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Rapid multiplying medium of vanda protocorm

A proliferation medium, Vanda orchid technology, applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve problems such as reducing browning rate, achieve browning inhibition, inhibition of multiplication browning, and increase multiplication coefficient Effect

Inactive Publication Date: 2016-01-13
GUANGDONG YUESANPANG AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on this, it is necessary to solve the problem of severe browning of vanda protocorms during proliferation and affect the proliferation efficiency, and provide a vanda rapid proliferation medium that can inhibit browning, using MS medium as the base medium, and adding ZT (zeatin), 2,4-D (2,4-dichlorophenoxyacetic acid), reduced glutathione, agar powder, sucrose, can accelerate the proliferation of Vanda, increase the proliferation coefficient and inhibit the browning of proliferation, reduce browning rate
[0005] Based on this, it is necessary to provide a fast proliferation medium for vanda orchids that can inhibit browning in order to solve the problem of severe browning of protocorms of vanda orchids and affect the proliferation efficiency.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0019] Embodiment one: the preparation of vanda blue rapid proliferation medium of the present invention

[0020] According to the MS medium formulation shown in Table 1 and the Vanda rapid proliferation medium formulation shown in Table 2, the Vanda rapid proliferation medium of the present invention was prepared.

[0021] According to the amount of prepared 1100mL medium, weigh each component in MS medium, 2,4-dichlorophenoxyacetic acid, agar powder, and sucrose, respectively, put them in a conical flask, add an appropriate amount of purified water, stir to dissolve, and constant volume to 1000mL and adjust the pH to 6.5. Seal the triangular flask, place it in an autoclave, and sterilize it at 121°C for 20 minutes, then place it in an ultra-clean workbench and let it cool naturally to obtain a sterilized medium mother solution for future use.

[0022] According to the amount of preparing 1100mL medium, weigh zeatin and reduced glutathione respectively, add an appropriate am...

Embodiment 2

[0029] Embodiment two: the test of Vanda blue rapid proliferation medium of the present invention

[0030] According to the MS medium formula shown in Table 1 and the Vanda blue rapid proliferation medium formula shown in Table 3, with reference to the medium preparation method described in Example 1, respectively prepare the Vanda blue fast proliferation culture of each test group and control group base.

[0031] Table 3 Vanda blue rapid proliferation medium.

[0032]

[0033] Remarks: Control group 1 is the control group of the prior art, and its formula is the formula of conventional vanda blue proliferation medium.

[0034] Select the first-generation protocorms of Vanda Orchid without browning, vitrification, pollution, and healthy growth, cut into small pieces with a thickness of about 2-3 cm, and inoculate them in the culture medium of each test group and control group shown in Table 3. , 20 pieces were inoculated in each bottle, and then cultured under the conditi...

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Abstract

The invention discloses a rapid multiplying medium of vanda protocorm. The rapid multiplying medium of the vanda protocorm takes an MS medium as a basal medium, and ZT (zeatin), 2,4-D(2,4-dichlorphenoxyacetic acid), reduced glutathione, agar powder and saccharose are added. The multiplying speed of vanda can be increased, the multiplication coefficient is increased, multiplication and browning are restrained, and the browning rate is decreased.

Description

technical field [0001] The invention relates to the technical field of orchid tissue culture, in particular to a medium for rapid proliferation of vanda orchid protocorms. Background technique [0002] Vanda Orchid, also known as Vanda Orchid, belongs to the Orchidaceae Vanda Orchid. Vanda orchids have large flowers and bright colors, especially blue flowers. It can be used for potted plants or hanging pots for viewing, and can also be placed in the living room, study or hung on the windowsill or balcony. Due to its long flowering period and storage and transportation resistance, it is often used as cut flowers for viewing, making bridal bouquets and VIP ceremonial bouquets, and is deeply loved by people at home and abroad. [0003] In recent years, orchid tissue culture technology and industrial production of test-tube seedlings have achieved rapid development, but the browning problem of Vanda Orchid, especially the browning problem of protocorm proliferation, seriously ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 黄庆梅
Owner GUANGDONG YUESANPANG AGRI TECH CO LTD
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