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52results about How to "High emulsifying activity" patented technology

Preparation method of emulsified stable type soy isolate protein

The invention relates to a preparation method of emulsified stable type soy isolate protein and belongs to the field of process technology of soy protein. The preparation method comprises the main steps as follows: preparing a soy isolate protein solution with the ratio of deionized water to soy isolate protein used as a raw material being (5-25):1 w / w; fully hydrating, and then stirring and preheating at 40-60 DEG C; regulating pH to be 6.5-7.5; adding papain for enzymolysis to obtain a sample of different hydrolysis degrees; then killing enzyme for 5 min at 90 DEG C; cooling to room temperature; regulating pH to be neutral; centrifuging; taking supernate to carry out freeze drying or spray drying; preparing a sample solution with the ratio of deionized water to the sample of different hydrolysis degrees being (5-25):1 w / w; preheating at 30-50 DEG C; regulating pH to be 7.0; adding transglutaminase to start crosslinking; after 1h, heating for 5 min at 80 DEG C to inactivate the enzyme; cooling to room temperature; then regulating pH to be neutral; centrifuging; taking supernate; and performing freeze drying or spray drying to obtain the highly emulsified stable type soy isolate protein. The preparation method for the emulsified stable type soy isolate protein is economic and effective; and the emulsion stability of the soy protein is remarkably improved.
Owner:JIANGNAN UNIV

Active thermophilic bacteria mutant strain and application thereof in oil displacement

The invention aims to provide an active thermophilic bacteria mutant strain and an application thereof in oil displacement. The invention provides an XS2-450 (Geobacillus sp.) which is obtained through normal-pressure room-temperature plasma mutagenesis to an original strain Geobacillus sp.XS2, wherein the Geobacillus sp.XS2 is assigned the accession number CGMCC No.9430. By means of the normal-pressure room-temperature plasma mutagenesis method to obtain the Geobacillus sp. XS2-450, emulsification activity of an emulsifying agent can be greatly increased and the emulsification activity on methylbenzene and dimethylbenzene can reach 100%. Emulsification stability is good. 70%-100% of the emulsification activity can be maintained after 30-day standing. The emulsification activity is not obviously reduced after serial passages of a strain being carried out for 20 times. The mutant strain Geobacillus sp. XS2-450 is subjected to industrially amplifying production in the invention and is applied in a pilot-scale experiment of a single well in an oil field. Oil production amount in Liu 55-4 oil well in Changqing oil field is increased by 12 times and water content in a produced liquid is reduced by more than 70%. The mutant strain has a great economic value and a potential in large-scale application.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI

Method for improving emulsifying property of isolated soybean protein under acidic condition, and products of isolated soybean protein

The invention discloses a method for improving the emulsifying property of isolated soybean protein under the acidic condition, and products of the isolated soybean protein, belonging to the field of modification of the isolated soybean protein. The method comprises the steps of (1) carrying out pretreatment on the isolated soybean protein by extrusion; (2) drying and crushing the product obtained by the treatment in the step (1), and then dissolving the product with water to obtain isolated soybean protein water solution; (3) feeding protease into the isolated soybean protein water solution, and carrying out an enzymolysis reaction; (4) carrying out enzyme deactivation, cooling, concentrating and drying to obtain the modified isolated soybean protein. The modified isolated soybean protein obtained by the modification method has the emulsifying property of 0.49m<2> / g when the pH value is 4, so that the emulsifying property of the modified isolated soybean protein is improved by 172% compared with the common isolated soybean protein; the emulsion stability of the modified isolated soybean protein is 16.2 minutes and is improved by 20% compared with the common isolated soybean protein; the modified isolated soybean protein prepared by the method can be applied to preparing dairy products, drink products, flour products, meat products, or the like.
Owner:SOYBEAN TECH DEV RES CENT HEILONGJIANG PROV

Method for reducing allergenicity of special soybean isolation protein of soy-based infant formula powder

ActiveCN102488075AAllergenicity reductionUnaffected or minimally affected by natural flavorProtein foodstuffs working-upWater bathsProtein solution
The invention discloses a method for reducing allergenicity of special soybean isolation protein of soy-based infant formula powder, belonging to the field of food safety. Soybean isolation protein prepared from non-transgenic and non-radiation soybean is used as the raw material; the special soybean isolation protein is subjected to compound modification by adopting an ultra-high static pressurenon-thermal physical method cooperatively with a compound enzyme method, therefore, the allergenicity of the special soybean isolation protein is obviously reduced; meanwhile, functionalities, such as an nitrogen soluble index, emulsifying activity, viscosity, thixotropy, in-vitro digestibility coefficient and the like, and nutritional quality are improved. Detailed parameters of the ultra-high static pressure and compound enzyme enzymatic treatment are as follows: weighing a certain amount of the soybean isolation protein, mixing and modulating the soybean isolation protein into 1-1.65% of uniform slurry, then, transferring the slurry into an aluminium foil bag, sealing, and vaccumizing; pre-heating an ultra-high static pressure water bath circulation device to 20-25 DEG C, then, puttingthe soybean isolation protein in an ultra-high static pressure processing cavity, increasing pressure to 315-385 MPa with the pressure increasing rate of 250 MPa/min, and keeping the pressure for 15-20 min with the pressure relieving rate of 300 MPa/min; and concentrating a soybean isolation protein solution in vacuum to 5-15%, then, transferring the soybean isolation protein solution into an enzymatic tank, adjusting the temperature of enzymatic liquid to 48-55 DEG C, adjusting a pH value of the enzymatic liquid to 6-7.5, wherein the use amount of special compound enzyme (preparing neutral protease and flavourzyme according to the mass ratio of 1:1 in a mixed and compound manner) is 1.5-2.5%; and the enzymatic time is 1.2-2.5 h; and then, obtaining a finished product by enzyme inactivating the enzymatic liquid, desalting, concentrating, atomizing and drying. When the product obtained by using the method disclosed by the invention is detected by ELISA (Enzyme-Linked Immuno Sorbent Assay), the allergen content is reduced by 87.49-90.75%.
Owner:兆隆康源(山西)生物科技有限公司

Stable microemulsion based on modified perilla seed protein and preparation method of microemulsion

The invention discloses a stable microemulsion based on modified perilla seed protein and a preparation method of the microemulsion, and belongs to the technical field of protein modification. The method comprises the following steps: adjusting the moisture of perilla seed meal, performing extrusion pretreatment, then performing crushing and sieving, adding water, performing mixing to prepare a perilla seed meal mixed solution, adjusting the pH value and temperature, adding protease, carrying out enzymolysis, performing cooling to room temperature, adjusting the pH value to neutrality, carrying out ultrafiltration treatment to obtain perilla seed protein with the molecular weight cutoff of 30-100kDa, and performing concentrating and drying to obtain the perilla seed protein with emulsifying activity; and uniformly mixing perilla seed protein, sucrose ester, flaxseed gum and polyglycerol ester, then adding flaxseed oil and a preservative, and finally, performing high-speed shearing emulsification and high-pressure homogenization to obtain the stable microemulsion. The preparation method is simple in production process, small in required enzyme dosage and low in production cost, andthe prepared microemulsion is good in stability.
Owner:SHAANXI UNIV OF SCI & TECH

Preparation method of modified phosphatide-soybean protein composite emulsion

The invention relates to a preparation method of modified phosphatide-soybean protein composite emulsion, and belongs to the field of fine and deep processing of soybean protein. The method comprises the following steps: (1) dissolving soybean lecithin in distilled water so as to obtain a phosphatide solution, preheating the phosphatide solution, performing pulsed electric field treatment on the preheated phosphatide solution, carrying out vacuum freeze-drying, and dissolving the obtained material in a phosphoric acid buffer solution so as to obtain a modified phosphatide solution; (2) dissolving soy protein isolate in the phosphoric acid buffer solution so as to obtain a soybean protein solution; and (3) mixing and stirring the modified phosphatide solution and the soybean protein solution so as to obtain mixed liquor, adding olive oil to the mixed liquor, and performing homogenizing treatment on the mixed liquor and the olive oil so as to obtain the composite emulsion. The method is simple in technology and safe to operate; the soybean lecithin is modified through a pulsed electric field, and then the modified soybean lecithin and the soybean protein are composited, so that the prepared composite emulsion is high in emulsifying activity, high in emulsifying stability, and safe and harmless, and has better application prospects.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
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