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Combined production method of hyaluronic acid, chondroitin sulfate, collagen peptide, bone meal fodder and soap

A chondroitin sulfate and collagen peptide technology, applied in the fields of chondroitin sulfate, bone meal feed and soap co-production, hyaluronic acid, and collagen peptides, can solve problems such as hidden dangers of ethanol safety, achieve significant economic benefits and increase profit margins Effect

Active Publication Date: 2015-04-22
CHANGSHU INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved in the present invention is to provide a method for the joint production of hyaluronic acid, chondroitin sulfate, collagen peptide, bone meal feed and soap, which co-produces hyaluronic acid, chondroitin sulfate, collagen peptide, Bone meal feed and soap, abandoning the traditional method of precipitating chondroitin sulfate with ethanol, using resin adsorption-spray drying method to prepare chondroitin sulfate, which solves the hidden safety problems of ethanol in the production process; using compound enzyme step-by-step enzymatic hydrolysis technology, The complete separation of chondroitin sulfate from protein not only increases the product content, but also saves the hydrogen peroxide oxidation step

Method used

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  • Combined production method of hyaluronic acid, chondroitin sulfate, collagen peptide, bone meal fodder and soap
  • Combined production method of hyaluronic acid, chondroitin sulfate, collagen peptide, bone meal fodder and soap
  • Combined production method of hyaluronic acid, chondroitin sulfate, collagen peptide, bone meal fodder and soap

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Crush 1000kg of shark cartilage (including wing bones, vertebrae and skulls), add 4000kg of distilled water, raise the temperature to 75°C, keep it for 1h, cool down to 55°C, adjust the pH to 8.0 with 400g / L NaOH solution, and then add 1×10 9 U complex protease A, hydrolyze at 55°C for 2 hours; adjust the pH to 7.0, then add 4.0×10 8 U complex protease B, hydrolyze at 52°C for 4h; adjust the pH to 6.5 with hydrochloric acid, raise the temperature to 80°C, and keep for 0.5h to inactivate the enzyme. After the enzymolysis is completed, the enzymolysis solution is filtered with a plate-and-frame leaf filter while it is hot, and the filtrate and bone residue-denatured protein complex are obtained respectively. The bone residue-denatured protein complex was vacuum dried and pulverized to obtain 348 kg of bone residue-denatured protein complex powder. After adjusting the pH of the filtrate to 5.0 with hydrochloric acid, put it into a special adsorption resin column for glyco...

Embodiment 2

[0078] Soak 1000kg of bovine and pig cartilage (including nasal bone, larynx and trachea) in water to soften it, then crush it, add 5000kg of distilled water, raise the temperature to 80°C, keep it for 2h, remove the upper layer of bone oil, cool down to 55°C, and adjust with NaOH pH to 8.5, then add 1.4×10 9 U complex protease A, hydrolyze at 55°C for 2h; adjust the pH to 7.0, then add 6.0×10 8 U complex protease B, hydrolyze at 50°C for 5h; adjust the pH to 6.5 with hydrochloric acid, raise the temperature to 80°C, and keep for 0.5h to inactivate the enzyme. After the enzymolysis is completed, the enzymolysis solution is filtered with a plate-and-frame leaf filter while it is hot, and the filtrate and bone residue-denatured protein complex are obtained respectively. The bone residue-denatured protein complex was vacuum dried and pulverized to obtain 121 kg of bone residue-denatured protein complex powder. After adjusting the pH of the filtrate to 5.0 with hydrochloric acid...

Embodiment 3

[0094] Other operating steps are all the same as in Example 2, the difference is that the raw materials used are replaced with various ingredients shown in Table 5, and the theoretical and actual yields of chondroitin sulfate and collagen peptides are calculated respectively, from the table It can be seen from the figure that the yield of chondroitin sulfate and collagen peptide prepared by the present invention is close to the theoretical yield and the yield is high.

[0095] Table 5 Theoretical and actual yields of chondroitin sulfate and collagen peptides from different raw materials

[0096]

[0097] Note: The actual yield of chondroitin sulfate above refers to the yield after removing hyaluronic acid.

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Abstract

The invention discloses a combined production method of hyaluronic acid, chondroitin sulfate, collagen peptide, bone meal fodder and soap. The method mainly comprises the process steps that animal cartilage is soaked with water and smashed to obtain cartilage powder, the cartilage powder undergoes protease enzymolysis and then is adsorbed by resin, adsorbed enzymatic hydrolysate is collected, the resin undergoes gradient elution through a NaCl aqueous solution, and eluants undergo desalination, concentration, sterilization and drying to obtain finished products of the hyaluronic acid and chondroitin sulfate; the adsorbed enzymatic hydrolysate undergoes decoloration, anion and cation exchange resin mixed column treatment, nanofiltration purification, concentration, sterilization and drying to manufacture finished products of the collagen peptide; grease undergoes extraction saponification to manufacture the soap; denatured protein-bone dreg compounds and other materials are mixed to manufacture the fodder. According to the combined production method, by utilizing the process of resin adsorption-spray drying, highly-intensified resource utilization of cartilage is achieved, energy saving and emission reduction are achieved, and the economic benefit and the social benefit are remarkable.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for co-production of hyaluronic acid, chondroitin sulfate, collagen peptide, bone meal feed and soap. Background technique [0002] my country is a large agricultural country with relatively developed animal husbandry and rich cartilage resources. Active ingredients such as chondroitin sulfate, collagen and hyaluronic acid rich in animal cartilage have been widely used in the fields of medicine, food and cosmetics. [0003] Hyaluronic acid (Hyaluronic Acid, HA) and chondroitin sulfate (Chondroitin Sulfate, CS) are both glycosaminoglycans (Glyeosaminoglyean, GAG), and chondroitin sulfate is a copolymer of D-glucuronic acid and N-acetylgalactosamine The sulfate ester salt of the copolymer, the six-carbon sugar in the copolymer is connected alternately by β-(1,4) and β-(1,3) glycosidic bonds, generally contains about 50-70 disaccharide units, and the molecular weig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/22C07K1/18C08B37/08C11D9/02C11D13/22A23K1/10A23K1/14C02F9/04
Inventor 吴凌天吴敬源吴林元
Owner CHANGSHU INSTITUTE OF TECHNOLOGY
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