Preparation method of giant salamander skin collagen peptide

Abstract

The invention relates to a preparation method of giant salamander skin collagen peptide. The method comprises the steps that degreasing and deodorization are conducted simultaneously on a giant salamander skin, black skin and impure protein are removed through alkali liquor, under the condition that the temperature ranges from 20 DEG C to 25 DEG C and the pressure ranges from 150 MPa to 300 MPa, pressure maintaining is conducted for 10-60 min, pH of the giant salamander skin processed through ultrahigh pressure is regulated to 8-10, alkaline protease with the weight of 2%-3% of the skin is added, at the temperature of 45-50 DEG C, continuous stirring and enzymolysis are conducted for 3-6 hours, the mixture is boiled for 1-2 min and cooled to 40 DEG C, pH of the mixture is regulated to 4-5, activated carbon with the mass of 1% of the mixture is added, and stirring and decoloration are conducted; under the condition that the rotating speed is 3000-4500 r / min, centrifugation is conducted on the processed mixture for 10-15 min, microfiltration through a filter membrane with the thickness of 0.45 micron, ultrafiltration through an ultrafiltration membrane with the molecular weight cut-off of 4-5 kD and nanofiltration through a nanofiltration membrane with the molecular weight cut-off of 2-3 kD are conducted on the mixture in sequence to obtain filter liquor, the filter liquor is concentrated to be one half of an original sample, and the concentrated liquor is freeze-dried and smashed to obtain giant salamander skin collagen powder. According to the preparation method of the giant salamander skin collagen peptide, the molecular weight of the collagen peptide is small, and over 90% of the molecular weight is smaller than 1000 Dalton.

Description

technical field [0001] The invention belongs to the field of giant salamander deep processing, and in particular relates to a preparation method of giant salamander skin collagen peptide. Background technique [0002] my country is the first country in the world to domesticate and breed giant salamanders and to use giant salamander products. It has a history of nearly 2,000 years to list giant salamanders as Chinese medicinal materials. Due to the high economic value of giant salamanders, my country's giant salamander industry has developed rapidly and vigorously. The giant salamander industry once maintained a leading edge in the international market. After decades of development, an industrial system has initially formed, especially in the giant salamander industry. Domestication and breeding, disease prevention and other aspects have reached the world's leading level. But since entering the 21st century, with my country's accession to WTO, the competitiveness of my countr...

AI Technical Summary

Problems solved by technology

Due to the difference in optimum temperature, optimum pH and isoelectric point of different proteases, all methods are not universal, especially for proteins with multiple isoelectric points such as collagen, choosing a suitable method to extract collagen peptides Difficult