Preparation method of low-methoxy hawthorn pectin

A technology of low methoxyl group and hawthorn fruit, applied in the field of food deep processing, can solve the problem of not clearly and accurately stating the characteristics of pectin products in the preparation technology of pectin products, and achieves significant social and economic benefits, high purity, and good emulsification. The effect of stability

Active Publication Date: 2021-01-12
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Zhu Meiyun et al. (1993, Henan Science and Technology, 14~15) researched that LMP can be extracted from hawthorn, but the article did not clearly and accurately state the preparation technology of the obtained pectin product and the corresponding characteristics of the pectin product

Method used

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  • Preparation method of low-methoxy hawthorn pectin
  • Preparation method of low-methoxy hawthorn pectin

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Experimental program
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Effect test

Embodiment 1

[0030] The present embodiment chooses Dajinxing (DA), the main product of Shandong area hawthorn variety, as the test raw material, and the specific implementation steps are as follows:

[0031] 1. Preparation of hawthorn powder

[0032] Fresh hawthorn fruits are destemmed and seeded, cut into thin slices with a thickness of 1mm, and dried with hot air at 40°C until the water content is 10%. Dried hawthorn slices are pulverized, passed through a 40-mesh sieve, and the under-sieve is the prepared hawthorn powder.

[0033] 2. Extraction and separation of hawthorn pectin

[0034] The hawthorn powder 10g prepared in step 1) and 200mL of 95% ethanol were mixed and washed continuously for 1h, centrifuged at 5000r / min for 10min, the supernatant was discarded, and the precipitate was repeatedly washed with 200mL of 95% ethanol (to remove non- cell wall components). Add acetone to the precipitate after washing with ethanol, the mass volume ratio of the precipitate and acetone is 1:1...

Embodiment 2

[0043] The present embodiment selects Shandong area main product hawthorn variety-cotton ball (MI) as test raw material, and concrete implementation steps are as follows:

[0044] 1. Preparation of hawthorn powder

[0045] Fresh hawthorn fruit is destemmed and seeded, cut into thin slices with a thickness of 1mm, and dried with hot air at 40°C until the moisture content is 8.9%. Dried hawthorn slices are pulverized, passed through a 40-mesh sieve, and the under-sieve is the prepared hawthorn powder.

[0046] 2. Extraction and separation of hawthorn pectin

[0047] Mix 20 g of the hawthorn powder prepared in step 1) with 200 mL of 95% ethanol, continue stirring and washing for 2 h, centrifuge at 5000 r / min for 10 min, discard the supernatant, and repeat washing with ethanol of the same concentration (95%) and volume (200 mL) Precipitate to remove non-cell wall components. Add acetone to the precipitate washed with ethanol, the mass volume ratio of the precipitate to acetone ...

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Abstract

The invention relates to a preparation method of low-methoxy hawthorn pectin, in particular to a method for extracting, separating and purifying hawthorn pectin from hawthorn, namely a method for preparing low-methoxy hawthorn pectin (LMP) by taking amylase and amyloglucosidase as processing aids. Pectin esterase treatment is not used in the implementation process of the method, the pectin prepared through the method is high in purity, high in galacturonic acid content and large in linear polygalacturonic acid area proportion, and the molecular weight of the pectin is remarkably lower than that of hawthorn pectin prepared without the purification treatment process. The method is suitable for preparing the LMP by taking all types of hawthorns and processing byproducts thereof as raw materials, and is not influenced by the varieties, producing areas and maturity of the hawthorns, hawthorn processing byproducts comprise hawthorn waste residues obtained after hawthorn beverage processing,hawthorn waste residues obtained after hawthorn wine processing and the like, waste can be turned into wealth, and social and economic benefits are remarkable.

Description

[0001] 1. Technical field [0002] The invention relates to a preparation method of hawthorn pectin, which is notably characterized in that the prepared hawthorn pectin is low methoxyl pectin (LMP), and the method belongs to the technical field of food intensive processing. [0003] 2. Background technology [0004] Hawthorn, also known as shanlihong, red fruit and annatto, belongs to the Rosaceae plant. It is a unique fruit tree native to my country and is also planted in parts of Asia and Europe. At present, there are 18 varieties in my country, mainly distributed in Shandong, Henan, Hebei and other places. Hawthorn is resistant to cold and drought. It is a plant with the same source of medicine and food, and it can also be used as an ornamental plant. In addition to being rich in protein, carbohydrates, phenols, organic acids, vitamins and trace elements, hawthorn fruit has a particularly prominent pectin content, which is significantly higher than other fruit and vegetable...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/06G01N1/28G01N1/34G01N1/40A23L29/231
CPCA23V2002/00A23L29/231C08B37/0045G01N1/28G01N1/34G01N1/40G01N1/4005G01N1/4022G01N1/4044G01N2001/2893A23V2250/50724A23V2300/14A23V2300/40
Inventor 郭萌萌殷燕靖张召全马霞徐康朱传合
Owner SHANDONG AGRICULTURAL UNIVERSITY
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