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Preparation method of fish cartilage extracts and obtained product

A technology of extract and cartilage, which is applied in the field of preparation of fish cartilage extract, can solve the problems of raw material pollution threat steps, high active ingredient content, insufficient raw material utilization, etc., achieve simple process flow, high recovery rate, and improve raw material utilization value effect

Active Publication Date: 2010-05-26
SHANGHAI HUIWEN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the shortcomings of the existing methods for producing chondroitin sulfate and / or collagen peptides, such as insufficient utilization of raw materials, low active ingredients of the product, threat of contamination of raw materials or complicated steps, etc., and provide a Preparation method and product obtained from fish cartilage extract
The invention can not only solve the problem of shortage of collagen peptide and chondroitin sulfate in the market, truly turn waste into wealth, rationally and fully utilize natural resources, make the best use of everything, and reduce environmental pollution; moreover, the cost of raw materials is low, and the product safety performance is high , high content of active ingredients

Method used

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  • Preparation method of fish cartilage extracts and obtained product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take an appropriate amount of cleaned salmon (Oncorhynchnchus Spp.) head, heat in a reaction tank at 100°C for 10 minutes, separate 500 g of cartilage and pulverize it with a homogenizer, add 500 g of water, adjust the pH to 8.5 with 10% sodium hydroxide, and add 0.1 % Alcalase 3.0T alkaline protease at 65°C for 1 hour, adjust the pH to 6.5 with 1% hydrochloric acid, add 0.05% of raw material weight Novo-flavored protease at 65°C for 2 hours. Heat up to 95°C to kill enzymes, cool down to 70°C, centrifuge at 4000r / min for 30min, filter to remove impurities, add 80g of white coarse diatomaceous earth to aid in filtration, dilute the filtrate with water to 2 times the mass of the filtrate, and pass through a coiled membrane with a molecular weight cut-off of 5000 Molecular sieves, under the conditions of 50°C and a pressure of 2 kg / cm2, carry out molecular weight cutting, circulate and separate for 1 hour, collect the membrane permeate, concentrate it under reduced pressure...

Embodiment 2

[0036] Take an appropriate amount of cleaned Raja porosa, heat in a reaction tank at 85°C for 20 minutes to remove impurities, separate 500 g of cartilage and pulverize it with a homogenizer, add 500 g of water, adjust the pH to 7.5 with 10% sodium hydroxide, add cartilage 0.2% by weight of Alcalase 3.0T alkaline protease was hydrolyzed at 55°C for 0.5h, the pH was adjusted to 6.0 with 1% hydrochloric acid, and 0.2% by weight of cartilage Novo flavored protease was added to enzymolyze at 55°C for 1h. Heat up to 85°C to inactivate the enzyme, cool down to 50°C, centrifuge at 4000r / min for 30min, filter to remove impurities, and add 80g of kaolin to filter. Dilute the filtrate with water to 2 times the quality of the filtrate, pass through a tubular membrane molecular sieve with a molecular weight cut-off of 5000, cut the molecular weight under the conditions of 70°C and a pressure of 2 kg / cm2, circulate and separate for 1.5h, collect the membrane permeate and concentrate it unde...

Embodiment 3

[0039] Take an appropriate amount of cleaned shark (prionace glauca (Linnaeus)) bone, heat it in a water bath at 75°C for 30 minutes to remove impurities, separate 500 g of cartilage and pulverize it with a homogenizer, add 600 g of water, and adjust the pH to 9 with 10% sodium hydroxide. Add 0.25% of raw material weight Alcalase 2.4L alkaline protease for enzymolysis at 50°C for 0.5h, adjust the pH to 6.8 with 1% hydrochloric acid, add 0.25% raw material weight of Novo flavor protease for enzymolysis at 50°C for 0.5h. Heat up to 80°C to kill the enzyme, cool down to 45°C, centrifuge at 2000r / min for 40min, filter to remove impurities, add 80g of diatomaceous earth to help filter, dilute the filtrate with water to 2 times the quality of the filtrate, pass through a hollow fiber membrane molecular sieve with a molecular weight cut off of 15000 Under the condition of lower than 50°C and pressure of 1.5 kg / cm2, carry out molecular weight cutting, circulate and separate for 2 hours...

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Abstract

The invention discloses a preparation method of fish cartilage extracts and an obtained product, comprising the following steps: carrying out alkaline protease enzymolysis on fish cartilage to obtain enzymolysis liquid, carrying out flavorzyme enzymolysis, inactivating enzyme and removing impurities to obtain the fish cartilage extract. The invention overcomes the defects that the existing methodfor producing chondroitine and / or collagen peptide can not fully utilize the raw materials, the product has low effective components, the raw materials are threatened by pollution or the steps are complex and the like. The invention provides the preparation method of fish cartilage extracts and the obtained product. The invention can acquire lacking products in the market by a simple technical process, further improves use value of the raw materials, not only can solve the problem of lacking collagen peptide in the market and chondroitine, but also change waste material into useful things, reasonably and fully utilize natural resources, make the best use of things and reduce environmental pollution; the invention has the advantages of low raw material cost, high product security and highcontent of effective components.

Description

technical field [0001] The invention relates to a preparation method of fish cartilage extract and the obtained product. Background technique [0002] Collagen peptide is the main component of connective tissue in the human body. It is the constituent material of the skin, bones, tendons, cartilage, and blood vessels of the human body, accounting for one-third of the total protein in the body. The protein digestion and absorption rate of collagen peptides is almost 100%. According to literature reports, collagen peptides have many functions such as protecting gastric mucosa, promoting skin collagen metabolism, inhibiting blood pressure, promoting calcium absorption, and reducing serum cholesterol levels. Therefore, collagen peptides Peptides are widely used in various fields such as medicine, food, beauty, feed production, chemical industry, etc., and are materials in short supply in the market. [0003] Chondroitin sulfate is generally a natural acidic mucopolysaccharide e...

Claims

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Application Information

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IPC IPC(8): A23L1/327A23J3/04A23L1/30A23L1/305A23L33/10
Inventor 刘桂云骆峰骆滨
Owner SHANGHAI HUIWEN BIO TECH
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