Anti-aging injection and application
An anti-aging and injection technology, applied in the field of protein medicine, to achieve good wrinkle-removing effect, promote growth and differentiation, and delay skin aging
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specific Embodiment I
[0022] Specific embodiment 1: preparation process:
[0023] 1. Purification of elastin: Suspend 10 g of human recombinant elastin powder in 100 mL of 0.1M NaOH solution, stir at 95°C for 1 hour, wash with water until neutral, discard the supernatant, lyophilize, and store at -18°C until use.
[0024] 2. Preparation of elastin hydrolyzate: disperse elastin in deionized water, elastin:water = 1:10, w / w, adjust the mixture to pH 8.0 with 1M NaOH, then add Alcalase® 2.4L, 2 %, w / w, enzymatic hydrolysis. The resulting mixture was incubated at 55 °C for 10 h with shaking, followed by a boiling water bath for 15 min, and then centrifuged at 8000 g for 20 min at 4 °C. Subsequently, the collected supernatants were lyophilized and stored at −18 °C until use.
[0025] 3. Acquisition of culture supernatant of human adipose-derived mesenchymal stem cells (hADSCs):
[0026]1) Transfer the adipose tissue obtained during the operation into a sterile large petri dish, cut it repeatedly wit...
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