Active thermophilic bacteria mutant strain and application thereof in oil displacement
A composite oil displacement agent and bacterial strain technology, applied in the direction of mutant preparation, bacteria, drilling composition, etc., can solve the problems of not being able to significantly reduce surface tension, narrow growth temperature range, poor environmental adaptability, etc., and achieve major economic benefits Value and large-scale application, improved emulsification activity, and good emulsification stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0016] Example 1
[0017] This example illustrates the method of plasma mutagenesis of the original strain.
[0018] The medium formula used (w / v):
[0019] Solid plate LB (Luria-Bertani) medium: sodium chloride 10%, tryptone 10%, yeast extract 5%, agar 2%, pH 7.5.
[0020] Liquid LB medium: sodium chloride 10%, tryptone 10%, yeast extract 5%, pH 7.5. The specific method for plasma mutagenesis with the original strain Geobacillus pallidusXS2 is as follows:
[0021] 1. Prepare the original strain Geobacillus pallidusXS2 suspension
[0022] The original strain Geobacillus pallidus XS2 is a strain of Geobacillus thermophilus isolated and screened by our laboratory from the Yaerxia oil reservoir in Yumen Oilfield, Gansu, China; it is a Geobacillus pallidus strain (Geobacillus pallidus), the deposit number of this strain is CGMCC No. 8338, deposited on October 15, 2013, is located at the General Microbiology Center of the Chinese Academy of Sciences Institute of Microbiology, No. 3 Beichen ...
Example Embodiment
[0028] Example 2
[0029] This example illustrates the screening method of strains producing high-activity bioemulsifiers.
[0030] The medium formula used (w / v):
[0031] Solid plate LB (Luria-Bertani) medium: sodium chloride 10%, tryptone 10%, yeast extract 5%, agar 2%, pH 7.5.
[0032] Liquid LB medium: sodium chloride 10%, tryptone 10%, yeast extract 5%, pH 7.5.
[0033] Fermentation medium: glucose 2%, sodium nitrate 0.3%, potassium dihydrogen phosphate 0.042%, dipotassium hydrogen phosphate 0.12%, ferrous sulfate 0.005%, magnesium sulfate 0.05%, calcium chloride 0.005%, vitamin 0.02%. The trace element is 0.1%, and the pH is 7.5.
[0034] Trace elements: 0.025% of boric acid, 0.05% of copper sulfate, 0.05% of manganese sulfate, 0.006% of sodium molybdate, 0.07% of zinc sulfate.
[0035] Vitamins: D-calcium pantothenate 0.004%, inositol 0.002%, niacin 0.004%, vitamin 6.004%, p-aminobenzoic acid 0.002%, vitamin B12 0.05%.
[0036] The screening steps are as follows:
[0037] 1. Primary...
Example Embodiment
[0043] Example 3
[0044] In this example, the emulsifying activity of the mutant strain XS2-450 on various hydrocarbons was determined.
[0045] The emulsification activity determination method provided by the present invention is to mix the fermentation stock solution of the mutant strain with seven different hydrocarbons (toluene, xylene, n-hexadecane, liquid paraffin, soybean oil, diesel oil, olive oil) in a certain proportion, and then vortex Rotate and shake for 2 minutes, and let stand for 24 hours, emulsification activity=emulsified layer height / total height of liquid surface*100%. The experimental results show that the mutant strain XS2-450 has high emulsifying activity on the above seven hydrocarbons after system optimization. Among them, toluene is up to 100%, which is 29.6% higher than the original strain.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap