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Culturing method of sea source bacillus subtilis insect killing activity product and its application

A technology of Bacillus subtilis and insecticidal activity, which is applied in the field of cultivation of marine source Bacillus subtilis insecticidal activity products, can solve problems such as no reports, achieve good control effect, simple fermentation conditions, environmental and non-target biological safety Effect

Inactive Publication Date: 2004-12-22
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the development of agricultural insecticidal active substances from marine microorganisms has not been reported so far.

Method used

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  • Culturing method of sea source bacillus subtilis insect killing activity product and its application
  • Culturing method of sea source bacillus subtilis insect killing activity product and its application
  • Culturing method of sea source bacillus subtilis insect killing activity product and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Screening method for a marine source insecticidal microorganism

[0015] 1. The culture conditions of marine microorganisms to be tested: medium composition: add 2.0% soluble starch, 1.5% soybean powder, 0.5% yeast extract, 0.2% peptone, carbonic acid Calcium 0.2%, glucose 0.5%, and adjusted to pH 7.5-8.0; inoculum size: 1% seed solution equivalent to the total amount of the medium, cultured at 28°C, 200r / min shaker for 120h. The fermentation broth was centrifuged, and the supernatant was taken for testing the insecticidal activity.

[0016] 2. Conventional insect soaking screening method: take the 3rd instar cotton bollworm larvae that have been continuously raised indoors for multiple generations, soak them in the fermentation liquid stock solution for 30s, place them in finger tubes (one head per tube) with feed, and soak the blank controls separately. Clean water, soaked in fermentation medium, 20 3rd instar larvae per treatment. After treatment, place ...

Embodiment 2

[0021] Example 2: Fermentation and cultivation method of a marine source insecticidal microorganism

[0022] 1. Determination of fermentation time: Inoculate 1% seed liquid in the culture medium, ferment for 1d, 2d, 3d, 4d respectively, measure the activity of the fermentation broth, and determine the relationship between fermentation time and activity (see figure 1 ).

[0023] 2. Carbon source experiment: use 3.5% corn flour, 3.5% soluble starch, 3.5% maltose, 3.5% sucrose, 3.5% glucose, and 3.5% lactose to replace soluble starch and glucose in the medium, ferment for 3 days, and test the activity of cotton bollworm (See figure 2 ).

[0024] 3. Nitrogen source experiment: 1.7% soybean powder, 1.7% peptone, 1.7% beef extract, 1.7% yeast extract (imported), and 1.7% concentrated yeast powder (domestic) were used as nitrogen sources to replace soybean powder in the medium respectively Yeast extract, fermented for 3 days, assayed by cotton bollworm (see image 3 ).

[0025]...

Embodiment 3

[0049] Example 3: Determination of insecticidal spectrum of a marine source insecticidal microbial fermentation product (see Table 3 for specific results)

[0050] 1. Insect soaking method Put the 3rd instar larvae of cotton bollworm, cabbage worm, cabbage worm and armyworm continuously raised in the room into the concentrated solution of JAAS01 fermentation broth for 30s, and then insert them into fresh cotton, cabbage, small green vegetables, In the petri dishes of cabbage and corn leaves (petioles containing 0.2% NaNO 3 , 0.1%K 2 HPO 4 20 third-instar larvae per dish, each treatment was repeated 3 times, and after treatment, the temperature was 25±0.5°C, the photoperiod L:D=14:10, and the relative humidity was 75%-85°C. % raised in the incubator. 48h to check the larval mortality.

[0051] 2. Leaf soaking method: Cut the leaves of cabbage with aphids aphids into small pieces, put them into the JAAS01 fermented broth concentrate and soak them for 10 seconds, absorb exces...

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Abstract

A process for culturing the Bacillus subtilis coming from sea to obtain the insecticiding product for more agricultural pests includes bioactive screening to the marine microbes in Chinese sea to obtain a high-activity Bacillus subtilis strain, and culturing it in fermenting culture medium to obtain said active insecticiding product. Its advantages are high killing activity to agricultural pests, broad spectrum, and no environmental pollution.

Description

Technical field: [0001] The invention relates to a cultivation method and application of an insecticidal active product of Bacillus subtilis, in particular to a cultivation method and application of an insecticidal active product of marine source Bacillus subtilis. Background technique: [0002] Pesticides play an important role in human resistance to pests and diseases, and in protecting crops with high quality and high yield. Since the advent of organic synthetic pesticides in the middle of the 20th century, they once played a huge role in promoting agricultural production due to their high efficiency, quick effect, and broad spectrum. However, due to the large-scale use of highly toxic and high-residue organic synthetic pesticides, in just a few decades, the negative impacts have been far beyond people's expectations, such as pest resistance, environmental pollution, and mass killing of beneficial organisms. etc. The development of natural product resources with insecti...

Claims

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Application Information

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IPC IPC(8): A01N63/02C12N1/20
Inventor 刘贤金余向阳刘济宁王冬兰张存政
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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