Method for producing chondroitin sulfate with coproduction of hydrolyzed collagen by employing fish cartilage

A technology for hydrolyzing collagen and chondroitin sulfate, which is applied in the chemical industry to achieve comprehensive utilization, remarkable economic and social benefits

Inactive Publication Date: 2015-02-25
QINGDAO BETTER BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, considering the comprehensive utilization of fish cartilage, preparing protein powder while producing chondroitin sulfate can increase the added value of production and reduce environmental pollution, but there is currently no report of such technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Soak fresh fish bones in water for 3 hours and place them in a cooking pot for 100 oC Cook for 30 minutes, remove the fish meat and oil, rinse with clean water, chop into pieces with a size of 1cm×1cm, and weigh them for later use. 50g of the treated cartilage was selected, and the aqueous solution with a pH value of 8.5 was added according to the ratio of material to liquid 1:2, and papain 5g / kg, neutral protease 5g / kg and compound enzyme (FM) 5g / kg were added respectively. 45 o C enzymolysis 6h. During the hydrolysis process, the pH of the solution should be constantly adjusted to 8.0-8.5. The complete degree of hydrolysis can be determined by adding 50g / L trichloroacetic acid dropwise to a little hydrolyzed solution to determine the degree of turbidity of the solution. Add dilute hydrochloric acid / phosphoric acid to the enzymolysis solution to adjust the pH value of the solution to 3.0, and heat up the temperature to 90 o The C solution was layered, continued to ...

Embodiment 2

[0037] Chondroitin Sulfate Ion Exchange Chromatography Purification Technology

[0038] Put the ion-exchange D001-cc resin in three 3×25cm chromatography columns, equilibrate the resin with acetic acid-sodium acetate buffer solution of pH 6.0, pH 5.0, and pH 4.0, until the effluent and eluent The pH is the same and equilibrium is complete. Add 20 mL of the chromatographic solution after enzymatic hydrolysis of chondroitin sulfate. After the chromatographic solution completely enters the resin, wash the column wall twice with the eluent, and then use pH4.0, pH5.0, and pH6.0 acetic acid- Elute with sodium acetate buffer at a flow rate of 60mL / h, collect the effluent, add ethanol to the above-mentioned ion exchange eluent until the ethanol content reaches 75%, chondroitin sulfate is precipitated, filter, wash with ethanol twice, and dry to obtain sulfuric acid Pure chondroitin.

Embodiment 3

[0040] Co-production technology of chondroitin sulfate and hydrolyzed collagen

[0041] Soak fresh fish bones in water for 3 hours and place them in a cooking pot for 100 oC Cook for 30 minutes, remove the fish meat and oil, rinse with clean water, chop into pieces with a size of 1cm×1cm, and weigh them for later use. Select 50g of the treated cartilage, add the aqueous solution with a pH value of 8.5 according to the ratio of material to liquid 1:2, add papain 5g / kg, neutral protease 10g / kg and compound enzyme (FM) 5g / kg respectively. 55 o C enzymatic hydrolysis for 5h. During the hydrolysis process, the pH of the solution should be constantly adjusted to 8.0-8.5. The complete degree of hydrolysis can be determined by adding 50g / L trichloroacetic acid dropwise to a little hydrolyzed solution to determine the degree of turbidity of the solution. Add dilute hydrochloric acid / phosphoric acid to the enzymolysis solution to adjust the pH value of the solution to 4.5, and heat ...

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PUM

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Abstract

The invention discloses a method for producing chondroitin sulfate with coproduction of hydrolyzed collagen by employing fish cartilage. The method comprises the following steps: 1, pretreatment; 2, enzymolysis; 3, heat treatment / filtration concentration; 4, resin adsorption; 5, chondroitin sulfate refining; and 6, protein recovery. The chondroitin sulfate and collagen are simultaneously hydrolyzed by employing the composite enzyme hydrolysis technology. The collagen released from the chondroitin sulfate and the collagen in the fish cartilage are partially hydrolyzed; and moreover, lots of protein pollutants produced by the traditional chondroitin sulfate are recycled, synchronous production of the chondroitin sulfate and hydrolyzed collagen is realized, the fish cartilage is comprehensively utilized, and the method achieves energy-saving, emission-reducing purposes and has obvious economic benefits and social benefits.

Description

technical field [0001] The invention relates to a method for producing chondroitin sulfate and co-producing hydrolyzed collagen from fish cartilage, belonging to the technical field of chemical industry. Background technique [0002] Chondroitin Sulfate (CS for short) is an important acidic mucopolysaccharide extracted from animal cartilage tissues such as laryngeal bone, nasal middle bone, and trachea. Chondroitin sulfate is widely distributed in tissues of humans and some animals such as cattle, sheep, pigs, fish, etc. Its main component is a mixture of isomers such as 4-chondroitin sulfate (CSA) and 6-chondroitin sulfate (CSC). Chondroitin sulfate is mainly used to treat cardiovascular and cerebrovascular diseases and joint diseases, and has the functions of lowering blood fat, antithrombotic, and antitumor. my country's chondroitin sulfate is exported to the United States and many European countries in large quantities, and it ranks third among the ten types of raw mate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C08B37/08
Inventor 曹荣军刘万顺孙杰杨庆利任宪君张秋艳武鑫刘少芳金海明姚秀芬台文静鲁涛
Owner QINGDAO BETTER BIO TECH
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