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Active thermophilic bacteria mutant strain and application thereof in oil displacement

A composite oil displacement agent and bacterial strain technology, applied in the direction of mutant preparation, bacteria, drilling composition, etc., can solve the problems of not being able to significantly reduce surface tension, narrow growth temperature range, poor environmental adaptability, etc., and achieve major economic benefits Value and large-scale application, improved emulsification activity, and good emulsification stability

Active Publication Date: 2014-11-19
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the strains that metabolize and synthesize biological emulsifiers have the following problems in application: 1) the activity is unstable, and the activity decreases seriously in actual production; the activity is high in a short period of time, and the activity decreases for a long time; The temperature range is narrow, and the actual environmental temperature changes greatly, so that the strain cannot grow rapidly and exhibit activity; 3) The bioemulsifier has strong emulsifying activity, but cannot significantly reduce the surface tension, resulting in slow oil displacement activity when used alone

Method used

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  • Active thermophilic bacteria mutant strain and application thereof in oil displacement
  • Active thermophilic bacteria mutant strain and application thereof in oil displacement
  • Active thermophilic bacteria mutant strain and application thereof in oil displacement

Examples

Experimental program
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Effect test

Embodiment 1

[0017] This example illustrates a method for plasma mutagenesis of an original strain.

[0018] The culture medium formulation (w / v) used wherein:

[0019] Solid plate LB (Luria-Bertani) medium: sodium chloride 10%, tryptone 10%, yeast extract 5%, agar 2%, pH 7.5.

[0020] Liquid LB medium: 10% sodium chloride, 10% tryptone, 5% yeast extract, pH 7.5. The specific method for carrying out plasma mutagenesis with the original strain Geobacillus pallidusXS2 is as follows:

[0021] 1. Prepare the original strain Geobacillus pallidusXS2 bacterial suspension

[0022] The original strain Geobacillus pallidusXS2 is a strain of thermophilic Geobacillus isolated and screened from the Yaerxia oil reservoir in Yumen Oilfield, Gansu, China by our laboratory; it is a strain of Geobacillus pallidus (Geobacillus pallidus), and the preservation number of this strain is CGMCC No.8338, was deposited on October 15, 2013 at the General Microbiology Center of the Chinese Microbiological Culture C...

Embodiment 2

[0029] This example illustrates the screening method for producing highly active bioemulsifier strains.

[0030] The culture medium formulation (w / v) used wherein:

[0031] Solid plate LB (Luria-Bertani) medium: sodium chloride 10%, tryptone 10%, yeast extract 5%, agar 2%, pH 7.5.

[0032] Liquid LB medium: 10% sodium chloride, 10% tryptone, 5% yeast extract, pH 7.5.

[0033] Fermentation medium: glucose 2%, sodium nitrate 0.3%, potassium dihydrogen phosphate 0.042%, dipotassium hydrogen phosphate 0.12%, ferrous sulfate 0.005%, magnesium sulfate 0.05%, calcium chloride 0.005%, vitamin 0.02%. Trace elements 0.1%, pH 7.5.

[0034] Trace elements: boric acid 0.025%, copper sulfate 0.05%, manganese sulfate 0.05%, sodium molybdate 0.006%, zinc sulfate 0.07%.

[0035] Vitamins: Calcium D-pantothenate 0.004%, inositol 0.002%, niacin 0.004%, vitamin 6.004%, p-aminobenzoic acid 0.002%, vitamin B12 0.05%.

[0036] The screening steps are as follows:

[0037] 1. Primary screening of...

Embodiment 3

[0044] In this example, the emulsifying activity of the mutant strain XS2-450 on various hydrocarbons was determined.

[0045] The method for measuring emulsifying activity provided by the present invention is to mix the mutant strain fermentation stoste with seven different hydrocarbons (toluene, xylene, n-hexadecane, liquid paraffin, soybean oil, diesel oil, olive oil) in a certain ratio, vortex Rotate and oscillate for 2 minutes, let stand for 24 hours, emulsifying activity = height of emulsified layer / total height of liquid level * 100%. The experimental results show that the mutant strain XS2-450 has higher emulsifying activity for the above seven hydrocarbons after system optimization. Wherein the toluene can reach 100% at the highest, which is 29.6% higher than the activity of the original bacterial strain.

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Abstract

The invention aims to provide an active thermophilic bacteria mutant strain and an application thereof in oil displacement. The invention provides an XS2-450 (Geobacillus sp.) which is obtained through normal-pressure room-temperature plasma mutagenesis to an original strain Geobacillus sp.XS2, wherein the Geobacillus sp.XS2 is assigned the accession number CGMCC No.9430. By means of the normal-pressure room-temperature plasma mutagenesis method to obtain the Geobacillus sp. XS2-450, emulsification activity of an emulsifying agent can be greatly increased and the emulsification activity on methylbenzene and dimethylbenzene can reach 100%. Emulsification stability is good. 70%-100% of the emulsification activity can be maintained after 30-day standing. The emulsification activity is not obviously reduced after serial passages of a strain being carried out for 20 times. The mutant strain Geobacillus sp. XS2-450 is subjected to industrially amplifying production in the invention and is applied in a pilot-scale experiment of a single well in an oil field. Oil production amount in Liu 55-4 oil well in Changqing oil field is increased by 12 times and water content in a produced liquid is reduced by more than 70%. The mutant strain has a great economic value and a potential in large-scale application.

Description

technical field [0001] The invention belongs to the technical field of screening and application of active microbial strains, and in particular relates to a mutant strain of thermophilic microorganisms and its application. Background technique [0002] Surfactant is a general term for a class of amphiphilic molecules, which contain both hydrophilic and lipophilic groups, and can significantly reduce the surface tension of solvents or liquid-liquid interfacial tension or increase the stability of emulsified systems at very low concentrations. Surfactants have various activities, such as emulsification, surface adsorption, solubility, wettability, foaming, decontamination, etc., and are widely used in leather, cosmetics, construction, petroleum industry, environmental engineering and other fields. [0003] Surfactants produced by living organisms are called biosurfactants. Compared with chemical surfactants, biosurfactants have the advantages of less dosage, good selectivity,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/01C09K8/582C12R1/10
Inventor 黄志勇董大媛王兴彪蔡明昱
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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