Method for preparing collagen peptide by using cod skin

A technology of skin collagen and protein peptides, applied in the field of preparation of collagen peptides, can solve the problems of safe existence of collagen, easy to pollute the environment, and low added value, and achieve the effects of easy digestion and absorption, no environmental pollution, and low equipment investment

Inactive Publication Date: 2009-04-15
TAIXIANG GRP TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the safety hazards of collagen prepared from terrestrial animals in the prior art, and the waste of cod skin or as a raw material for producing fish meal, the resulting low added value and easy pollution of the environment, the present...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Take fresh or thawed cod skin 0.5Kg, wash 4-5 times with 2L water, drain; take 1L water, add 0.1% Ca(OH) of water weight 2 Make a mixed solution with 0.2% NaOH, soak the cod skin in the soaking solution for 10 hours, filter and remove the supernatant, wash the sediment repeatedly with water to make the pH value neutral, and add 1L of glacial acetic acid with a concentration of 0.4% , soaked for 2 hours, filtered to remove the supernatant, and the precipitate was washed repeatedly with water until the pH value was neutral. Heat the distilled water to 75°C, add 2L of distilled water to extract for 9 hours under constant temperature stirring, remove insoluble matter by filtration, cool the filtrate to about 48°C, adjust the pH value to 7 with NaOH, and add 0.3g of neutral protease, hydrolyzed for 4 hours, inactivated at 100°C for 5 minutes, concentrated in vacuo, and finally spray-dried to obtain collagen active peptide powder.

[0012] The obtained collagen active peptid...

Embodiment 2

[0014] Take 1Kg of fresh or thawed cod skin, wash it 4-5 times with 4L of water, drain it; take 2L of water, add 0.05% Ca(OH) of water weight 2 Make a mixed solution with 0.15% NaOH, soak the cod skin in the soaking solution for 14 hours, filter and remove the supernatant, wash the sediment repeatedly with water to make the pH value neutral, and add 0.3% glacial acetic acid 2L , soaked for 3 hours, filtered to remove the supernatant, and the precipitate was washed repeatedly with water until the pH value was neutral. Heat the distilled water to 75°C, add 4L of distilled water to extract for 8 hours under constant temperature stirring, remove insoluble matter by filtration, cool the filtrate to about 48°C, adjust the pH value to 7 with NaOH, and add 1.5g of alkaline protease, hydrolyzed for 3 hours, inactivated at 100°C for 5 minutes, concentrated in vacuo, and finally spray-dried to obtain collagen active peptide powder.

Embodiment 3

[0016] Take fresh or thawed cod skin 3Kg, wash 4-5 times with 12L water, drain; take 6L water, add 0.2% Ca(OH) of water weight 2 Make a mixed solution with 0.3% NaOH, put the cod skin in the soaking solution and soak for 8 hours, filter and remove the supernatant, wash the sediment repeatedly with water to make the pH value to neutral, add 6L of glacial acetic acid with a concentration of 0.6%, Soak for 1 hour, filter to remove the supernatant, and wash the precipitate with water repeatedly until the pH value is neutral. Heat the distilled water to 75°C, add 12L of distilled water to extract for 10 hours under constant temperature stirring, remove insoluble matter by filtration, cool the filtrate to about 48°C, adjust the pH value to 7 with NaOH, and add 3g of bromelain, hydrolyzed for 5 hours, inactivated at 100°C for 5 minutes, concentrated in vacuo, and finally spray-dried to obtain collagen active peptide powder.

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Abstract

The invention relates to a method for preparing a collagen peptide by taking a cod skin as a material; in the method, the cod skin is selected as the material and is washed clearly by fresh water; 0.2 percent-0.5 percent mixed liquid of calcium hyduloxide and sodium hydroxide is added for dipping; then the cod skin is washed by fresh water until the pH value is neutral; after the water liquid of 0.3 percent to 0.6 percent diluted acid is added for dipping, the cod skin is washed by fresh water again until the pH value is neutral; the cod skin is cracked and is stirred and extracted in hot water for extracting a collagen; then undissolved substances are removed through filtering; the pH value of the extraction liquid of the collagen is adjusted to be neutral; under the stirring conditions, enzymes are added for carrying out zymohydrolysis and obtaining a zymohydrolysis liquid; and after the enzymes are eliminated and filtering is carried out, the powder of the collagen peptide is obtained through vacuum condensing and sponging drying. The method has the advantages of simple technique, less device investment, no environment pollution and being suitable for industrialization production. The collagen content of the powder of the prepared collagen peptide is higher; and the color, smell and biological properties are excellent. The powder of the collagen peptide can be broadly applied to the industries of health-care foods, medicines, and the like.

Description

technical field [0001] The invention relates to a method for preparing peptides with indefinite number of amino acids from fish, in particular to a method for preparing collagen peptides from cod skin. Background technique [0002] Collagen, also known as collagen, is a helical fibrous protein twisted by three peptide chains. It is a glycoprotein, and its molecules contain sugar and a large amount of glycine, proline, hydroxyproline, etc. It exists in all multicellular In animals, it is the most abundant type of protein in the body. It accounts for about a quarter of the total protein content in the human body. It exists in almost all tissues. It is an extracellular protein that exists in the form of insoluble fiber. It is harmful to animals and The formation of human skin, blood vessels, bones, bones, and cartilage is very important. It is an important substance of connective tissue and the main factor determining the toughness of connective tissue. It plays the role of bon...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14
Inventor 刘扬瑞郭玉华李振兴李可伟
Owner TAIXIANG GRP TECH DEV
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