The invention discloses Acinetobacter piterii and its application, wherein, the Acinetobacter piteter was preserved in the General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC) on November 16, 2015, and the address of the preservation unit is Chaoyang, Beijing Institute of Microbiology, Chinese Academy of Sciences, No. 1, Yard 1, Beichen West Road, District, the preservation number is CGMCC NO.11670, and the preservation name is Acinetobacter pittii SYJ1‑3. The Acinetobacter piterii of the present invention can grow and proliferate with petroleum as a carbon source, utilize its own metabolism to degrade petroleum, and can be effectively used in the treatment of petroleum wastewater, petroleum-contaminated soil, etc., not only has high petroleum degradation efficiency, but also is simple and convenient to operate and low in cost. lower.