Method for producing chondroitin sulfate and realizing clean co-production of protein peptides, bone oil and bone meal

A technology of chondroitin sulfate and protein peptide, which is applied in the chemical industry, can solve the problems of large ethanol consumption, long cycle, and difficult discharge of waste water to meet the standards, and achieve significant economic and social benefits

Inactive Publication Date: 2015-04-22
QINGDAO BETTER BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are three main methods for the production of chondroitin sulfate in China: salt hydrolysis, alkali hydrolysis and enzymatic hydrolysis. The method used more in China is to extract chondroitin sulfate by combining dilute alkali or concentrated alkali with enzymatic hydrolysis. The alkaline enzymatic hydrolysis production process has cumbersome steps and a long cycle, generally takes 3-5 days, and the product quality and yield are unstable. In the process of high-temperature cooking, alkaline extraction, enzymatic hydrolysis and decolorization, chondroitin sulfate is severely damaged. Low product yield, darkened solution color, large ethanol consumption, high production cost
[0004] At present, a large amount of animal protein, bone oil, bone residue and its degradation products produced in the production process of chondroitin sulfate are discharged without utilization. The untreated organic wastewater is black and smelly, and eutrophic, which brings serious pollution to the water environment.
At present, only a small number of relatively large-scale chondroitin sulfate production enterprises treat sewage, and generally adopt simple physical and chemical treatment as the main terminal treatment method, that is, a large amount of protein pollutants are all concentrated in the tail treatment, and the one-time investment is relatively large, and the operation cost High, the protein has not been utilized, and it is difficult to meet the discharge standard

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select 1,000 kg of chicken leg bones, crush them into pieces below 1 cm, put them into a vacuum tank, add 800 kg of distilled water, heat with steam, control the temperature in the tank to 65°C, and vacuum to 0.01 MPa, and heat for 2 hours. Put the feed liquid into the enzymatic hydrolysis tank to adjust the pH to 7.5; add 3 kg of trypsin, 1 kg of flavor protease and 4 kg of alkaline protease, keep the temperature at 35°C, and perform enzymatic hydrolysis for 4 hours. After enzymatic hydrolysis, use a disc-type three-phase centrifuge to centrifuge and separate, and the upper layer is separated to obtain 50 kg of bone oil; after the bone residue is pulverized, 760 kg of bone powder is obtained. Concentrate the centrifuged filtrate through a three-effect concentrator. During the concentration process, control the temperature of the first effect at 90°C, the second effect at 80°C, and the third effect at 65°C. When the volume is 50% of the original filtrate, stop the concen...

Embodiment 2

[0024] Select 1,000 kg of chicken ball bones, crush them into pieces below 1 cm, put them into a vacuum tank, add 900 kg of distilled water, heat with steam, control the temperature in the tank to 70°C, vacuum degree 0.02 MPa, and heat for 2.3 hours. Put the feed liquid into the enzymatic hydrolysis tank to adjust the pH to 8.0; add 4 kg of trypsin, 2 kg of flavor protease and 5 kg of alkaline protease, keep the temperature at 38°C, and perform enzymatic hydrolysis for 5 hours. After the enzymatic hydrolysis, the disc type three-phase centrifuge was used for centrifugal separation, and the upper layer was separated to obtain 55 kg of bone oil; after the bone residue was pulverized, 746 kg of bone powder was obtained. Concentrate the centrifuged filtrate through a three-effect concentrator. During the concentration process, control the temperature of the first effect at 93°C, the second effect at 83°C, and the third effect at 68°C. When the volume is 55% of the original filtrate...

Embodiment 3

[0026] Select 1,000 kg of duck breast bones, crush them into pieces below 1 cm, put them into a vacuum tank, add 950 kg of distilled water, heat with steam, control the temperature in the tank to 80°C, and the vacuum degree to 0.03 MPa, and heat for 2.5 hours. Put the feed liquid into the enzymatic hydrolysis tank to adjust the pH to 8.0; add 5 kg of trypsin, 3 kg of flavor protease and 7 kg of alkaline protease, keep the temperature at 40°C, and perform enzymatic hydrolysis for 6 hours. After enzymatic hydrolysis, centrifuge separation with a disc-type three-phase centrifuge, and 72 kg of bone oil is obtained from the upper layer separation; 735 kg of bone powder are obtained after the bone residue is pulverized. Concentrate the centrifuged filtrate through a three-effect concentrator. During the concentration process, control the temperature of the first effect at 95°C, the second effect at 85°C, and the third effect at 70°C. When the volume is 50% of the original filtrate, s...

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Abstract

The invention discloses a method for producing chondroitin sulfate and realizing clean co-production of protein peptides, bone oil and bone meal, which is characterized by comprising the following steps: (1) performing cartilage hydrolysis pretreatment; (2) performing enzymolysis; (3) performing centrifugal separation to obtain the bone oil and the bone meal; (4) concentrating; (5) adsorbing; (6) preparing the protein peptides; and (7) preparing the chondroitin sulfate. The invention adopts a composite enzyme hydrolysis technology and realizes synchronous hydrolysis of the chondroitin sulfate and the protein peptides. The chondroitin sulfate is released, and meanwhile, the protein peptides in the cartilage are hydrolyzed. A large amount of proteins and other pollutants in the traditional chondroitin sulfate production are recycled, thus realizing synchronous production of the chondroitin sulfate, the protein peptides, the bone oil and the bone meal, realizing comprehensive utilization of the cartilage, and simultaneously realizing energy saving and emission reduction and achieving remarkable economic benefits and social benefits.

Description

technical field [0001] The invention belongs to the technical field of chemical industry, and in particular relates to a method for cleaning and co-producing protein peptide, bone oil and bone meal with chondroitin sulfate. Background technique [0002] Chondroitin Sulfate (CS for short) is an important acidic mucopolysaccharide extracted from animal cartilage tissues such as laryngeal bone, nasal middle bone, and trachea. Chondroitin sulfate is widely distributed in tissues of humans and some animals such as cattle, sheep, pigs, fish, etc. Its main component is a mixture of isomers such as 4-chondroitin sulfate (CSA) and 6-chondroitin sulfate (CSC). Chondroitin sulfate is mainly used to treat cardiovascular and cerebrovascular diseases and joint diseases, and has the functions of lowering blood fat, antithrombotic, and antitumor. my country's chondroitin sulfate is exported to the United States and many European countries in large quantities, and it ranks third among the t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C08B37/08C11B1/10
Inventor 曹荣军任宪君张秋艳杨庆利孙杰刘少芳
Owner QINGDAO BETTER BIO TECH
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