Composition and application thereof in cosmetics

Abstract

The invention provides a composition and application thereof in cosmetics, and belongs to the technical field of cosmetics. The composition comprises hexapeptide and a lavender extract in a mass ratio of (10 mg): (10-1000 mg): 0.1 kg. And the amino acid composition of the hexapeptide is glycine-proline-glutamic acid-glycine-proline-glutamic acid. The composition has the effect of simultaneously increasing expression of skin collagen XVII, collagen IV, elastin, aquaporin AQP3 and nighttime protein, so that the anti-aging effect can be achieved.

Description

Technical Field

[0001] The present application relates to the technical field of cosmetics, and in particular, to a composition and its application in cosmetics. Background Art

[0002] Skin aging undergoes complex physiological and biochemical processes, such as a decrease in the renewal rate of intracellular components and intercellular matrix, a gradual reduction and loss of function of collagen fibers and elastic fibers, atrophy and dryness of the dermis and epidermis, decreased skin elasticity and firmness, fine lines and wrinkles, pigmentation or spots on the skin, etc. Factors that cause physiological and biochemical changes related to aging include endogenous factors and environmental damage. Endogenous factors include genetic inheritance, cell activity, hormones and metabolic processes, and environmental damage includes sunlight, pollution, toxins, electromagnetic radiation, etc.

[0003] It is difficult for existing anti-aging active ingredients to achieve anti-aging effects from multiple angles. They can usually only inhibit the aging phenomenon caused by one factor, but cannot take into account multiple aspects such as endogenous and environmental damage to slow down the aging of the eye skin. Summary of the invention

[0004] The present application provides a composition and its application in cosmetics, which has the effect of simultaneously increasing the expression of skin collagen XVII, collagen IV, elastin, aquaporin AQP3 and nocturnal protein.

[0005] The embodiment of the present application is implemented as follows:

[0006] In a first aspect, the present application provides an example of a composition comprising: hexapeptide and lavender extract in a mass ratio of 10 mg:10 kg to 1000 mg:0.1 kg.

[0007] The amino acid composition of the hexapeptide is glycine-proline-glutamate-glycine-proline-glutamate.

[0008] In the above technical scheme, the composition of the present application has the effect of simultaneously increasing the expression of skin collagen XVII, collagen IV, elastin, aquaporin AQP3 and nocturnal protein, thereby achieving an anti-aging effect.

[0009] In combination with the first aspect, in a first possible example of the first aspect of the present application, the mass ratio of the above-mentioned hexapeptide to the lavender extract is 100 mg:5 kg to 500 mg:1 kg.

[0010] In combination with the first aspect, in a second possible example of the first aspect of the present application, the lavender extract is Provence lavender extract.

[0011] In the above example, Provence lavender has a higher water content and is more drought-resistant than other lavender varieties.

[0012] In combination with the first aspect, in a third possible example of the first aspect of the present application, the lavender extract includes 40ppm to 50ppm of miRNA, 1g / L to 3g / L of sugar compounds and 400ppm to 900ppm of phenolic compounds.

[0013] In combination with the first aspect, in a fourth possible example of the first aspect of the present application, the length of the miRNA is 20 to 24 nucleotides.

[0014] In combination with the first aspect, in a fifth possible example of the first aspect of the present application, the lavender extract is extracted by the following method:

[0015] A chelating agent is added to a first mixture including lavender and water, and a second mixture is prepared after ultrasonic extraction at 40° C. to 50° C. for 0.5 h to 1 h, and solids in the second mixture are removed to obtain a filtrate.

[0016] In the above examples, the extraction method of the lavender extract of the present application can extract a water-soluble lavender extract that does not contain essential oil.

[0017] In combination with the first aspect, in a sixth possible example of the first aspect of the present application, the chelating agent includes tetrasodium EDTA and / or phytic acid.

[0018] In combination with the first aspect, in a seventh possible example of the first aspect of the present application, before adding the chelating agent to the first mixture, a base is used to adjust the pH value of the first mixture to 10-11.

[0019] In the above example, the use of a base is able to dissolve the acid in the lavender, which is an important component of the lavender extract.

[0020] In combination with the first aspect, in an eighth possible example of the first aspect of the present application, after removing the solid matter in the second mixture, an acid is used to adjust the pH value of the filtrate to 6-7.

[0021] In the above example, the pH value of the filtrate is adjusted to 6-7 using an acid, so that the lavender extract can be directly applied to cosmetics.

[0022] In a second aspect, the present application provides an application of the above-mentioned composition in cosmetics.

[0023] Optionally, the cosmetic product is used to combat signs of skin aging, improve skin elasticity and firmness, strengthen the skin barrier, reduce wrinkles, improve and nighttime skin repair or improve hair condition. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0025] Figure 1 The immunofluorescence image of the composition of Example 1 of the present application and the blank control group increasing the expression of AQP3 water channel protein in human primary keratinocytes;

[0026] Figure 2 This is a quantitative analysis diagram of the composition of Example 1 of the present application and the blank control group increasing the expression of AQP3 water channel protein in human primary keratinocytes;

[0027] Figure 3 This is an immunofluorescence image of the hexapeptide, lavender extract, composition and blank control group in Example 1 of the present application increasing the expression of COL XVII protein;

[0028] Figure 4 This is a quantitative analysis chart of the hexapeptide, lavender extract, composition and blank control group in Example 1 of the present application increasing the expression of COL XVII protein;

[0029] Figure 5 This is an immunofluorescence image of the hexapeptide, lavender extract, composition and blank control group in Example 1 of the present application increasing the expression of COL IV protein;

[0030] Figure 6 This is a quantitative analysis chart of the increase in elastin expression of the composition of Example 1 of the present application, the TGF-β control group and the blank control group;

[0031] Figure 7 This is a quantitative analysis chart showing the increase in nighttime protein expression in the composition of Example 1 of the present application and the blank control group. DETAILED DESCRIPTION

[0032] It is difficult for existing anti-aging active ingredients to achieve anti-aging effects from multiple angles. They can usually only inhibit the aging phenomenon caused by one factor, but cannot take into account multiple aspects such as endogenous and environmental damage to slow down the aging of the eye skin.

[0033] Collagen XVII (COL17) is an important transmembrane protein that connects the epidermis and dermis. It forms the tension structure of the epidermal junction layer (basement membrane) and plays an adhesion role. Collagen XVII is also a key molecule to maintain the normal function of hair follicle stem cells. Collagen XVII is an important focus of anti-aging and anti-hair loss cosmetics. Collagen IV fixes the epidermis to the epidermal junction layer, forming a mesh structure to stabilize the structure of the epidermal junction layer. The reduction in the content of collagen XVII and collagen IV will flatten the epidermal junction layer, causing the skin to sag and wrinkle. Elastin plays an important role in skin elasticity. Aging and ultraviolet damage will lead to a decrease in elastin, which is the main reason for aging phenomena such as sagging, sagging and wrinkles in aging skin. Aquaporin 3 (AQP3) is the most important water channel protein on human skin, which has the function of transporting small molecules such as water, glycerol and urea across the membrane. Nocturnin is closely associated with the skin's circadian rhythm and is also a key regulator of metabolism by promoting the production of more NAD+ and NADH, specific metabolites that contribute to energy production and protect cells from damage.

[0034] The inventors of the present application have discovered that a composition comprising a hexapeptide having an amino acid composition of glycine-proline-glutamate-glycine-proline-glutamate and a lavender extract has the effect of simultaneously increasing the expression of skin collagen XVII, collagen IV, elastin, aquaporin AQP3 and nocturnal protein, thereby achieving an anti-aging effect.

[0035] The composition of the present application and its application in cosmetics are described in detail below:

[0036] The present application provides a composition, comprising: hexapeptide and lavender extract in a mass ratio of 10 mg:10 kg to 1000 mg:0.1 kg.

[0037] Optionally, the mass ratio of the hexapeptide to the lavender extract in the composition is 100 mg:5 kg to 500 mg:1 kg.

[0038] Among them, the amino acid composition of the hexapeptide is glycine-proline-glutamate-glycine-proline-glutamate.

[0039] The hexapeptide can be prepared by solid phase synthesis or similar methods. The present application provides a method for preparing the hexapeptide:

[0040] With Fmoc-protected amino acid and amino resin as raw materials, 3mmol Fmoc-Gly, 3mmol 1-hydroxy-benzotriazole, 3.3mmol dicyclohexylcarbodiimide and 20mg 4-N,N'-dimethylaminopyridine, 8mL DMF and 7mL dichloromethane are added to 1mmol activated amino resin, react for 30min, wash, and vacuum dry to obtain Fmoc-Gly-resin; weigh 0.1mmol Fmoc-Gly-resin obtained, and repeat the following process: weigh 0.1mmol Fmoc-amino acid-resin, add 5mL deprotection solution (20wt% piperidine / DMF), wash, add 0.3mmol Fmoc-amino acid, react for 30min, wash, and vacuum dry; connect Fmoc-Pro, Fmoc-Gln, Fmoc-Gly, Fmoc-Pro and Fmoc-Gln in sequence. After the reaction is completed, wash and drain, add 5mL of cutting solution (75wt% trifluoroacetic acid, 15wt% ethanedithiol, 5wt% phenol and 5wt% water), stir and react for 90min, centrifuge, and purify the filtrate by reverse phase high performance liquid chromatography to obtain a hexapeptide with a purity of more than 95%, and prepare a 100ppm hexapeptide aqueous solution.

[0041] Optionally, the lavender extract is lavender of Provence extract.

[0042] The Latin name of Provence lavender is Lavandula angustifolia, its botanical name is lavender, and its common name is English lavender.

[0043] Compared with other lavender varieties, Provence lavender has a higher water content and better drought resistance.

[0044] Optionally, the lavender extract comprises 40ppm to 50ppm of miRNA, 1g / L to 3g / L of sugar compounds and 400ppm to 900ppm of phenolic compounds, and does not contain essential oil components.

[0045] Among them, the length of miRNA is 20 to 24 nucleotides.

[0046] Lavender extract is extracted by the following method:

[0047] A chelating agent is added to a first mixture including lavender and water, and a second mixture is prepared after ultrasonic extraction at 40° C. to 50° C. for 0.5 h to 1 h, and solids in the second mixture are removed.

[0048] As an example, the ultrasonic extraction temperature may be 40°C, 41°C, 42°C, 43°C, 44°C, 45°C, 46°C, 47°C, 48°C, 49°C, or 50°C.

[0049] The ultrasonic extraction time can be 0.5 h, 0.6 h, 0.7 h, 0.8 h, 0.9 h or 1 h.

[0050] Before adding the chelating agent to the first mixture, the pH value of the first mixture is adjusted to 10-11 with a base.

[0051] Using an alkali can dissolve the acid in lavender, which is an important component of lavender extract.

[0052] The mass percentage of lavender in the first mixture is 1 wt% to 10 wt%.

[0053] Chelating agents include tetrasodium EDTA and / or phytic acid.

[0054] The method of removing the solid matter in the second mixture includes centrifugation, and the centrifugation includes centrifugation at 3000-8000 rpm for 10-30 min.

[0055] After removing the solids from the second mixture, the pH of the filtrate was adjusted to 6-7 with an acid.

[0056] Acid is used to adjust the pH value of the filtrate to 6-7, so that the lavender extract can be directly applied to cosmetics.

[0057] Finally, the filtrate is filtered through a 0.2 μm filter membrane to obtain a clarified filtrate, which is the lavender extract.

[0058] The present application also provides an application of the composition in the above embodiment in cosmetics.

[0059] Optionally, the cosmetic product is used to combat signs of skin aging, improve skin elasticity and firmness, strengthen the skin barrier, reduce wrinkles, improve and nighttime skin repair or improve hair condition.

[0060] The embodiments of the present application will be described in detail below in conjunction with the examples, but it will be appreciated by those skilled in the art that the following examples are only used to illustrate the present application and should not be considered as limiting the scope of the present application. In the examples, if specific conditions are not specified, they are carried out according to normal conditions or the conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0061] Example 1

[0062] The present invention provides a composition comprising the following steps:

[0063] S1. Preparation of lavender extract

[0064] 50 g of the aerial part of Provence lavender was dried and crushed, and then prepared into a 5 wt % first mixture with 950 mL of deionized water, the pH value of the first mixture was adjusted to 10.5 with alkali, 10 mM tetrasodium EDTA was added, and ultrasonic extraction was performed at 50° C. for 1 hour to obtain a second mixture, the second mixture was centrifuged at 4000 rpm for 10 min to remove solids to obtain a filtrate, and then the pH value of the filtrate was adjusted to 6.3 with acid, and finally the filtrate was filtered with a 0.2 μm filter membrane to obtain a clarified filtrate, that is, a lavender extract without essential oil components.

[0065] S2. Preparation of hexapeptide

[0066] With Fmoc-protected amino acid and amino resin as raw materials, 3mmol Fmoc-Gly, 3mmol 1-hydroxy-benzotriazole, 3.3mmol dicyclohexylcarbodiimide and 20mg 4-N,N'-dimethylaminopyridine, 8mL DMF and 7mL dichloromethane are added to 1mmol activated amino resin, react for 30min, wash, and vacuum dry to obtain Fmoc-Gly-resin; weigh 0.1mmol Fmoc-Gly-resin obtained, and repeat the following process: weigh 0.1mmol Fmoc-amino acid-resin, add 5mL deprotection solution (20wt% piperidine / DMF), wash, add 0.3mmol Fmoc-amino acid, react for 30min, wash, and vacuum dry; connect Fmoc-Pro, Fmoc-Gln, Fmoc-Gly, Fmoc-Pro and Fmoc-Gln in sequence. After the reaction is completed, wash and drain, add 5mL of cutting solution (75wt% trifluoroacetic acid, 15wt% ethanedithiol, 5wt% phenol and 5wt% water), stir and react for 90min, centrifuge, and purify the filtrate by reverse phase high performance liquid chromatography to obtain a hexapeptide with a purity of more than 95%, and prepare a 100ppm hexapeptide aqueous solution.

[0067] S3. Preparation of composition

[0068] 100 mg of hexapeptide and 1 kg of lavender extract were mixed evenly at room temperature to prepare a composition, referred to as Osmirage.

[0069] Example 2

[0070] The present invention provides a composition comprising the following steps:

[0071] S1. Preparation of lavender extract

[0072] 50 g of the aerial part of Provence lavender was dried and crushed, and then prepared into a 5 wt % first mixture with 950 mL of deionized water, the pH value of the first mixture was adjusted to 10.5 with alkali, 10 mM tetrasodium EDTA was added, and ultrasonic extraction was performed at 50° C. for 1 hour to obtain a second mixture, the second mixture was centrifuged at 4000 rpm for 10 min to remove solids to obtain a filtrate, and then the pH value of the filtrate was adjusted to 6.3 with acid, and finally the filtrate was filtered with a 0.2 μm filter membrane to obtain a clarified filtrate, that is, a lavender extract without essential oil components.

[0073] S2. Preparation of hexapeptide

[0074] With Fmoc-protected amino acid and amino resin as raw materials, 3mmol Fmoc-Gly, 3mmol 1-hydroxy-benzotriazole, 3.3mmol dicyclohexylcarbodiimide and 20mg 4-N,N'-dimethylaminopyridine, 8mL DMF and 7mL dichloromethane are added to 1mmol activated amino resin, react for 30min, wash, and vacuum dry to obtain Fmoc-Gly-resin; weigh 0.1mmol Fmoc-Gly-resin obtained, and repeat the following process: weigh 0.1mmol Fmoc-amino acid-resin, add 5mL deprotection solution (20wt% piperidine / DMF), wash, add 0.3mmol Fmoc-amino acid, react for 30min, wash, and vacuum dry; connect Fmoc-Pro, Fmoc-Gln, Fmoc-Gly, Fmoc-Pro and Fmoc-Gln in sequence. After the reaction is completed, wash and drain, add 5mL of cutting solution (75wt% trifluoroacetic acid, 15wt% ethanedithiol, 5wt% phenol and 5wt% water), stir and react for 90min, centrifuge, and purify the filtrate by reverse phase high performance liquid chromatography to obtain a hexapeptide with a purity of more than 95%, and prepare a 100ppm hexapeptide aqueous solution.

[0075] S3. Preparation of composition

[0076] 10 mg of hexapeptide and 10 kg of lavender extract were mixed evenly at room temperature to prepare a composition.

[0077] Example 3

[0078] The present invention provides a composition comprising the following steps:

[0079] S1. Preparation of lavender extract

[0080] 50 g of the aerial part of Provence lavender was dried and crushed, and then prepared into a 5 wt % first mixture with 950 mL of deionized water, the pH value of the first mixture was adjusted to 10.5 with alkali, 10 mM tetrasodium EDTA was added, and ultrasonic extraction was performed at 50° C. for 1 hour to obtain a second mixture, the second mixture was centrifuged at 4000 rpm for 10 min to remove solids to obtain a filtrate, and then the pH value of the filtrate was adjusted to 6.3 with acid, and finally the filtrate was filtered with a 0.2 μm filter membrane to obtain a clarified filtrate, that is, a lavender extract without essential oil components.

[0081] S2. Preparation of hexapeptide

[0082] With Fmoc-protected amino acid and amino resin as raw materials, 3mmol Fmoc-Gly, 3mmol 1-hydroxy-benzotriazole, 3.3mmol dicyclohexylcarbodiimide and 20mg 4-N,N'-dimethylaminopyridine, 8mL DMF and 7mL dichloromethane are added to 1mmol activated amino resin, react for 30min, wash, and vacuum dry to obtain Fmoc-Gly-resin; weigh 0.1mmol Fmoc-Gly-resin obtained, and repeat the following process: weigh 0.1mmol Fmoc-amino acid-resin, add 5mL deprotection solution (20wt% piperidine / DMF), wash, add 0.3mmol Fmoc-amino acid, react for 30min, wash, and vacuum dry; connect Fmoc-Pro, Fmoc-Gln, Fmoc-Gly, Fmoc-Pro and Fmoc-Gln in sequence. After the reaction is completed, wash and drain, add 5mL of cutting solution (75wt% trifluoroacetic acid, 15wt% ethanedithiol, 5wt% phenol and 5wt% water), stir and react for 90min, centrifuge, and purify the filtrate by reverse phase high performance liquid chromatography to obtain a hexapeptide with a purity of more than 95%, and prepare a 100ppm hexapeptide aqueous solution.

[0083] S3. Preparation of composition

[0084] 1000 mg of hexapeptide and 0.1 kg of lavender extract were mixed evenly at room temperature to prepare a composition.

[0085] Experimental Example 1 AQP3 protein expression test

[0086] After the human primary keratinocytes were revived, they were seeded in 12-well plates with an appropriate number of cells, placed in a carbon dioxide incubator at 37°C and 5% CO2 concentration, and incubated until the cells adhered. After 4 days of culture, the cells were treated with a 0.2wt% Osmirage sample prepared in accordance with Example 1 using a complete medium, and continued to be incubated in the incubator for 24 hours. After the exposure was completed, the above active substances were discarded, the cells were washed with PBS, and then the cells were fixed with cold acetone for 15 minutes. The cell slides were taken out, blocked with BSA for 30 minutes, incubated overnight with AQP3 antibody, the primary antibody was discarded, washed with PBS, and the corresponding secondary antibodies and DAPI were added and incubated in the dark. Finally, the sealing agent was added, and the Zeiss AxioScope5 fluorescence microscope was used to take pictures under the same exposure. At the same time, the ZenPro software was used to analyze the average fluorescence intensity (average optical density / number of human primary keratinocytes) of AQP3 protein in a single cell. The results are as follows Figures 1-2 shown.

[0087] from Figure 1 It can be seen from the fluorescence images that under the same exposure time, compared with the blank control group, the treatment with 0.2wt% Osmirage in Example 1 can significantly enhance the fluorescence intensity of AQP3 protein in human primary keratinocytes, which indicates that 0.2wt% Osmirage can increase the expression of AQP3 protein in human primary keratinocytes. From the analysis results of the relative fluorescence intensity of individual cells (such as Figure 2 As shown in Figure 3 , compared with the blank control group, 0.2wt% Osmirage increased the expression of AQP3 protein by 46.99%.

[0088] Test Example 2 Collagen XVII expression test

[0089] Ex vivo skin was used to detect COL XVII (type XVII collagen). The ex vivo skin was placed in a culture medium for culture, and then 1wt% hexapeptide, 1wt% lavender extract and 1wt% Osmirage sample prepared according to Example 1 were added respectively, and the cells were incubated in an incubator for 24 to 48 hours. After the culture was completed, the tissue was fixed in an OCT fixative and stored at -80°C to form a frozen tissue block; the frozen tissue block was then sliced ​​using a freezing microtome; the cut tissue sections were added with COL XVII primary and secondary antibodies for immunofluorescence staining, and after staining, each group was photographed using a Zeiss fluorescence microscope under the same exposure and background conditions, and the fluorescence intensity of the photos was calculated and analyzed using software. The results are shown in FIG. Figures 3-4 shown.

[0090] The results showed that compared with the blank control group, the fluorescence intensity of collagen XVII in the two sample treatment groups treated with hexapeptide and Osmirage was significantly enhanced, which could increase the expression of collagen XVII by 25% and 39% respectively, while the fluorescence intensity of collagen XVII in lavender extract was basically consistent with that in the blank control group, that is, it had little effect on the expression of collagen XVII.

[0091] And by Figure 4 It can be seen that the fluorescence intensity of collagen XVII after treatment with the Osmirage sample treatment group is higher than that of collagen XVII after treatment with the hexapeptide sample and the lavender extract sample treatment group, indicating that the use of hexapeptide samples or lavender extract samples alone has little or no effect on the expression of collagen XVII, while the expression effect of collagen XVII using the Osmirage sample including hexapeptide and lavender extract is significantly improved, indicating that the synergistic cooperation of hexapeptide and lavender extract increases the expression effect of collagen XVII.

[0092] Test Example 3 Collagen IV expression test

[0093] The expression of type IV collagen was detected using ex vivo skin. The ex vivo skin was placed in a culture medium for culture, and then 1wt% Osmirage, 1wt% hexapeptide and 1wt% lavender extract samples prepared in Example 1 were added respectively, and the cells were incubated in an incubator for 48 hours. After the culture was completed, the tissue was fixed in a formalin solution, and after successful fixation, it was placed in an embedding box and covered with water; then it was embedded in paraffin, and after cooling and solidifying into blocks, a paraffin slicer was used to slice the tissue; the cut tissue slices were stained with COL IV related antibodies and dyes, and then each group was observed and photographed using a Zeiss fluorescence microscope. The results are shown in the figure. Figure 5 shown.

[0094] Depend on Figure 5It can be seen that compared with the blank control group, the fluorescence intensity of type IV collagen at the true epidermal junction was enhanced after treatment with 1wt% Osmirage, 1wt% hexapeptide and 1wt% lavender extract samples. The expression of type IV collagen from strong to weak is: 1wt% Osmirage, 1wt% hexapeptide, 1wt% lavender extract, and the fluorescence intensity of collagen IV after treatment with 1wt% Osmirage sample is higher than that of collagen IV after treatment with 1wt% hexapeptide sample and 1wt% lavender extract sample, indicating that the effect of using only hexapeptide sample or lavender extract sample on the expression effect of collagen IV is low, while the expression effect of collagen IV of Osmirage sample including hexapeptide and lavender extract is significantly improved, indicating that hexapeptide and lavender extract cooperate to increase the expression effect of collagen IV.

[0095] Test Example 4 Elastin expression test

[0096] Ex vivo skin was used to detect elastin expression. The ex vivo skin was placed in a culture medium for culture, and then a 1 wt% Osmirage sample prepared in Example 1 or a positive control 50 ng / mL TGF-β was added, and the cells were incubated in an incubator for 24 hours. At the same time, 5 J / cm 2 UVA and 200mJ / cm 2 UVB irradiation was performed twice. After the culture was completed, the tissues were fixed in 10% formalin fixative and stored at -80°C to form frozen tissue blocks. The frozen tissue blocks were then sliced ​​using a freezing microtome. The sliced ​​tissue sections were added with primary and secondary antibodies for elastin for immunofluorescence staining. After staining, each group was photographed using a fluorescence microscope under the same exposure and background conditions, and the fluorescence intensity of the photos was calculated and analyzed using software. The results are shown in the figure. Figure 6 shown.

[0097] The results showed that compared with the blank control group and the positive control group, the fluorescence intensity of elastin was significantly enhanced after treatment with 1wt% Osmirage samples, which could increase the expression of elastin by 32%, which was higher than the blank control group and the positive control group of 50ng / mL TGF-β.

[0098] Experimental Example 5 Nighttime protein expression test

[0099] Resuscitated human primary keratinocytes were seeded in 12-well plates with a certain number of cells, and incubated in a carbon dioxide incubator at 37°C and 5% CO2 concentration until the cells adhered to the wall. After 4 days of culture, the cells were treated with 0.2wt% Osmirage prepared in Example 1 in complete culture medium, and continued to be incubated in the incubator for 24 hours. After the exposure is over, the cells were washed with PBS, and then fixed with cold acetone for 15 minutes. The cell slides were taken out, blocked with BSA for 30 minutes, incubated overnight with Nocturnin antibody, the primary antibody was discarded, washed with PBS, and the corresponding secondary antibodies and DAPI were added and incubated in the dark. Finally, the sealing agent was added, and the Zeiss AxioScope 5 fluorescence microscope was used to take pictures under the same exposure, and the ZenPro software was used to analyze the average fluorescence intensity of Nocturnin protein in a single cell (average optical density / number of human primary keratinocytes). The results are as follows Figure 7 shown.

[0100] The results showed that compared with the blank control group and the positive control group, the fluorescence intensity of the nocturnal protein was significantly enhanced after treatment with 0.2wt% Osmirage samples. Compared with the control group, the concentration of 0.2wt% Osmirage increased the expression of nocturnal protein by 26.41%, and the difference was significant.

[0101] The above description is only a specific embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A composition, characterized in that The composition comprises: hexapeptide and lavender extract in a mass ratio of 10 mg:10 kg to 1000 mg:0.1 kg; The amino acid composition of the hexapeptide is glycine-proline-glutamic acid-glycine-proline-glutamic acid.

2. The composition according to claim 1, characterized in that The mass ratio of the hexapeptide to the lavender extract is 100 mg:5 kg to 500 mg:1 kg.

3. The composition according to claim 1, characterized in that The lavender extract is Provence lavender extract.

4. The composition according to claim 1, characterized in that The lavender extract comprises 40ppm-50ppm of miRNA, 1g / L-3g / L of sugar compounds and 400ppm-900ppm of phenolic compounds.

5. The composition according to claim 4, characterized in that The length of the miRNA is 20 to 24 nucleotides.

6. The composition according to claim 1, characterized in that The lavender extract is extracted by the following method: A chelating agent is added to a first mixture including lavender and water, and ultrasonic extraction is performed at 40° C. to 50° C. for 0.5 h to 1 h to obtain a second mixture, and solids in the second mixture are removed to obtain a filtrate.

7. The composition according to claim 6, characterized in that The chelating agents include tetrasodium EDTA and / or phytic acid.

8. The composition according to claim 6, characterized in that Before adding the chelating agent to the first mixture, the pH value of the first mixture is adjusted to 10-11 with a base.

9. The composition according to claim 6, characterized in that After removing the solids in the second mixture, the pH value of the filtrate is adjusted to 6-7 with an acid.

10. Use of the composition according to any one of claims 1 to 9 in cosmetics; Optionally, the cosmetic is used to combat signs of skin aging, improve skin elasticity and firmness, strengthen the skin barrier, reduce wrinkles, improve and nighttime skin repair, or improve hair condition.

Images