Fusion peptide capable of promoting osteoblast adhesion and osteogenic differentiation as well as preparation method and application of fusion peptide

A technology of cell adhesion and fusion peptide, applied in the fields of biology and medicine, can solve the problems of adhesion, proliferation, lack of osteogenic differentiation, etc., achieve good application prospects, promote adhesion, and promote the effect of proliferation

Active Publication Date: 2016-04-20
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the hydrogel structure can simulate the extracellular matrix (extracellular matrix, ECM) and its microenvironment, provide cells with a good in vitro growth environment, and is conducive to cell adhesion, proliferation and differentiation; Glue lacks the ability to promote osteogenic differentiation

Method used

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  • Fusion peptide capable of promoting osteoblast adhesion and osteogenic differentiation as well as preparation method and application of fusion peptide
  • Fusion peptide capable of promoting osteoblast adhesion and osteogenic differentiation as well as preparation method and application of fusion peptide
  • Fusion peptide capable of promoting osteoblast adhesion and osteogenic differentiation as well as preparation method and application of fusion peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Peptide Design

[0028] With RADA16-I peptide, the sequence is SEQ ID NO: 1 (AcN-RADARADARADARADA-CONH 2 ) as a basis, a section of osteostatin peptide (TRSAW) is directly coupled at its C-terminus by a peptide bond, and the amino acid sequence of the polypeptide (fusion peptide) is SEQ ID NO: 2 (RADARADARADARADAGGTRSAW), with a molecular weight of 2387.54; or at the C-terminus of the RADA16-I peptide The polypeptide (fusion peptide) amino acid sequence is SEQ ID NO: 3 (RADARADARADARADAGGWASRT) through the coupling of two amino acid residues GG and osteostatin peptide (TRSAW). The above peptides were all synthesized by Suzhou Qiangyao Biotechnology Co., Ltd.

Embodiment 2

[0029] Example 2 Peptides Promote Cell Adhesion, Proliferation, and Differentiation Performance

[0030] 1. Preparation of Self-Assembled Peptide Mother Solution

[0031] Add 1ml of sterile ultrapure water to the peptide powder (per 10mg aliquots), blow and mix with a pipette gun, and ultrasonically treat at room temperature for 30 minutes to remove the viscosity of the solution to obtain a 1% (m / v) peptide mother solution. Mix equal volumes of RADA16-I and RADA16-TRSAW mother liquors to obtain 1% (m / v) TRSAWmx mother liquors.

[0032] 2. Atomic force microscopy (AFM) to observe the fiber structure of self-assembled polypeptides

[0033] Dilute TRSAW, RADA16-I, RADA16-TRSAW, and TRSAWmx mother liquors with ultrapure water to obtain a 0.5‰ (m / v) dilution, and ultrasonically treat them at room temperature for 30 minutes with an ultrasonic cleaner. Take 5μL of different polypeptide dilutions and add them dropwise to a clean circular mica sheet. After standing for 15s, wash 3 ...

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Abstract

The invention provides a fusion peptide capable of promoting osteoblast adhesion and osteogenic differentiation as well as a preparation method and application of the fusion peptide. The fusion peptide comprises an RADA16-I peptide and an osteostatin (TRSAW) peptide coupled at the C end of the RADA16-I peptide sequence, wherein the amino acid sequence of the RADA16-I peptide is shown as SEQ ID NO:1 in the specification. The invention further provides a medicine composition comprising the fusion peptide. The fusion peptide provided by the invention not only can promote MC3T3-E1 osteocyte adhesion and proliferation, but also can promote osteogenic differentiation, and further has a good application prospect.

Description

technical field [0001] The present invention relates to the fields of biology and medicine, in particular, the present invention relates to a novel polypeptide capable of promoting the adhesion and osteogenic differentiation of osteogenesis-related cells and its application. Background technique [0002] Bone grafting is a common clinical treatment for repairing bone defects caused by spinal fusion, bone injury, and bone tumors. Autologous bone transplantation has always been regarded as the gold standard for bone transplantation, but its clinical application is limited due to limited materials, pain in the operation area, and increased risk of bleeding and infection. Scientists have been trying to find various alternative materials that are osteoconductive, osteogenic and osteoinductive. At present, a variety of synthetic bone substitute materials have been used clinically, such as hydroxyapatite, ceramic materials, etc., but synthetic bone substitute materials have disadv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C07K1/00A61K38/16A61P19/08
CPCA61K38/00C07K14/00C07K2319/00
Inventor 侯天勇陈贝克邢军超许建中
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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