Method for manufacturing collagen from black sea cucumbers from East China Sea

A technology of black cucumber collagen and manufacturing method, which is applied in fermentation and other directions, and can solve the problems of high price and limited production of sea cucumber collagen

Active Publication Date: 2011-11-23
宁波超星海洋生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] 3. At present, the main domestic species of sea cucumber - sea cucumber is expensive. In Liaoning and Shandong, the main producing areas of sea cucumber, the price of cultivated light-dried sea cucumber is 6,000-7,000 yuan per 500 grams, and the price of wild light-dried sea cucumber is even higher. 18,000-30,000 yuan per 500 grams, expensive raw material prices limit the production of sea cucumber collagen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: The steps of the manufacturing method of Donghai black ginseng collagen described in the present invention are as follows:

[0025](1) Firstly, the decontaminated and cleaned Donghai black ginseng (see the inventor’s authorized patent for the treatment method: A processing method of Donghai sea cucumber, ZL 2009 1 0098389 .7) is ground into a slurry with a stainless steel refiner , pass through a 40-mesh screen, add to the stainless steel enzymatic hydrolysis tank, add tap water 1.8-2.0 times the weight of Donghai black ginseng, turn on the enzymatic hydrolysis tank mixer, the speed is 30 rpm, open the enzymatic hydrolysis tank jacket to heat the steam, and put the material After heating to 95°C, keep stirring and heat for half an hour, turn off the steam, turn on the cooling water of the jacket of the enzymolysis tank to lower the temperature of the material to 45°C, and add 0.1% Alcalase hydrolyzing protease and 0.1% Protamex compound protease, 0.1% Flav...

Embodiment 2

[0029] Embodiment 2: The manufacturing method steps of Donghai black ginseng collagen of the present invention are as follows:

[0030] (1) Firstly, the decontaminated and cleaned Donghai black ginseng (see the inventor’s authorized patent for the treatment method: A processing method of Donghai sea cucumber, ZL 2009 1 0098389 .7) is ground into a slurry with a stainless steel refiner , pass through a 40-mesh screen, add to the stainless steel enzymatic hydrolysis tank, add tap water 1.8-2.0 times the weight of Donghai black ginseng, turn on the enzymatic hydrolysis tank mixer, the speed is 30 rpm, open the enzymatic hydrolysis tank jacket to heat the steam, and put the material After heating to 95°C, keep stirring and heat for half an hour, turn off the steam, turn on the cooling water of the jacket of the enzymolysis tank to lower the temperature of the material to 45°C, and add 0.1% Alcalase hydrolyzing protease and 0.1% Protamex compound protease, 0.1% Flavourzyme flavor p...

Embodiment 3

[0033] Embodiment 3: The manufacturing method steps of Donghai black ginseng collagen of the present invention are as follows:

[0034] (1) Firstly, the decontaminated and cleaned Donghai black ginseng (see the inventor’s authorized patent for the treatment method: A processing method of Donghai sea cucumber, ZL 2009 1 0098389 .7) is ground into a slurry with a stainless steel refiner , pass through a 40-mesh screen, add to the stainless steel enzymatic hydrolysis tank, add tap water 1.8-2.0 times the weight of Donghai black ginseng, turn on the enzymatic hydrolysis tank mixer, the speed is 30 rpm, open the enzymatic hydrolysis tank jacket to heat the steam, and put the material After heating to 95°C, keep stirring and heat for half an hour, turn off the steam, turn on the cooling water in the jacket of the enzymolysis tank to lower the temperature of the material to 45°C, and add 0.15% Alcalase hydrolyzing protease and 0.15% Protamex compound protease, 0.15% Flavourzyme flavo...

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PUM

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Abstract

The invention relates to a method for manufacturing collagen from black sea cucumbers from East China Sea, which comprises the following steps: (1) adding Alcalase hydrolysis protease in an amount which is 0.01 to 10 percent based on the weight of black sea cucumbers from East China Sea, protamex composite protease in an amount which is 0.01 to 10 percent based on the weight of black sea cucumbers from East China Sea and flavourzyme in an amount which is 0.01 to 10 percent based on the weight of black sea cucumbers from East China Sea into the black sea cucumbers from East China Sea which are subjected to foul-removing and cleaning treatment, heating the materials to 45 to 47 DEG C, and hydrolyzing at a constant temperature of 45 to 47 DEG C with stirring for 4 hours; and (2) after enzyme hydrolysis is finished, performing fine filtration by a membrane filter, concentrating in a vacuum concentrator, keeping the temperature of the materials in the vacuum concentrator between 55 and 58 DEG C by controlling the heating steam valve of the vacuum concentrator, and concentrating to obtain pasty or powder collagen product from black sea cucumbers from East China Sea. The method has the advantages that: a technical route of combination of three enzymes and hydrolysis degree intermediate detection is adopted, so the relative molecular weight of the product of the enzyme hydrolysis of the black sea cucumbers from East China Sea can be controlled to meet the requirement on the relative molecular weight of sea cucumber collagen products; and the product of the hydrolysis product is free from bitter taste and fishy smell.

Description

technical field [0001] The invention relates to the field of utilization and processing of new marine biological resources, in particular to a method for producing collagen from black ginseng of the East China Sea. Background technique [0002] According to research, there are about 140 kinds of sea cucumbers in my country. Among them, more than 20 kinds of sea cucumbers are edible. The common edible sea cucumbers include sea cucumbers, sea cucumbers, green sea cucumbers, jade-footed sea cucumbers, plum-flowered ginseng, white anal sweet potato (Acaudina leucoprocta H.L.Clark), white ginseng, and white ginseng. Radix Ginseng, Radix Ginseng, Radix Radix Ginseng with White Background, Radix Radix Radix Ginseng, Black Milk Ginseng, Coarse Sea Cucumber, Two-color Table Ginseng, etc. The black ginseng of the East China Sea is mainly composed of wild black sweet potatoes (also known as: sea cucumbers of the East China Sea or sweet ginseng of the East China Sea). The maximum body ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 章元炳叶再府叶再镯
Owner 宁波超星海洋生物制品有限公司
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