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Acinetobacter pittii and application thereof

A technology of bacilli and microbial strains, applied in the field of microbiology, can solve problems such as frequent inoculation, poor adaptability of the bacterial flora, and difficulty in reaching the reinjection water quality standard for residual oil content

Active Publication Date: 2016-06-08
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing petroleum wastewater biological treatment technology generally has problems such as poor microbial oil degradation ability, poor adaptability of bacterial flora, frequent inoculation, etc., resulting in high operating costs, difficulty in continuous operation, and difficulty in reinjection of residual oil content after treatment. Water quality standards make it difficult to realize large-scale industrial application of biological treatment technology
[0005] Therefore, the current technology related to the biological treatment of petroleum wastewater still needs to be improved.

Method used

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  • Acinetobacter pittii and application thereof
  • Acinetobacter pittii and application thereof
  • Acinetobacter pittii and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Screening of Acinetobacterpittii SYJ1-3 with petroleum degradation ability

[0031] Screening steps: Take 500g soil samples from 10 long-term oil-contaminated soils in the Changqing Oilfield site 5-10cm below the ground surface. After mixing them, take 10g of the mixed soil samples and add them to 250mL shaking with 100mL LB medium. Microbial enrichment in the bottle, specifically, culture at 28-30°C and 180rpm on a shaker for 5 days to obtain an enriched solution. 1mL of the enriched solution obtained above is absorbed and transferred to fresh LB medium to continue the microorganism For enrichment, after culturing for 5 days, absorb 1mL of the enriched solution obtained and transfer to an inorganic salt medium with petroleum as the sole carbon source (formulation: NH 4 NO 3 (2g / L), Na 2 HPO 4 ·12H 2 O(3.8g / L), KH 2 PO 4 (1.5g / L), NaCl(1g / L), MgSO 4 (0.2g / L), FeSO 4 ·7H 2 O0.05g / L, anhydrous CaCl 2 0.05g / L, crude oil (5g / L), pH7.0~7.2, and sterilization at 121℃ f...

Embodiment 2

[0035] Example 2: Laboratory shake flask experiment to determine the effect of AcinetobacterpittiiSYJ1-3 in degrading petroleum

[0036] A loop of colonies was selected from the Acinetobacterpittii SYJ1-3 purification plate obtained in Example 1 and connected to LB medium. The volume of the shake flask was 30% of the volume of the shake flask, the culture temperature was 28-30°C, and the culture speed was 150-200rpm / min , The cultivation time is 10-16h, and SYJ1-3 seed culture solution is obtained. Weigh 100mg of petroleum into a shaker flask containing 90ml of inorganic salt medium, and then inoculate 10ml of the SYJ1-3 seed culture solution obtained above. At the same time, use the petroleum-containing mineral salt medium without SYJ1-3 seed culture as a control The initial petroleum concentration in the solution of the experimental group and the control group was 1 mg / mL. Put the above-mentioned experimental group (containing SYJ1-3 seed culture solution) and control group (w...

Embodiment 3

[0038] Example 3: Application of AcinetobacterpittiiSYJ1-3 in a small-scale SBR device and environmental adaptability test

[0039] The inlet water used in this experiment is artificially prepared simulated wastewater and raw water produced from Changqing Oilfield. The artificially prepared simulated wastewater formula is mainly composed of NH 4 Cl(0.02g / L), K 2 HPO 4 , NaCl(2g / L), NaHCO 3 (4g / L), Na 2 CO 3 (1g / L), MgSO 4 (0.2g / L), Fe 2 SO 4 ·7H 2 O(0.05g / L), anhydrous CaCl 2 (0.05g / L), glucose (0.1g), petroleum (50~500mg / L), pH (7.5~8.5). The sludge inoculated in the SBR when the reactor is started is taken from the activated sludge of a sewage treatment plant. The sludge inoculation amount is 1530mg / L. When the SBR process runs to the 15th day, the sludge concentration is reduced to 390mg / L, and the amplified AcinetobacterpittiiSYJ1-3 strain is added to the SBR process at a rate of about 10% (volume percentage). The SBR process operated continuously for 82 days. During the ope...

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Abstract

The invention discloses Acinetobacter pittii and application thereof. The Acinetobacter pittii is collected at the China General Microbiological Culture Collection Center (CGMCC) on 16th, Nov. 2015 in the Institute of Microbiology of Chinese Academy of Sciences at Number 3, Yard 1 in Beichen West Road of Chaoyang District in Beijing, numbered as CGMCC No.11670 and named as Acinetobacter pittii SYJ1-3. The Acinetobacter pittii can grow and proliferate by taking petroleum as a carbon source, can degrade petroleum by utilizing own metabolism, can be effectively used for treatment of petroleum wastewater and petroleum polluted soil and is high in petroleum degrading efficiency, simple and convenient to operate and low in cost.

Description

Technical field [0001] The present invention relates to the field of microorganisms, specifically, to Acinetobacter peterii and its uses, and more specifically, to Acinetobacter peter's and its use in degrading petroleum, and a method for degrading petroleum. Background technique [0002] With the continuous increase in energy demand for economic development, most of the oilfields in my country have entered the second or third oil recovery period. The increase in the difficulty of extraction has led to the improvement of oil recovery through water injection or changing the characteristics of injected water. Mining method. Every year, a large amount of clean water with good compatibility with the underground reservoir is injected into the target layer, and a large amount of oily wastewater is extracted from the target layer with the produced fluid. The comprehensive water cut of the produced fluid of most of the onshore oil fields in my country reaches 80% As mentioned above, the t...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/32
CPCB09C1/10C02F3/344C02F2101/32C12N1/205C12R2001/01
Inventor 左剑恶李晓霞
Owner TSINGHUA UNIV
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