Arthrobacter strain ZJ-H and application thereof

A technology of ZJ-H and Arthrobacter, applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems of salt-alkali tolerance and oil degradation efficiency, and achieve good emulsification The effect of activity, good emulsification and good degradability

Active Publication Date: 2019-12-10
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0008] The above-mentioned existing technologies also have the problem of difficulty in balancing salt-alkali tolerance and oil degradation efficiency

Method used

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  • Arthrobacter strain ZJ-H and application thereof
  • Arthrobacter strain ZJ-H and application thereof
  • Arthrobacter strain ZJ-H and application thereof

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Embodiment Construction

[0030] The technical solution of the present invention will be further described below in conjunction with specific embodiments. The raw materials used in the present invention are conventional commercial products unless otherwise specified; the methods used in the present invention, unless otherwise specified, are conventional methods in the field.

[0031] 1. Screening of strain ZJ-H

[0032] (1) Sampling: The oil-based drill cuttings sample comes from the Bohai Oilfield and is taken from CNOOC Bihai Environmental Technical Service Company. The water content is 15%, the pH is 7.9, and the salinity is 1.5%. The crude oil is obtained from the oil extraction plant of Bohai Oilfield. Through previous sampling and screening studies, the inventor found that strains isolated and screened from oil-based drill cuttings tend to have stronger crude oil degradation ability than strains screened from oily wastewater or oil-contaminated soil.

[0033] (2) Enrichment and acclimatization: Weigh ...

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Abstract

The invention provides an arthrobacter strain ZJ-H and an application of the arthrobacter strain ZJ-H to the degradation of petroleum hydrocarbons in a high-saline-alkali environment. The strain ZJ-His selected from oil-based drilling cutting samples of the Bohai sea oilfield, which are provided by a China sea petroleum Bihai environmental protection technology service company. The strain ZJ-H isstrong in tolerance to the high-saline-alkali environment and can normally survive in the environment with the salinity of 1-5% and the pH value of 7-11; the strain ZJ-H has relatively high petroleumdegradation rate in the saline-alkali environment, which reaches 74.45%; a metabolic product of the strain ZJ-H has relatively high emulsifying activity, has a good emulsifying effect on petroleum ether, and is beneficial to further decomposition and utilization of the petroleum ether by the strain; and the strain ZJ-H has a good degradation capability for n-alkanes (including long-chain alkanesof C20-C30) in raw petroleum, the degradation rate exceeds 83%, and especially, the degradation rate of C25-C30 reaches about 95%.

Description

Technical field [0001] The invention relates to the field of functional microorganisms, in particular to an Arthrobacter strain ZJ-H and its application in degrading petroleum hydrocarbons in a high-saline environment. Background technique [0002] With the development of the petroleum industry, oil and gas exploration and exploitation activities have become more frequent. Oil-based drill cuttings continuously produced in the process of oil and gas production and development enter the soil environment and groundwater, posing a serious threat to human health. The penetration of oil into the soil will contaminate the soil layer, resulting in reduced water permeability and water permeability, and further aggravation of compaction and salinization. In addition, oil-based drill cuttings have the characteristics of high salt content and serious organic pollution. Therefore, many oilfield soils The environment is often polluted by high salinity and petroleum hydrocarbons. [0003] Microb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C02F101/32C12R1/06
CPCB09C1/10C02F3/34C12N1/205C12R2001/06
Inventor 刘宪斌刘佳博赵兴贵林颖青
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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