Marine-microbe-sourced low-temperature beta-galactosidase, and coding gene thereof and application thereof

A technology of galactosidase and marine microorganisms, applied in the direction of plant genetic improvement, application, genetic engineering, etc., can solve problems such as limited application, high optimum temperature, and rapid inactivation
CN103131685AInactive Publication Date: 2013-06-05SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Publication Date
2013-06-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the field of marine biotechnology, and provides a marine-microbe-sourced low-temperature beta-galactosidase, which is obtained by fermentation culturing and purification of a marine halomonas sp. strain P6009-1 obtained by the applicant previously. The marine halomonas sp. strain P6009-1 is cultured and separated from seawater in East China Sea areas, and has a collection number of CCTCC No: M2011001. Under a temperature of 0 DEG C, a capacity of the enzyme in catalyzing lactose hydrolysis is 20% of a maximal activity, such that good low-temperature performance is shown. The invention further provides a coding gene of beta-galactosidase, and an application of beta-galactosidase in decomposing milk lactose.
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Description

technical field

[0001] The invention relates to the technical field of marine organisms, in particular to a low-temperature beta-galactosidase derived from marine microorganisms, its coding gene and its application. Background technique

[0002] β-galactosidase (scientific name β-D-galactoside galactohydrolase, β-D-galactoside galatohydrolase, EC3.2.1.23) is a specific hydrolysis of β-1,4-glucoside It is a key hydrolase that can hydrolyze lactose into glucose and galactose, and also has the function of galactoside transfer.

[0003] β-galactosidase is widely found in organisms in nature, such as plants, bacteria, fungi (Aspergillus oryzae, Aspergillus niger, Saccharomyces brittle, lactic acid yeast), actinomycetes, and animal intestines (especially infant intestines) middle.

[0004] Judging from the catalytic reaction, β-galactosidase belongs to glycosyl hydrolase (abbreviated as GH). According to its sequence and structure, β-galactosidase is divided into four families, ...

Claims

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