Arthrobacter strain zj-h and its application

A technology of ZJ-H and Arthrobacter, which is applied in the direction of bacteria, biological water/sewage treatment, microorganisms, etc., can solve the problems of saline-alkali tolerance and oil degradation efficiency, and achieve good emulsifying activity, good emulsifying effect, The effect of good degradability

Active Publication Date: 2021-11-23
TIANJIN UNIV OF SCI & TECH
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Problems solved by technology

[0008] The above-mentioned existing technologies also have the problem of difficulty in balancing salt-alkali tolerance and oil degradation efficiency

Method used

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  • Arthrobacter strain zj-h and its application
  • Arthrobacter strain zj-h and its application
  • Arthrobacter strain zj-h and its application

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Embodiment Construction

[0030] The technical solutions of the present invention will be further described below in conjunction with specific embodiments. The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0031] 1. Screening of strain ZJ-H

[0032] (1) Sampling: The oil-based drilling cuttings samples are from Bohai Oilfield, taken from CNOOC Bihai Environmental Protection Technology Service Company, with a moisture content of 15%, a pH of 7.9, and a salinity of 1.5%. Crude oil is obtained from Bohai Oilfield Oil Production Plant. The inventors have found through previous sampling and screening studies that strains isolated and screened from oil-based drilling cuttings tend to have a stronger ability to degrade crude oil than strains screened from oily wastewater or oil-contaminated soil.

[0033] (2) Enrichment and...

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Abstract

The invention provides an Arthrobacter strain ZJ-H and its application in degrading petroleum hydrocarbons in a high-saline environment. Strain ZJ‑H was screened from oil-based drilling cuttings samples from Bohai Oilfield, provided by CNOOC Bihai Environmental Technology Service Company. Strain ZJ‑H has a strong tolerance to high saline-alkali environment, and can survive normally in an environment with a salinity of 1-5% and a pH of 7-11; strain ZJ-H has a high degradation rate of petroleum in a saline-alkali environment , reaching 74.45%; the metabolites of bacterial strain ZJ‑H have better emulsifying activity, and have good emulsifying effect on petroleum ether, which is beneficial to the further decomposition and utilization of bacterial strain to petroleum ether; Long-chain alkanes including C20-C30) have good degradation ability, the degradation rate exceeds 83%, especially the degradation rate of C25-C30 reaches about 95%.

Description

technical field [0001] The invention relates to the field of functional microorganisms, in particular to an Arthrobacter strain ZJ-H and its application in degrading petroleum hydrocarbons in a high-saline environment. Background technique [0002] With the development of the petroleum industry, oil and gas exploration and exploitation activities have become more and more frequent. During the process of oil and gas production and development, oil-based drilling cuttings are continuously produced into the soil environment and groundwater, posing a serious threat to human health. Oil penetration into the soil will pollute the soil layer, resulting in a decrease in water permeability and water permeability, and further aggravation of compaction and salinization. In addition, oil-based drilling cuttings have the characteristics of high salt content and serious organic pollution. Therefore, many oilfield soils The environment is often double polluted by high salinity and petroleu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C02F101/32C12R1/06
CPCB09C1/10C02F3/34C12N1/205C12R2001/06
Inventor 刘宪斌刘佳博赵兴贵林颖青
Owner TIANJIN UNIV OF SCI & TECH
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