Method for extracting suaeda salsa flavone and measuring content of flavones in suaeda salsa

Abstract

The invention relates to a method for extracting suaeda salsa flavone and measuring content of flavone in suaeda salsa. The method comprises the following steps: 1), collecting the suaeda salsa, drying the suaeda salsa and crushing the suaeda salsa; with ethanol as an extractant, soaking the suadeda salsa and carrying out microwave-assisted extracting; filtering; extracting with petroleum ether; transferring the ethanol layer to a macroporous adsorption resin layer; and eluting with water and ethanol aqueous solution sequentially; collecting the eluant, and pressurizing and concentrating the eluant to obtain flavone compound extract; 2), with rutin as a contrast, preparing standard solution with different concentration gradients; measuring the chromatographic peak area at the wavelength of 360nm through a high efficiency liquid chromatography to form flavone content contrast reference; measuring the chromatographic peak area of the to-be-measured solution; obtaining the flavone content of the to-be-measured solution according to the flavone content contrast reference; and obtaining the content of the flavone in the suaeda salsa according to the dilution multiple of the to-be-measured solution. According to the method provided by the invention, soaking and microwave-assisted extracting are adopted to extract flavone, and the content of the flavone is measured by chromatography, so that the method is simple and the solvent is easily available. Besides, the obtained flavone is high in content, and has the physiological and pharmacological activities such as antiviral activity, antioxidant activity, anti-ageing activity and the like.

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