Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing chondroitin sulfate and co-producing hydrolyzed collagen from fish cartilage

A technology for hydrolyzing collagen and chondroitin sulfate, which is applied in the chemical industry to achieve comprehensive utilization, remarkable economic and social benefits

Inactive Publication Date: 2015-03-25
QINGDAO BETTER BIO TECH
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, considering the comprehensive utilization of fish cartilage, preparing protein powder while producing chondroitin sulfate can increase the added value of production and reduce environmental pollution, but there is currently no report of such technology

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Soak fresh fish bones in water for 3 hours and place them in a cooking pot for 100 oC Cook for 30 minutes, remove the fish meat and oil, rinse with clean water, chop into pieces with a size of 1cm×1cm, and weigh them for later use. 50g of the treated cartilage was selected, and the aqueous solution with a pH value of 8.5 was added according to the ratio of material to liquid 1:2, and papain 5g / kg, neutral protease 5g / kg and compound enzyme (FM) 5g / kg were added respectively. 45 o C enzymolysis 6h. During the hydrolysis process, the pH of the solution should be constantly adjusted to 8.0-8.5. The complete degree of hydrolysis can be determined by adding 50g / L trichloroacetic acid dropwise to a little hydrolyzed solution to determine the degree of turbidity of the solution. Add dilute hydrochloric acid / phosphoric acid to the enzymolysis solution to adjust the pH value of the solution to 3.0, and heat up the temperature to 90 oThe C solution was layered, continued to h...

Embodiment 2

[0038] Chondroitin Sulfate Ion Exchange Chromatography Purification Technology

[0039] Put the ion-exchange D001-cc resin in three 3×25cm chromatography columns, equilibrate the resin with acetic acid-sodium acetate buffer solution of pH 6.0, pH 5.0, and pH 4.0, until the effluent and eluent The pH is the same and equilibrium is complete. Add 20 mL of the chromatographic solution after enzymatic hydrolysis of chondroitin sulfate. After the chromatographic solution completely enters the resin, wash the column wall twice with the eluent, and then use pH4.0, pH5.0, and pH6.0 acetic acid- Elute with sodium acetate buffer at a flow rate of 60mL / h, collect the effluent, add ethanol to the above-mentioned ion exchange eluent until the ethanol content reaches 75%, chondroitin sulfate is precipitated, filter, wash with ethanol twice, and dry to obtain sulfuric acid Pure chondroitin.

Embodiment 3

[0041] Co-production technology of chondroitin sulfate and hydrolyzed collagen

[0042] Soak fresh fish bones in water for 3 hours and place them in a cooking pot for 100 oC Cook for 30 minutes, remove the fish meat and oil, rinse with clean water, chop into pieces with a size of 1cm×1cm, and weigh them for later use. Select 50g of the treated cartilage, add the aqueous solution with a pH value of 8.5 according to the ratio of material to liquid 1:2, add papain 5g / kg, neutral protease 10g / kg and compound enzyme (FM) 5g / kg respectively. 55 o C enzymatic hydrolysis for 5h. During the hydrolysis process, the pH of the solution should be constantly adjusted to 8.0-8.5. The complete degree of hydrolysis can be determined by adding 50g / L trichloroacetic acid dropwise to a little hydrolyzed solution to determine the degree of turbidity of the solution. Add dilute hydrochloric acid / phosphoric acid to the enzymolysis solution to adjust the pH value of the solution to 4.5, and heat ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for producing chondroitin sulfate and co-producing hydrolyzed collagen from fish cartilage. The method comprises the steps: (1) pretreating; (2) carrying out enzymolysis; (3) carrying out heat treatment / filtration concentration; (4) carrying out resin adsorption; (5) refining chondroitin sulfate; (6) recovering protein. According to the method, the synchronous hydrolysis of chondroitin sulfate and collagen is realized by adopting a compound enzyme hydrolysis technology; collagen in the fish cartilage is partially hydrolyzed while chondroitin sulfate is released; a great deal of protein contaminants resulting from traditional chondroitin sulfate production are recycled, the synchronous production of chondroitin sulfate and hydrolyzed collagen is realized, the comprehensive utilization of the fish cartilage is realized, and meanwhile, energy is saved and emission is reduced, so that economic and social benefits are remarkable.

Description

[0001] The present invention is a divisional application for an invention patent with the application number 201310262059.3, the application date is June 27, 2013, and the title of the invention is a method for producing chondroitin sulfate from fish cartilage and co-producing hydrolyzed collagen. technical field [0002] The invention relates to a method for producing chondroitin sulfate and co-producing hydrolyzed collagen from fish cartilage, belonging to the technical field of chemical industry. Background technique [0003] Chondroitin Sulfate (CS for short) is an important acidic mucopolysaccharide extracted from animal cartilage tissues such as laryngeal bone, nasal middle bone, and trachea. Chondroitin sulfate is widely distributed in tissues of humans and some animals such as cattle, sheep, pigs, fish, etc. Its main component is a mixture of isomers such as 4-chondroitin sulfate (CSA) and 6-chondroitin sulfate (CSC). Chondroitin sulfate is mainly used to treat cardi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P21/06C08B37/08
Inventor 曹荣军刘万顺孙杰杨庆利任宪君张秋艳武鑫刘少芳金海明姚秀芬台文静鲁涛
Owner QINGDAO BETTER BIO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com