the seaweed extract liquor for facial lifting, the problems of facial sagging, non-elasticity of skinand the like caused by aging of the skin can be solved fundamentally, and a good wrinkle removing effect is achieved.

Description

technical field [0001] The invention relates to the field of protein medicine, in particular to an anti-aging injection comprising human adipose-derived mesenchymal stem cell culture supernatant. Background technique [0002] It is human nature to love beauty. Due to differences in geography, culture, history, race, etc., the standards of beauty are different in different countries and in different periods, but the expectation of staying young remains the same. Anti-aging research has a long history. In ancient Egypt, ancient Rome, ancient India, and ancient Babylon, it is still a distant dream to rejuvenate and live forever, but it is possible to freeze youth and reverse age. reality. Aging is an inescapable reality. The main reasons are photochemical damage and aging changes. Chronic sunlight exposure in daily life causes abnormal arrangement and accumulation of elastic fibers in the skin; the number of collagen fibers decreases, and the remaining fibers are arranged in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/64A61K8/67A61K8/9706A61K8/9711A61Q19/08C12P21/06C12N5/0775
CPCA61K8/64A61K8/671A61K8/676A61K8/9706A61K8/9711A61K8/99A61K2800/592A61K2800/782A61K2800/805A61K2800/91A61Q19/08C07K14/78C12N5/0667C12N2500/32C12N2500/40C12N2500/90C12N2501/11C12N2501/115C12N2501/14C12N2501/15C12N2501/39C12N2501/86C12N2501/998C12P21/06
Inventor 白晋
Owner 白晋
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