54 results about "Cell destruction" patented technology

An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

This invention describes unique treatment methods and innovative articles that can be placed in a human or animal body to enable controlled destruction of diseased tissue. The methods include destruction of diseased cells and tissues by magnetically controlled motion and an externally controllable drug delivery process with a capability to start and stop the drug delivery at any time, for any duration. This invention provides two approaches to diseased cell destruction, (1) magneto-mechanical disturbance of cell structure (e.g. cancer cells) for cell lysis and (2) magnetically activated drug release at local regions (e.g. tumors) from a magnetic-particle-containing drug reservoir. The invention also provides combinations of both the above treatments for dual therapy. It further combines one or both of the treatments with magnetic hyperthermia for multifunctional cell destruction therapy. The approaches can be combined with magnetic MRI for monitoring the accuracy of placement as well as for following up the cancer destruction progress and appropriate reprogramming of the magneto-mechanical therapy and remote-controlled drug release.

The present invention provides systems, devices and related methods for applying electric fields for cancerous cell destruction and tissue ablation. A method can include advancing an ablation probe into a patient; deploying a guide from the delivery member along a guide tissue penetration path, deploying an electrode from the guide along an electrode tissue penetration path, and establishing current flow within the target tissue.

Disclosed is an antibody against a secreted form of GPC3 capable of detecting a secreted form of glypican 3 (GPC3) in a test sample. It is possible to determine whether a subject suffers from cancer, in particular hepatoma. Also disclosed is an antibody against GPC as well as a cell disrupting agent and an anti-cancer agent comprising the same, which can disrupt cells, in particular cancer cells.

Method and apparatus for the ionization of living cells where an optical device (14) delivers an optical pulse having an optical field power which is modified locally by an optical field power modifying means (18) to effect ionization and destruction of living cells (16).

The invention provides processes and compositions for enhancing the immunogenicity of TAP-1 expression-deficient cells by increasing the presentation of MHC Class I surface molecules for detection by cytotoxic T-lymphocyte cells through increased TAP-1 expression, which comprises administering to the TAP-1 expression-deficient cells a TAP-1 expression increasing amount of a bio-acceptable substance that promotes transcription of TAP-1 gene in the cells to cause enhanced MHC Class I surface expression of the cells. The bio-acceptable substance may be a histone H3 deacetylase inhibitor, such as trichostatin A, a transcriptional co-activator having intrinsic histone acetyl transferase activity or a histone acetyl transferase comprising at least one member of the CBP / p300 protein family. The process and compositions increase the immunogenicity of the target cells to enhance their destruction by cytotoxic lymphocytes.

Method and apparatus for the ionization of living cells where an optical device (14) delivers an optical pulse having an optical field power which is modified locally by an optical field power modifying means (18) to effect ionization and destruction of living cells (16).

A neoplasm cell destruction device utilizing low frequency sound waves to disrupt or displace cellular materials in neoplastic cells so as to damage and ultimately destruct the neoplastic cells without destructing surrounding healthy cells and thereby eliminating the need for target finding apparatus. The device includes a plurality of signal generators, a controller, a plurality of amplifiers, a plurality of transducers, and a target interface. The controller is in electrical communication with, and generates timing and control signals for simultaneously activating, the plurality of signal generators. Each amplifier is in electrical communication with a respective signal generator and amplifies the signal generated by the respective signal generator so as to form an amplified signal. Each transducer is in electrical communication with a respective amplifier and is driven by the amplified signal formed by the respective amplifier. The target interface combines the waveform formed by the plurality of transducers to form an interference wave which is a low frequency sound wave.

The Low Temperature Hyperthermia System illuminates nano-particles, which are implanted in a living organism at the locus of the cancer or into the cancer cells, with a precisely determined energy field. This energy field ensures that the optimal cancer cell and cancer stem cell destruction temperature of 42° C. is not exceeded in the tissue, which minimizes the release of Heat Shock Proteins and cancer stem cells. The Low Temperature Hyperthermia System uses specially designed nano-particles that exhibit a specific temperature rise in a given illumination energy field and then have no further temperature rise even if the applied illumination energy field increases beyond the optimal level. Alternatively, the nano-particles exhibit a tightly controlled temperature rise based on a pre-determined illumination energy field strength. This innovative approach can also use radiation and / or chemotherapy in conjunction with the nano-particle illumination to kill the majority of the cancer cells.

The invention discloses a novel wheat antioxidant peptide and a preparation method thereof, belonging to the technical field of fine and further processing of agricultural products. According to the preparation method disclosed by the invention, a novel wheat antioxidant peptide product with cytoprotection activity is obtained through acid protease hydrolysis, different cut-off molecular weight membrane filtration, gel chromatography column separation, reverse high-efficiency liquid chromatography purification, and the like by taking wheat gluten protein (wheat gluten) as a raw material. The novel wheat antioxidant peptide disclosed by the invention has relative molecular weight in the range of 1746 Da and the structure of serine-glycine-alanine-aspartate-lysine-lysine-proline-isoleucine-lysine-alanine-asparagine-histidine; in addition, the product prepared by adopting the preparation method disclosed by the invention has high purity and higher free radical scavenging capacity, can be used for effectively repairing cell destruction and tissue damage and has great application prospect in cosmetics and functional foods or medicaments.

Disclosed are Drug Delivery Molecules (DDMs) which both facilitate functional imaging, as by PET or SPECT, and create a biological effect and methods of their use. These DDMs are variously designed to target specific receptors, then become internalized and then function biologically, as for purposes of cell destruction or therapy.

The invention discloses a combined conditioning agent for sludge deep dewatering and mud cake stabilization and an application of the combined conditioning agent, and belongs to the technical field ofsludge treatment. The combined conditioning agent comprises a cell destructing reagent, a skeleton construct, and a conditioning agent for changing a floc structure of sludge; the cell destructing reagent is preferably a hypochlorite or chlorine gas, hypochlorous acid and derivatives of the hypochlorous acid produced in the conditioning process can penetrate an extracellular polymer of the sludgeand cell walls and oxidize intracellular matter, so that cells in the sludge are destructed, and harmful bacteria such as parasitic eggs and pathogens in the sludge are inactivated; coagulation and / or flocculation of the sludge by a coagulant and / or flocculant can further release extracellular and intracellular bound water of the sludge; and as an inert substance, the skeleton construct can maintain a water outlet channel of water in the sludge pressure filtration process, so that the sludge intracellular water released by the cell destruction can be quickly removed. The pretreatment method for the sludge provided by the invention can simultaneously achieve the dual purposes of deep dewatering of the sludge and inactivation of the harmful bacteria in the sludge.

A neoplasm cell destruction device. The device includes at least one signal generator and at least one transducer. The at least one transducer is driven by the at least one signal generator, and produces sound waves. The sound waves are impacted upon a neoplastic target to damage, and ultimately destruct, the neoplastic target.

Disclosed are Drug Delivery Molecules (DDMs) which both facilitate functional imaging, as by PET, MRI or SPECT, and create a biological effect and methods of their use. These DDMs which are variously designed to target specific receptors, internalized and then function biologically, as for purposes of cell destruction or therapy.

Disclosed are Drug Delivery Molecules (DDMs) which both facilitate functional imaging, as by PET, MRI or SPECT, and create a biological effect and methods of their use. These DDMs which are variously designed to target specific receptors, internalized and then function biologically, as for purposes of cell destruction or therapy.

The invention discloses golden eucommia black tea and a preparation method thereof. The method comprises the steps of 1, withering, wherein 20-30 parts of eucommia tender leaves and 60-70 parts of chimonanthus salicifolius tender leaves are mixed and then withered; 2, enzymolysis, wherein a 5-10% enzymatic hydrolysis solution is added into the withered leaves for enzymolysis for 1-3 h; 3, twisting, wherein the cell destruction rate is more than 90%, and liquid tea overflows and does not drip; 4, fermentation, wherein oxygen is introduced into the twisted tea leaves for fermentation, so that green grass fragrance disappears, the specific fragrance of the golden eucommia black tea appears, and the leaf color is dark red; 5, drying, wherein the fermented tea leaves are fed into a dryer to be dried till the water content is 5-6%. The obtained golden eucommia black tea is dark red, full in shape, attractive in appearance and highly permeable in fragrance, the soaked tea soup is mellow in taste, the fragrance is rich, no acid odor or bitter taste exists, the specific flavor of the golden eucommia black tea is achieved, and the golden eucommia black tea has the better auxiliary effect on treating high blood pressure and high blood fat.

The present invention lies in the technical field of antibody therapies involving a mechanism of target-cell destruction by ADCC. It relates to purified antibody compositions, obtained by chromatographic fractionation of the various charge isoforms naturally present in an antibody composition and combining one or more chromatographic fractions corresponding to the predominant peak of the chromatogram, the resulting monoclonal antibody composition being enriched in said predominant peak, said peak representing at least 85% of the chromatogram of the composition obtained, for use as a medicament.

A neoplasm cell destruction device utilizing low frequency sound waves to disrupt or displace cellular materials in neoplastic cells so as to damage and ultimately destruct the neoplastic cells. The device includes a plurality of signal generators, a controller, a plurality of amplifiers, a plurality of transducers, and a target interface. The controller is in electrical communication with, and generates timing and control signals for simultaneously activating, the plurality of signal generators. Each amplifier is in electrical communication with a respective signal generator and amplifies the signal generated by the respective signal generator so as to form an amplified signal. Each transducer is in electrical communication with a respective amplifier and is driven by the amplified signal formed by the respective amplifier. The target interface combines the waveforms formed by the plurality of transducers to form an interference wave that is a low frequency sound wave.

The present invention provides systems, devices and related methods for applying electric fields for cancerous cell destruction and tissue ablation. A method can include advancing an ablation probe into a patient; deploying a guide from the delivery member along a guide tissue penetration path, deploying an electrode from the guide along an electrode tissue penetration path, and establishing current flow within the target tissue.

The present invention relates to pyrimidinones and pyridones and derivatives thereof, and pharmaceutically acceptable salts thereof. Also included is a method of treatment of inflammation, rheumatoid arthritis, Pagets disease, osteoporosis, multiple myeloma, uveititis, acute or chronic myelogenous leukemia, pancreatic β cell destruction, osteoarthritis, rheumatoid spondylitis, gouty arthritis, inflammatory bowel disease, adult respiratory distress syndrome (ARDS), psoriasis, Crohn's disease, allergic rhinitis, ulcerative colitis, anaphylaxis, contact dermatitis, asthma, muscle degeneration, cachexia, Reiter's syndrome, type I diabetes, type II diabetes, bone resorption diseases, graft vs. host reaction, Alzheimer's disease, stroke, myocardial infarction, ischemia reperfusion injury, atherosclerosis, brain trauma, multiple sclerosis, cerebral malaria, sepsis, septic shock, toxic shock syndrome, fever, myalgias due to HIV-1, HIV-2, HIV-3, cytomegalovirus (CMV), influenza, adenovirus, the herpes viruses or herpes zoster infection in a mammal comprising administering an effective amount a compound as described above.

The invention discloses golden dendrobium officinale black tea and a preparation method thereof. The method comprises the steps of 1, withering, wherein 18-28 parts of dendrobium officinale tender leaves and 60-70 parts of chimonanthus salicifolius tender leaves are mixed and then withered; 2, enzymolysis, wherein a 5-10% enzymatic hydrolysis solution is added into the withered leaves for enzymolysis for 1-3 h; 3, twisting, wherein the cell destruction rate is more than 90%, and liquid tea overflows and does not drip; 4, fermentation, wherein oxygen is introduced into the twisted tea leaves for fermentation, so that green grass fragrance disappears, the specific fragrance of the golden dendrobium officinale black tea appears, and the leaf color is dark red; 5, drying, wherein the fermented tea leaves are fed into a dryer to be dried till the water content is 5-6%. The obtained golden eucommia black tea is dark red, full in shape, attractive in appearance and high permeable in fragrance, the soaked tea soup is mellow in taste, the fragrance is rich, no acid odor or bitter taste exists, the specific flavor of the golden dendrobium officinale black tea is achieved, and the golden dendrobium officinaleeucommia black tea has the antitumor function and the health care function of improving immunity.

A high efficiency preparation technique of high-end manual old fir ball tea or a tea cake of Wuyi rock tea is characterized by comprising the following technical steps: (1) withering, (2) fine manipulation, (3) stalk cutting, (4) roasting, (4) rolling, (5) pressing and (6) baking, wherein a withering method of the (1) withering procedure comprises the step of performing sunlight withering on green old fir tea, or uniformly spreading the green old fir tea on a withering trough for heating withering; the (2) fine manipulation procedure is accomplished on the withering trough; a fermentation and standing process is carried out according to the conventional technology of the Wuyi rock tea; a method of achieving partial cell damaging of green old fir tea leaves to promote enzymatic oxidation comprises the steps of uniformly spreading the green old fir tea on the withering trough, manually grabbing a needle plate to horizontally press the green old fir tea on the withering trough, forming uniformly-distributed fine needle holes in the green old fir tea leaves to achieve the partial cell damaging of the green old fir tea leaves to promote the enzymatic oxidation; the needle plate is in a structure that stainless steel needles with sharp heads are orderly arranged on a support plate and a handle is arranged on the back of the support plate; the (3) stalk cutting procedure is to manually cut off stalks of the green old fir tea after the fine manipulation procedure is accomplished.

An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

There is provided a method for preventing disruption and aggregation of stem cells. In addition, there is provided a stem cell composition for intravascular administration, in which stem cells are suspended in a solution containing aspirin. Further, there is provided a composition for preventing disruption or aggregation of stem cells, in which stem cells are suspended in a solution containing aspirin.According to the present invention, disruption and aggregation of cells may be prevented during transport or storage, such that the administered stem cells may stably reach the target tissue and exhibit the activity thereof in a more efficient manner, thereby remarkably increasing the effect of cell therapy using stem cells.

The high-efficiency production process of Wuyi rock tea handmade high-end cinnamon ball tea or tea cake is characterized in that the process steps include: (1) withering, (2) greening, (3) cutting stems, (4) frying greens, ( 4) Kneading, (5) pressing (6) baking; the (2) greening process is completed on the withering tank, and the fermentation and standing process is carried out according to the conventional process of Wuyi rock tea, which achieves the destruction of some cells of the green leaves of cinnamon tea, thereby The method to promote enzymatic oxidation is: spread the cinnamon tea greens evenly on the withering tank. During the greening process, manually grasp the needle board and press the cinnamon tea greens on the withering tank horizontally to form an even distribution on the leaves of the cinnamon tea greens. The tiny needle holes destroy some cells of the cinnamon tea green leaves, thereby promoting the enzymatic oxidation; the structure of the acupuncture board is: the stainless steel needles with sharp heads are arranged in an orderly manner on the supporting board, and the back of the supporting board is equipped with A handle constitutes the needle plate.

The present invention relates to a recombinant porcine circovirus (PCV2) subunit vaccine using yeast whole cells or lysates thereof and a method for manufacturing the same. The yeast whole cells or lysates thereof according to the present invention have an excellent effect as a vaccine composition, and can also significantly simplify processes, such as cell disruption, antigen extraction, purification, stabilization, and the like, which cannot be avoided in a procedure for manufacturing a porcine circovirus vaccine using various advantages of yeast and recombinant microorganisms.

The present invention discloses gold eucommia ulmoides black tea and a preparation method thereof. The method comprises the following steps: (1) withering is conducted: 20-30 parts of young leaves ofeucommia ulmoides and 60-70 parts of young leaves of chimonanthus salicifolius are mixed, and the mixture is withered; (2) enzyme digestion is conducted: 5-10% of enzymatic hydrolysate is added into the withered leaves to be enzyme-digested for 1-3 hours; (3) kneading is conducted: cell destruction rate is 90% or more and tea juice is overflowing without dripping; (4) fermentation is conducted: the kneaded tea leaves are subjected to an oxygen fermentation to enable green grass smell to disappear and form unique aroma of the gold eucommia ulmoides black tea, and leaf color is dark red; and (5)drying is conducted: the fermented tea leaves are sent into a dryer to be dried to a water content of 5-6%. The obtained gold eucommia ulmoides black tea is dark red, full in shape, beautiful in appearance, and highly penetrating in aroma. Brewed tea soup is mellow in mouthfeel, rich in aroma, and free of any astringent and bitter taste, and has unique flavor of the gold eucommia ulmoides black tea. Besides, the product has both relatively good auxiliary effects on hypertension and hyperlipidemia.

The present invention relates to pyrimidinones and pyridones and derivatives thereof, and pharmaceutically acceptable salts thereof. Also included is a method of treatment of inflammation, rheumatoid arthritis, Pagets disease, osteoporosis, multiple myeloma, uveititis, acute or chronic myelogenous leukemia, pancreatic β cell destruction, osteoarthritis, rheumatoid spondylitis, gouty arthritis, inflammatory bowel disease, adult respiratory distress syndrome (ARDS), psoriasis, Crohn's disease, allergic rhinitis, ulcerative colitis, anaphylaxis, contact dermatitis, asthma, muscle degeneration, cachexia, Reiter's syndrome, type I diabetes, type II diabetes, bone resorption diseases, graft vs. host reaction, Alzheimer's disease, stroke, myocardial infarction, ischemia reperfusion injury, atherosclerosis, brain trauma, multiple sclerosis, cerebral malaria, sepsis, septic shock, toxic shock syndrome, fever, myalgias due to HIV-1, HIV-2, HIV-3, cytomegalovirus (CMV), influenza, adenovirus, the herpes viruses or herpes zoster infection in a mammal comprising administering an effective amount a compound as described above.

The high-efficiency production process of Dahongpao tea type high-end handmade Zihongpao tea, the process steps include: (1) withering, (2) making green, (3) frying, (4) rolling, (5) drying, (6) Refining, its technical features are: the method of withering in the withering process of (1) is: performing sunlight withering on the purple red robe tea green, or evenly spreading the tea green on the withering groove to heat and wither; the (2) The greening process is completed on the withering tank, and the fermentation and standing process is carried out according to the conventional technology of Dahongpao tea, which achieves partial cell destruction of the green leaves of Zihongpao tea, thereby promoting enzymatic oxidation. Lay it on the withering groove, and in the process of making green, manually hold the needle board and press the purple red robe tea green on the withering groove horizontally to form evenly distributed tiny needle holes on the purple red robe tea green leaf, which destroys some cells of the purple red robe tea green leaf. Thereby, the enzymatic oxidation is promoted; the structure of the acupuncture plate is as follows: stainless steel needles with sharp heads are arranged in an orderly manner on the support plate, and a handle is installed on the back of the support plate to form the acupuncture plate.