Resistance identification method for potato black scurf
A technology for identification of potato black mole disease and resistance, applied in biochemical equipment and methods, microbe determination/inspection, measuring devices, etc., can solve problems such as scientific evaluation of potato variety black mole disease resistance, and achieve identification The effect of scientific method, clear classification and simple calculation process
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[0047] The technical solution of the present invention is further described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following description.
[0048] A method for identifying resistance to potato black mole disease, comprising the steps of:
[0049] S1 Isolation and preparation of pathogenic bacteria: collect black mole disease-infected potato pieces from the field, isolate black mole pathogenic bacteria according to the tissue separation method, and verify by back-grabbing potato pieces according to Koch's law;
[0050] S2 pathogen expansion: inoculate the nevus diseased strain obtained after verification in the S1 step on the potato dextrose agar medium (PDA), cultivate for 4 days, and obtain the Rhizoctonia solani bacteria cake for use;
[0051] S3 Preparation of Rhizoctonia solani inoculum: Add distilled water to wheat bran and stir evenly, autoclave at 121°C for 40 minutes, inoculate on t...
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