Method for grading molecular weight of anglerfish skin collagen peptide

A technology of fish skin collagen and collagen peptide, which is applied in the field of fish collagen peptide, can solve the problem that collagen peptide cannot be effectively graded, and achieve the effect of improving the extraction rate and enhancing the efficiency of nanofiltration

Inactive Publication Date: 2014-03-12
ZHEJIANG MARINE DEV RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to overcome the disadvantages that collagen peptides with different molecular weights can not be effectively graded due to the preparat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 1000 g of cleaned monkfish skin, and cut it into pieces with a length and width of about 2 cm×2 cm with scissors. Add 30L of 5% NaOH solution to monkfish skin fragments, soak for 24 h, take out, and then wash with water until the pH is neutral. Then add 40L of 10% n-butanol solution to the fish skin, stir for 28 h, take it out, wash with water and drain. Mash the drained monkfish skin fragments, add 5% acetic acid solution with a mass fraction of 1:5 according to the ratio of fish skin to acetic acid solution, heat to 60°C, and continuously stir and extract for 5 h , to obtain crude collagen extract after filtration. Then the crude collagen extract was put into an insulating bag, and subjected to high-voltage pulse electric field treatment for 0.5h. Set the pulse time of the high-voltage pulsed electric field treatment as 1s, the pulse frequency as 30Hz, and the electric field intensity as 25kV / cm. After taking it out, add 1% pepsin to the crude collagen extract...

Embodiment 2

[0025]Take 1000 g of cleaned monkfish skin, and cut it into pieces with a length and width of about 2 cm×2 cm with scissors. Add 30L of 5% NaOH solution to monkfish skin fragments, soak for 24 h, take out, and then wash with water until the pH is neutral. Then add 40L of 10% n-butanol solution to the fish skin, stir for 28 h, take it out, wash with water and drain. The drained monkfish skin fragments were mashed, and the ratio of fish skin to acetic acid solution was 1:5, adding 5% acetic acid solution, heated to 80°C, and continuously stirred for 5 h , to obtain crude collagen extract after filtration. Then the crude collagen extract was put into an insulating bag, and subjected to high-voltage pulse electric field treatment for 1 hour. Set the pulse time of the high-voltage pulsed electric field treatment as 1s, the pulse frequency as 40Hz, and the electric field intensity as 15kV / cm. After taking it out, add 2% pepsin to the crude collagen extract based on the mass of th...

Embodiment 3

[0027] Take 1000 g of cleaned monkfish skin, and cut it into pieces with a length and width of about 2 cm×2 cm with scissors. Add 30L of 5% NaOH solution to monkfish skin fragments, soak for 24 h, take out, and then wash with water until the pH is neutral. Then add 40L of 10% n-butanol solution to the fish skin, stir for 28 h, take it out, wash with water and drain. The drained monkfish skin fragments were mashed, and the ratio of fish skin to acetic acid solution was 1:5, and 5% acetic acid solution was added, heated to 70°C, and continuously stirred for 5 h. , to obtain crude collagen extract after filtration. Then the crude collagen extract was put into an insulating bag, and subjected to high-voltage pulse electric field treatment for 0.8h. Set the pulse time of the high-voltage pulsed electric field treatment as 1s, the pulse frequency as 35Hz, and the electric field intensity as 20kV / cm. After taking it out, add 1.5% pepsin to the crude collagen extract based on the m...

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PUM

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Abstract

The invention discloses a method for grading the molecular weight of anglerfish skin collagen peptide. The method comprises the following steps: soaking anglerfish skin in dilute alkaline liquid; taking out, then washing with water and draining; performing enzymolysis on the anglerfish skin; after deactivating enzyme, adding active carbon for debitterizing and decoloring; entrapping collagen peptide with the molecular weight being more than 10,000 Daltons from the filtrate obtained after the active carbon is filtered out, by a first-level plate type ultrafiltration membrane; entrapping collagen peptide with the molecular weight being more than 2,000 Daltons by a second-level roll type ultrafiltration membrane; and entrapping collagen peptide with the molecular weight being more than 200 Daltons by a roll type ultrafiltration membrane. According to the method, the anglerfish skin is hydrolyzed by using pepsase and flavourzyme step by step during enzymolysis, and the collagen peptide with the molecular weight in broad distribution is subjected to effective molecular weight grading processing after enzymolysis, so the collagen peptide with different molecular weight segments can be obtained and can be effectively utilized.

Description

technical field [0001] The invention relates to the technical field of fish collagen peptides, in particular to a method for grading the molecular weight of ankang fish skin collagen peptides. Background technique [0002] Monkfish is a cold-temperate bottom fish widely distributed in the eastern waters of my country (the northern part of the East China Sea, the Yellow Sea and the Bohai Sea). In recent years, with the increasing amount of monkfish processing in my country, a large amount of leftovers such as fish skin are left in the processing process, which pollutes the environment and causes a waste of resources. Relevant personnel have done relevant research on chondroitin sulfate, dermatan sulfate, collagen and other active substances in the by-products of monkfish processing. It has been found that the preparation of collagen peptides by hydrolyzing monkfish collagen can significantly improve its nutritional value and digestion. Its collagen peptide can maximize the ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 杨会成郑斌李八方马华威周宇芳
Owner ZHEJIANG MARINE DEV RES INST
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