Preparation method of good-biocompatibility, enzyme-responsive and antibacterial titanium material

A biocompatible, enzyme-responsive technology, used in tissue regeneration, surface reaction electrolytic coating, prosthesis, etc., can solve the problems of antibiotics not diffusing freely, low bacterial efficiency, etc., and achieve good biocompatibility and responsiveness. The effect of antibacterial ability, simple operation and strong controllability

Inactive Publication Date: 2018-07-24
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In a bacterial biofilm environment, antibiotics cannot diffuse fre

Method used

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  • Preparation method of good-biocompatibility, enzyme-responsive and antibacterial titanium material
  • Preparation method of good-biocompatibility, enzyme-responsive and antibacterial titanium material
  • Preparation method of good-biocompatibility, enzyme-responsive and antibacterial titanium material

Examples

Experimental program
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Effect test

Embodiment 1

[0067] A method for preparing a biocompatible enzyme-responsive antibacterial titanium material, characterized in that it comprises the following steps:

[0068] 1) Utilize the anodic oxidation method to construct the titanium dioxide nanotube that pipe diameter is 70nm as drug reservoir, and carry out the loading of antibiotic to it;

[0069] 1.1) Wash the titanium foil (10mm×10mm) with ethanol, acetone, ethanol and distilled water for 15 minutes respectively; after drying, perform electrochemical corrosion;

[0070] The temperature of the drying process is 60°C;

[0071] In the electrochemical corrosion process: the platinum foil is used as the cathode, and the titanium foil is used as the anode; the electrolytic solution is a water / glycerol mixture containing ammonium fluoride, the concentration of the ammonium fluoride is 0.27M, and the volume ratio of the water and glycerin is 1:1;

[0072] In the electrochemical corrosion process, the constant voltage is 20V, and the e...

experiment example 1

[0107] Release of CecB and degradation of multilayer films

[0108] In this experimental example, different concentrations of hyaluronidase (0.01, 0.05 and 0.5mg / ml) and Staphylococcus aureus and Escherichia coli with OD=0.5 were used to study the effect of hyaluronidase on the multilayer film on the surface of the material. Responsive release situation; specifically include the following steps:

[0109] 1) In order to verify the effect of hyaluronidase on drug release, 6 samples of each group (TNT@Van-LBL and TNT@Van-LBLc) were soaked in 500 μL PBS and PBS containing different concentrations of hyaluronidase (HAase- PBS) solution;

[0110] After incubation (37°C) for different times (2, 4, 8, 12, 24, 48, 72 h), 5 μL of leachate was taken from each group of samples to detect the release rate of Van.

[0111] In this experiment, the release amount of Van was measured by Nanodrop at a wavelength of 237nm, and the calculation formula was: Van (%)=(total release amount of Van) / (...

experiment example 2

[0123] Detection of osteoblast adhesion and differentiation on the material surface

[0124] When the osteoblasts grow to the third generation, 2×10 4 The density of one / well was inoculated on the surface of each titanium substrate, and cultured for 1 hour. Hochest33258 was used to stain the nucleus of osteoblasts for 5 minutes. The adhesion and differentiation of osteoblasts were observed under an inverted fluorescent microscope, and the number of cells was counted.

[0125] Figure 7 It is the cell adhesion situation (the blue color in the figure is the nucleus stained by H33258) of osteoblasts on the surface of different modified samples after culturing for 1 hour;

[0126] like Figure 7 As shown in (c), the surface of the Chi / HA multilayer film without catechol group modification is not conducive to the adhesion and differentiation of osteoblasts, and it is easy to aggregate into clumps, while Chi-c / HA-c The surface of the polyelectrolyte multilayer film promotes the a...

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Abstract

The invention discloses a preparation method of a good-biocompatibility, enzyme-responsive and antibacterial titanium material. The preparation method is characterized by comprising the following steps: firstly, constructing a uniform titanium dioxide nanotube on the surface of a titanium material by an anodic oxidation method, and using the uniform titanium dioxide nanotube as an antibiotic-loaded medicine storage reservoir; secondly, modifying hyaluronic acid and chitosan by using dopamine and dihydrocaffeic acid respectively to synthesize catechol-functionalized hyaluronic acid and chitosan, which are named as HA-c and Chi-c and used as a polyelectrolyte anion and a polyelectrolyte cation respectively; constructing a catechol-functionalized polyelectrolyte multilayer film on the surfaceof the antibiotic-loaded titanium dioxide nanotube by using the HA-c and the Chi-c by a layer-by-layer self-assembly technology. By the preparation method, biological coating with dual functions of promoting adhesion and differentiation of osteoblasts and achieving a bacterial responsive antibacterial property is constructed on the surface of the titanium material, and a titanium-based implant isendowed with good antibacterial and osteogenic properties.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a method for preparing a biocompatible enzyme-responsive antibacterial titanium material. Background technique [0002] Titanium and titanium alloys have excellent mechanical strength, biocompatibility and corrosion resistance, and are the most commonly used biomaterials in dental and orthopedic treatments. However, titanium-based surfaces are deficient in inducing specific cellular responses such as adhesion and stimulation due to their biological inertness, and thus have limited potential to achieve sufficient new bone formation for ideal osseointegration. [0003] In addition, bacterial infection after titanium implantation is still an important cause of implant failure. Once bacteria adhere to the titanium surface, they prevent the immune system from attacking through the formation of a biofilm. In a bacterial biofilm environment, antibiotics cannot diffuse freely into t...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/54A61L27/02A61L27/40A61L27/34C25F3/08C25D11/26C08B37/08
CPCA61L27/025A61L27/34A61L27/50A61L27/54A61L2300/252A61L2300/406A61L2430/02C08B37/003C08B37/0072C25D11/26C25F3/08C08L5/08
Inventor 蔡开勇袁璋黄骕洲熊海舟兰少雄和野
Owner CHONGQING UNIV
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