The invention discloses a preparation method of a mineral supplement preparation. The mineral supplement preparation comprises the following components in parts by weight of 15-30 parts of eggshell, 30-40 parts of soybean meal, 0.2-0.5 part of radix astragali, 0.1-0.4 part of folium isatidis, 10-18 parts of malaytea scurfpea fruits, 6-12 parts of collagen, 1-3 parts of zinc methionine, 1-3 parts of cupric glycinate, 1-3 parts of ferrous fumarate, 1-3 parts of selenium-enriched malt, 1-3 parts of polydextrose, 1-3 parts of complex vitamin B and 1-3 parts of vitamin C. The mineral supplement preparation disclosed by the invention has the beneficial effects that through microbe and enzyme glycolysis, substances of Chinese herbal medicine effective components, active peptide, vitamins, and thelike are effectively combined, so that the solubility and the absorptivity of minerals are obviously strengthened. The preparation can obviously promote organisms for absorbing minerals of calcium, selenium, ferrum and the like, the biological effectiveness is increased, skeleton formation and mineralization are promoted, the bone weight and the ash content are increased, and chronic diseases ofcardiovascular disease, diabetes, cataract and the like can be effectively prevented.