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Application of Neosphingobacter claff in the preparation of microbial agent for removing cod in petroleum wastewater

A new sphingobacterium, microbial agent technology, applied in the field of microorganisms, can solve the problems of high cost, plant death, high oxygen consumption, etc., achieve stable production process, broad application prospects, and solve the effect of processing problems

Active Publication Date: 2021-10-15
QINGDAO GENYUAN BIOLOGICAL TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The illegal discharge of petroleum wastewater will cause great harm: (1) The decomposition process of petroleum wastewater will consume a large amount of oxygen, resulting in a decrease in the concentration of dissolved oxygen in the water body, resulting in the death of aquatic animals and plants due to hypoxia, resulting in the destruction of ecological balance; Wastewater pollutes the soil, which will cause soil pollution, cause plant death, and cause huge losses to the ecosystem and agriculture and animal husbandry; (3) Aromatic hydrocarbons in petroleum wastewater are difficult to be biodegraded, which will lead to their continuous accumulation through the food chain, and eventually produce harmful substances. Distortion, carcinogenic and other consequences that seriously endanger human life and health
At present, the difficulty in treating petroleum wastewater is mainly due to the large amount of petroleum wastewater and the difficulty of treatment. At present, the main method is physical method, which removes pollutants in petroleum wastewater through adsorption, extraction and other methods, but these methods are less effective and costly. And it can only transfer pollutants, but cannot achieve the purpose of removal. Therefore, there is still no better method for petroleum wastewater treatment

Method used

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  • Application of Neosphingobacter claff in the preparation of microbial agent for removing cod in petroleum wastewater
  • Application of Neosphingobacter claff in the preparation of microbial agent for removing cod in petroleum wastewater
  • Application of Neosphingobacter claff in the preparation of microbial agent for removing cod in petroleum wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Screening, isolation and identification of Neosphingobacter claffi GBW-HB1906

[0033] 1. Screening and purification of Neosphingobacter claffi GBW-HB1906

[0034] Take 10ml of mixed wastewater from each section of the biochemical system of the sewage plant, add it to 200mL of PBS buffer solution, shake for 20min to fully mix the sample, centrifuge at 800rpm for 1min, collect the supernatant of the sample, and set aside. Take 10mL of the supernatant of the above samples and add them to Erlenmeyer flasks containing 200mL salt-tolerant growth microorganism enrichment and screening liquid medium, culture at 30°C, 180rpm constant temperature shaker for 3 days, and enrich for 3 times. After gradient dilution of the cultured bacterial suspension, 100 μL was applied to the solid medium for isolation and purification of salt-tolerant growth microorganisms, and placed in a 30°C incubator for cultivation. After 48 hours, single colonies of different shapes were picked...

Embodiment 2

[0041] Embodiment 2: the growth curve of Neosphingobacterium clavus GBW-HB1906 in petroleum wastewater

[0042] Take 100ml of wastewater from a petrochemical enterprise, insert Neosphingobacter claffi GBW-HB1906 into the petroleum wastewater with an inoculation amount of 1‰, and cultivate it in a constant temperature shaking shaker at 30°C, and take samples every 12h for colony counting, continuous Sample 3d. Colony counting method: Take 1ml of the sample to be tested, put it into a sterilized test tube containing 9ml of sterile water, dilute it to an appropriate concentration in turn, take 100 μL of the diluted solution in NA solid medium, and use a coating rod to coat it. Place the coated plate in a constant temperature incubator at 30°C for 24 hours and take it out for counting. Test results such as image 3 As shown, after the New Sphingobacterium clavus GBW-HB1906 was cultivated in petroleum wastewater for 3 days, the amount of bacteria could reach 2×10 9 cfu / mL, indic...

Embodiment 3

[0043] Embodiment 3: COD changes of petroleum wastewater treated by Neosphingobacterium clavus GBW-HB1906

[0044] Take 100ml of wastewater from a petrochemical enterprise (COD is 302.7mg / L), insert Neosphingobacter claffi GBW-HB1906 into the petroleum wastewater with an inoculation amount of 1‰, and cultivate it in a constant temperature shaking shaker at 30°C , Sampling every 12 hours to measure the COD value of the water sample. The COD determination method is determined by the HJ 828-2017 Determination of Chemical Oxygen Demand in Water Quality-Potassium Dichromate Method. Test results such as Figure 4 As shown, after being treated by the Neosphingobacterium clavus GBW-HB1906 for 3 days, the COD of the petroleum wastewater was reduced to 87.52%.

[0045] In the present invention, after neosphingobacterium claffi GBW-HB1906 can also be prepared into fermentation broth, the fermentation broth can be directly or mixed with other carriers to remove COD in petroleum wastewate...

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Abstract

The invention provides the application of the neosphingobacterium clavus in the preparation of a microbial bacterial agent for removing COD in petroleum wastewater. The neosphingobacter clavferi is the neosphingobacter clavferi GBW‑HB1906 with a preservation number of CGMCC No.20365. This bacterium has tolerance to euryotic and can grow normally at a salt concentration of 15‑35‰. And it has the ability to tolerate and decompose alkanes and degrade COD of petroleum wastewater. The microbial agent prepared by using Neosphingobacterium clavus GBW‑HB1906 has stable properties and is easy to use. It can effectively remove COD in petroleum wastewater, quickly and efficiently solve the problem of petroleum wastewater treatment, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to the application of Neosphingobacter clavferi in the preparation of microbial bacterial agents for removing COD in petroleum wastewater. Background technique [0002] Petroleum wastewater refers to the wastewater generated in the process of petroleum exploration and refining. With the continuous advancement of industrialization, the volume of petroleum wastewater is increasing, and the treatment of petroleum wastewater has received more and more attention. [0003] The main components of petroleum wastewater are saturated alkanes, unsaturated alkanes, cycloalkanes and aromatic hydrocarbons, as well as sulfur, phenol, ammonia nitrogen, cyanide, etc. The composition is extremely complex, so it is extremely difficult to treat. The illegal discharge of petroleum wastewater will cause great harm: (1) The decomposition process of petroleum wastewater will consume a large am...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F103/10C02F103/36C12R1/01
CPCC02F3/34C02F2103/10C02F2103/365C12N1/20
Inventor 王熙涛袁绍辉张晓春赵金超赵莉
Owner QINGDAO GENYUAN BIOLOGICAL TECH GRP
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