Extraction method of microalga grease

A microalgae oil and extraction method technology, applied in the direction of fat oil/fat production, fat production, etc., can solve the problem of limited effect of alcohol dissolution, and achieve the effect of facilitating crushing, improving the effect of physical crushing, and improving the efficiency of alcohol dissolution

Inactive Publication Date: 2013-04-17
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, a short-chain alcohol solvent is added after the microalgae are subjected to microwave treatment to destroy the cells. Although the short-chain alcohol solvent has a certain dissolution effect on the cell membrane phospholipid layer after the cell destruction, the effect of alcohol dissolution is limited, and the short-chain alcohol Mainly involved in base-catalyzed reactions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A kind of chlorella vulgaris was selected and cultured in a 10L tank for 15 days. At the later stage of microalgae culture, according to the established relationship between OD and cell dry weight, the concentration of microalgae was calculated to be 1.02g / L (dry weight count).

[0024] (1) Collection of microalgae cells: Take 1L of microalgae culture solution, adjust the pH of the microalgae culture solution to 12 with NaOH, let it stand for 30 minutes, take the algae solution from the bottom of the culture tank for centrifugation, control the centrifugal speed at 5000rpm, and centrifuge for 5 minutes;

[0025] (2) Breaking of microalgae cells: Take algae mud after centrifugation, add 20ml of methanol to it, oscillate and mix, and process with ultrasound, the frequency of ultrasound is 40KHz, the power of ultrasound is 400W, and the reaction time is 10min;

[0026] (3) Oil extraction: Add 120ml of n-hexane to the microalgae cell disruption liquid, shake and mix well, p...

Embodiment 2

[0029] A kind of botrytis (Botryococcus braunii) was selected and cultured in a 10L tank for 20 days. At the later stage of microalgae culture, according to the established relationship between OD and cell dry weight, the concentration of microalgae was calculated to be 1.41g / L (dry weight basis ).

[0030] (1) Collection of microalgae cells: Take 1.5L of microalgae culture solution, adjust the pH of the microalgae culture solution to 12 with NaOH, let it stand for 30 minutes, take the algae solution from the bottom of the culture tank for centrifugation, the centrifugal speed is 10000rpm, and the centrifugation time is 10min ;

[0031] (2) Breaking of microalgae cells: Take algae mud after centrifugation, add 30ml of ethanol to it, oscillate and mix, and process with ultrasound, the frequency of ultrasound is 40KHz, the power of ultrasound is 300W, and the reaction time is 15min;

[0032] (3) Oil extraction: Add 120ml of n-hexane to the microalgae cell disruption solution, s...

Embodiment 3

[0035] The algae culture liquid in Example 1 was selected for use, and the method of collecting microalgae cells was exactly the same as that in Example 1.

[0036] (1) Breaking of microalgae cells: take algae mud after centrifugation, add 30ml of ethanol to it, oscillate and mix, and treat with microwave, microwave frequency is 915MKHz, microwave power is 200W, and reaction time is 3min;

[0037](2) Oil extraction: Add 120ml of n-hexane to the microalgae cell disruption solution, shake and mix well, put it into the extraction device, set the temperature of the water bath to 80°C, and carry out the reaction for 7 hours;

[0038] (3) Solvent recovery: After the extraction, cool to room temperature and let it stand for 15 minutes. Finally, the liquid in the reactor is divided into three layers, namely oil phase, water phase and precipitation. After separation, the calculated oil yield is 23.2%.

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Abstract

The invention discloses an extraction method of microalga grease. The extraction method comprises the following steps of: (1) microalga cell collection: adjusting the pH value of a microalga culture solution to 11-12 at the later period of microalga cultivation, and collecting microalga cells in a centrifugal way; (2) microalga cell crushing: adding a C1-C6 short-chain alcohol solvent into microalga ooze obtained in the step (1), shaking and mixing the microalgae to be uniform, and then, carrying out cell breaking treatment on the microalgae; (3) grease extraction: adding one or more of organic solvents such as n-hexane and petroleum ether into a microalga cell breaking solution obtained in the step (2), and extracting the grease component in the cell breaking solution by using the organic solvents; and (4) solvent recovery: after the extraction reaction is ended, cooling the solvent to room temperature, standing for 10-30min, respectively extracting an organic solvent layer (oil phase) and a water layer (water phase), and recovering the organic solvents in the oil phase in a distillation way, and meanwhile, obtaining the microalga grease. The method has the advantages of simple operation process, short extraction time, low energy consumption, high microalga grease yield and the like and is suitable for directly extracting grease from alga ooze.

Description

technical field [0001] The invention relates to a method for purifying microalgae oil. Background technique [0002] Microalgae are a class of lower plants that grow in water and are diverse and widely distributed. This type of organism has an efficient photosynthetic reaction system, which can 2 Light energy is converted into chemical energy and stored in the body cells in the form of organic matter such as oil or starch. With the pressure of shortage of resources in human society and the increasingly serious environmental problems, the development of biodiesel and some fossil energy substitutes using microalgae has become a research hotspot. [0003] The use of microalgae for biodiesel production is a complex system engineering, covering multiple technical links, including screening and cultivation of microalgae species, large-scale cultivation of microalgae and induction of oil production, collection and processing of oil, etc. The research on microalgae as biodiesel r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B1/04
Inventor 张霖师文静李晓姝王领民
Owner CHINA PETROLEUM & CHEM CORP
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