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Method for preparing total saponins and polysaccharides from Apios americana flowers simultaneously, and application of total saponins and polysaccharides

A technology of total flower saponins and vietnamese taro, which is applied in the field of plant extraction, can solve the problems of undiscovered vietnamese saponins and polysaccharide extraction methods and uses, failure to develop and utilize resources, waste of resources, etc., and achieve strong activity, low cost, and raw materials. The effect of high utilization

Inactive Publication Date: 2013-04-17
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, there is no relevant report on the extraction method and application of vietnamese taro saponins and polysaccharides
In addition, the Dai Viet bean taro flower is basically in a state of abandonment and has not been developed and utilized, resulting in a waste of resources
[0003] At present, there are reports on the simultaneous extraction of saponins and polysaccharides from other plants, such as Chinese patent (application number: 201110029129.1, application publication number CN102178720A) discloses a method for simultaneously preparing total saponins and polysaccharides from Gynostemma pentaphyllum, but there is Deficiency in low purity

Method used

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  • Method for preparing total saponins and polysaccharides from Apios americana flowers simultaneously, and application of total saponins and polysaccharides
  • Method for preparing total saponins and polysaccharides from Apios americana flowers simultaneously, and application of total saponins and polysaccharides
  • Method for preparing total saponins and polysaccharides from Apios americana flowers simultaneously, and application of total saponins and polysaccharides

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Optimization of extraction process of total saponins from Taro flower of Da Viet bean:

[0024] The taro flowers were destemmed and cleaned, freeze-dried, and stored at low temperature; before extraction, the actual water content in the flowers was measured with a rapid moisture analyzer, and the saponin content and antioxidant properties of the samples were converted into flower dry basis for calculation. Technological process: bean taro flower powder→reflux extraction under certain conditions→filter while hot→wash residue→constant volume→analysis test.

[0025] The assay method of total saponin content is as follows:

[0026] Accurately weigh 2.5 mg of ursolic acid reference substance, add absolute ethanol to make up to 10 mL, and obtain 250 μg / mL ursolic acid standard solution. Precisely draw 0, 100, 150, 200, 250, 300, 350, and 400 μl of the reference solution into a 5mL volumetric flask, evaporate the solvent, and add 200 μl of newly prepared 5% vanillin-glacial a...

Embodiment 2

[0031] Optimization of the extraction process of taro polysaccharide from Dai Viet bean:

[0032] The taro flower was destemmed and then freeze-dried and stored at low temperature; before extraction, the actual water content in the flower was measured with a rapid moisture analyzer, and the polysaccharide yield of the sample was converted into flower dry basis for calculation. Process flow: bean taro flower powder → 90% ethanol reflux extraction → hot filtration → filter residue evaporated to dry solvent → distilled water reflux extraction → hot filtration → supernatant concentrated to a certain volume → 4°C ethanol precipitation → 5000r / min centrifugation → precipitation Add water to dissolve the compound → dialysis → obtain crude polysaccharide analysis test.

[0033] The calculation method of polysaccharide yield is as follows:

[0034] Polysaccharide yield (%) = crude polysaccharide weight / dry basis weight of Da Viet bean taro flower × 100%

[0035] Table 2 shows the d...

Embodiment 3

[0039] The simultaneous preparation method of total saponins and polysaccharides of Da Viet bean taro flower comprises the following steps:

[0040] (1) The taro flowers picked in June and July, washed, drained, freeze-dried, crushed, and passed through a 40-mesh sieve;

[0041] (2) Add 200mL of 90% ethanol solution to 10g of bean taro flower powder, reflux extraction in a 70°C water bath for 3 times, each time for 2 hours, suction filter while it is hot, wash the residue with 90% ethanol, and get the above Clear liquid and filter residue;

[0042] (3) After drying the supernatant with a rotary evaporator, dilute to 100mL with water. After degreasing an equal volume of petroleum ether, extract three times with an equal volume of water-saturated n-butanol, take the fraction of n-butanol, concentrate it with a rotary evaporator, dry it in vacuum, dissolve it in water, and apply macroporous adsorption resin AB-8 (Shanghai Mosu Scientific Equipment Co., Ltd., the same below), 75...

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Abstract

The invention discloses a method for preparing total saponins and polysaccharides from Apios americana flowers simultaneously and relates to the technical field of plant extraction. The method comprises the following steps: (1) harvesting raw materials, cleaning and drying; (2) reflux-extracting with ethanol, and filtering to obtain a supernatant and filter residues; (3) defatting the supernatant with petroleum ether, extracting with n-butanol, performing adsorption and elution, concentrating, and drying to obtain powdery Apios americana flower saponins; (4) removing the solvent from the filter residues, reflux-extracting with water, filtering, collecting and mixing the filtrates; and (5) decolorizing the filtrate, performing deproteinization, concentrating, precipitating with alcohols, dialyzing and concentrating, and drying to obtain Apios americana flower polysaccharides. The method has the advantages of simple process, high yield and low cost. Besides, the total saponins and polysaccharides of Apios americana flowers are prepared simultaneously, have high purity and strong activity, and can be widely used in pharmaceuticals or functional foods with antioxidant and blood sugar regulating effects.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for simultaneously preparing saponins and polysaccharides from taro flowers of Da Viet bean and its application. Background technique [0002] Da Viet Taro ( Apios americana Medikus) is a perennial leguminous plant, also known as Potato bean, American ground nut, twining vines, 5-7 leaflets and one compound leaf, purple flowers, butterfly corolla, native to the western United States, underground tubers are used as asexual reproduction or edible, It is a natural high-protein, high-calcium food. Due to its extremely high nutritional and health effects, it was introduced and developed by the Japanese government in the 1960s, and was widely used in various foods or food ingredients. It has become a well-known health food in Japan, mainly for modern lifestyle diseases (such as high blood pressure) , diabetes, obesity, osteoporosis, etc.) have obvious improvement e...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61K31/715A61P39/06A61P3/10A23L1/30C08B37/00
Inventor 倪勤学张有做舒恩成许光治高前欣杨冬冬
Owner ZHEJIANG FORESTRY UNIVERSITY
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