Resistance identification method for potato black scurf

A technology for identification of potato black mole disease and resistance, applied in biochemical equipment and methods, microbe determination/inspection, measuring devices, etc., can solve problems such as scientific evaluation of potato variety black mole disease resistance, and achieve identification The effect of scientific method, clear classification and simple calculation process

Inactive Publication Date: 2018-05-04
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the identification method of potato black mole disease resistance, and it is impossible to make a scientific evaluation of the resistance of potato varieties to black mole disease

Method used

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  • Resistance identification method for potato black scurf
  • Resistance identification method for potato black scurf
  • Resistance identification method for potato black scurf

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Embodiment Construction

[0047] The technical solution of the present invention is further described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following description.

[0048] A method for identifying resistance to potato black mole disease, comprising the steps of:

[0049] S1 Isolation and preparation of pathogenic bacteria: collect black mole disease-infected potato pieces from the field, isolate black mole pathogenic bacteria according to the tissue separation method, and verify by back-grabbing potato pieces according to Koch's law;

[0050] S2 pathogen expansion: inoculate the nevus diseased strain obtained after verification in the S1 step on the potato dextrose agar medium (PDA), cultivate for 4 days, and obtain the Rhizoctonia solani bacteria cake for use;

[0051] S3 Preparation of Rhizoctonia solani inoculum: Add distilled water to wheat bran and stir evenly, autoclave at 121°C for 40 minutes, inoculate on t...

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Abstract

The invention relates to a resistance identification method for potato black scurf. The resistance identification method comprises the following steps: S1: separating and purifying pathogenic bacteria; S2: carrying out pathogenic bacteria expanding propagation; S3: preparing an inoculum; S4: preparing sterile soil; S5: carrying out pathogenic bacteria inoculation and potato seed processing; S6: investigating items, and carrying out a calculation method: according to the lesion area of the potato tuber rhizoctonia solani, calculating an illness state index, calculating a relative disease-resistant index according to the illness state index, carrying out disease-resistant evaluation of a variety through the relative disease-resistant index, and determining the resistance level of the potatoblack scurf according to the relative disease-resistant index: immunity is that the relative disease-resistant index is equal to 1, high resistance is that the relative disease-resistant index is greater than or equal to 0.80 and less than or equal to 0.99, moderate resistance is that the disease-resistant index is greater than or equal to 0.60 and less than or equal to 0.79, moderate susceptibility is that the relative disease-resistant index is greater than or equal to 0.40 and less than or equal to 0.59, and high susceptibility is that the relative disease-resistant index is greater than orequal to 0.00 and less than or equal to 0.39. The resistance identification method has the advantages of being scientific, accurate in results, simple in processes, easy in operation and short in hypha culture time.

Description

technical field [0001] The invention relates to the technical field of identification of crop disease resistance, in particular to a method for identifying resistance to potato black mole disease. Background technique [0002] The potato industry gives full play to the advantages of the superior geographical environment, cool climate, long planting history, great potential for increasing production and good economic benefits in the southern mountainous area, with the core of "stabilizing the area, increasing the production and increasing the profit", insisting on the development of seed potatoes and the export of fresh potatoes Potato industry has become a pillar industry for speeding up the guarantee of food security, increasing farmers' income, government poverty alleviation and promoting the development of Ningxia's rural economy. Potato black spot disease is a soil-borne disease caused by Rhizoctonia solani, also known as Rhizoctonia solani, stem rot, Rhizoctonia canker,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18G01N2333/37
Inventor 王喜刚
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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