Small molecule bovine bone collagen peptide and preparation method thereof

Abstract

The invention relates to a small molecule bovine bone collagen peptide and a preparation method thereof. The preparation method comprises the following steps in sequence: grinding, extraction separation, enzymolysis, composite filtration, concentration and drying, wherein in the enzymolysis process, bone collagenase is added to a bovine bone collagen extracting solution to carry out primary enzymolysis; after primary enzymolysis, the pH value of a primary enzymolysis solution is adjusted to be 6-7 with a mixed acid and then proline protease is added to carry out secondary enzymolysis for 2-4 hours; the addition of proline protease is 0.1-0.3% of the mass of bovine bone collagen. The small molecule bovine bone collagen peptide and the preparation method have the beneficial effects that according to the specificity of bovine bone collagen, proline protease and bone collagenase are adopted for the first time to carry out composite enzymolysis, so that the obtained product has strong practicability and the yield of the small molecule active peptide is as high as 90%.

Description

technical field [0001] The invention relates to the field of extraction of bioactive substances, in particular to a small molecule bovine collagen peptide and a preparation method thereof. Background technique [0002] my country is the largest meat producing country in the world. With the continuous increase in the number of slaughtering, a large number of by-products are also produced. At present, nearly 20 million tons of livestock and poultry bones can be produced every year. Taking bovine bone as an example, it accounts for about 12% to 20% of the bovine body mass, that is, 12 to 20 kg of bovine bone will be produced for every 100 kg of beef produced. In my country's bone processing industry, most of the animal bones are used to produce animal feed, such as the production of bone glue, bone oil, bone meal, etc., and their added value is very low, especially the research on the deep processing of bovine bone is less. The nutritional value of bone is rich, and the crude ...

AI Technical Summary

Problems solved by technology

[0004] In recent years, most of the relevant patents on enzymatic hydrolysis of bones use pepsin, alkaline protease, neutral protease, trypsin, etc., although some effects have been achieved, but because the main component of bone is collagen, its triple helical structure leads to the loss of collagen Proteins are difficult to decompose, and the molecular weight distribution of the products is wide and not concentrated, and the content of macromolecular peptides is high. Therefore, no real suitable enzymes for large-scale industrial production of bone degradation have been found so far.

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