Anti-aging nutritional compound for pets

Abstract

The invention relates to an anti-aging nutritional compound for pets, and belongs to the technical field of animal health. The anti-aging nutrient compound is prepared from the following components according to the daily average intake of each kilogram of body weight of a pet: 0.04 to 0.24 g of yeast powder, 0.0132 to 0.08 g of pork liver powder, 0.0132 to 0.08 g of wheat germ, 0.0068 to 0.04 g of malic acid, 0.0068 to 0.04 g of succinic acid, 0.0068 to 0.04 g of fumaric acid, 0.0068 to 0.04 g of taurine, 0.004 to 0.024 g of leucine, 0.004 to 0.024 g of isoleucine and 0.004 to 0.024 g of valine. All the components in the anti-aging nutrient compound designed by the invention have a synergistic effect through different mechanisms, jointly maintain the stability of cell energy metabolism and mitochondrial functions, and reduce oxidative stress and inflammatory response, so that the aging indication is improved, and the aging process is delayed.

Description

Technical Field

[0001] The invention relates to the technical field of animal health, and in particular to an anti-aging nutritional complex for pets. Background Art

[0002] Pets now occupy an important position in many families. As pets' lifespan increases, aging not only affects the quality of life of pets, but also brings emotional and financial burdens to pet owners. The health and quality of life of elderly pets are gradually receiving attention. Therefore, exploring effective anti-aging strategies is of great significance to improving the health and well-being of pets. Aging is a complex multifactorial process involving multiple aspects such as genes, environment, and lifestyle. Aging is closely related to biological processes such as oxidative stress, DNA damage, dysregulated cell metabolism, decreased autophagy function, and chronic inflammation.

[0003] At present, the mainstream anti-aging products on the market are mainly based on Chinese herbal medicine, plant essences or various amino acid compositions. For example, patent CN201910166881 provides a multi-effect compound supplement of negative ion fullerene that promotes youth and a preparation method. The disclosed supplement uses a large amount of amino acids and various plant essential oils. Although it can play a certain anti-aging role, its formula is complicated, the production process is cumbersome, and the cost is high, and it cannot be generally applied to all pets.

[0004] The tricarboxylic acid cycle (TCA cycle) is the core pathway of cellular energy metabolism, and its related substances have multiple biological functions in cells. In addition, the metabolites of the TCA cycle also participate in the antioxidant defense of cells, protecting cells from damage by oxidative stress, and play an important role in improving the anti-aging ability of animals. Summary of the invention

[0005] In order to solve the problems in the prior art, the present invention patent designs an anti-aging nutritional complex for pets to solve the problem that the existing anti-aging nutritional complex for pets is not ideal in application.

[0006] The technical solution adopted by the present invention is that the anti-aging nutritional complex includes 1 part of pork liver powder, 1 part of wheat germ, 3 parts of yeast powder, 0.5 parts of malic acid, 0.5 parts of succinic acid, 0.5 parts of fumaric acid, 0.5 parts of taurine and 1 part of amino acid composition.

[0007] Furthermore, the amino acid composition includes leucine, isoleucine and valine, and the mass ratio of the three is 1:1:1.

[0008] Furthermore, the anti-aging nutritional complex includes 0.0132-0.08 g of pork liver powder, 0.0132-0.08 g of wheat germ, 0.0132-0.08 g of yeast powder, 0.0068-0.04 g of malic acid, 0.0068-0.04 g of succinic acid, 0.0068-0.04 g of fumaric acid, 0.0068-0.04 g of taurine, 0.004-0.024 g of leucine, 0.004-0.024 g of isoleucine and 0.004-0.024 g of valine, based on the average daily intake per kilogram of body weight of the pet.

[0009] Furthermore, the anti-aging nutritional complex has an average daily intake of 0.1-0.7 g per kilogram of body weight of the pet.

[0010] The anti-aging nutritional complex provided by the present invention can be used in the form of an additive in the preparation of anti-aging food or health products for pets.

[0011] The anti-aging nutritional complex provided by the present invention can be used in the form of an additive in the preparation of antioxidant food or health products for pets.

[0012] The anti-aging nutritional complex provided by the present invention can be used in the form of an additive in the preparation of anti-inflammatory food or health products for pets.

[0013] The anti-aging nutritional complex provided by the present invention as described above can be added to the daily diet of pets in the form of supplements.

[0014] Furthermore, the anti-aging nutritional complex is applied as a supplement to the pet's diet in the form of freeze-dried, powdered, capsule or liquid.

[0015] Compared with the prior art, the anti-aging nutritional complex for pets designed in the present invention is an improvement in that the anti-aging nutritional complex provided by the present invention can enhance the TCA circulation flow of the animal body and resist the aging mechanism.

[0016] α-Ketoglutarate (AKG) provided by yeast powder is a key intermediate product of the TCA cycle. It can act as a cofactor for certain dioxygenases, participate in the demethylation process of DNA and histones, and downregulate the mammalian target of rapamycin signaling pathway to extend lifespan. Malic acid and succinic acid participate in the TCA cycle, help maintain the redox balance of mitochondria, and promote the efficient operation of the electron transport chain. Fumaric acid is converted into succinic acid in the TCA cycle, participates in regulating the metabolic pathways of cells, and participates in the adaptation of cells to hypoxic conditions by affecting the stability of hypoxia-inducible factors, enhances mitochondrial autophagy, and reduces the production of reactive oxygen species. Spermidine from wheat germ can affect cell autophagy and cell aging, and interacts with the intermediates of the TCA cycle to regulate the TCA cycle. Pyrroloquinoline quinone (PQQ) from pig liver powder participates in the mitochondrial electron transport chain as a coenzyme, improves the efficiency of energy production, and has antioxidant properties to protect mitochondria from oxidative damage. Leucine, isoleucine, and valine can be supplemented into the TCA cycle to maintain the balance of the TCA cycle, and play a role in muscle protein synthesis, promoting muscle growth and repair. Taurine protects mitochondria from damage, maintains their normal function, reduces DNA damage and relieves inflammation through antioxidant effects and regulation of calcium ion balance.

[0017] In the TCA cycle, PQQ, spermidine, AKG, malic acid, succinic acid, fumaric acid, taurine, leucine, isoleucine, and valine work through different mechanisms to promote cell energy metabolism and maintain mitochondrial function, and fight the aging process. The anti-aging complex is added to animal food and enters the pet's body through the oral route to regulate its metabolic state. It can also have a positive effect on the pet's appetite, digestion and absorption, and improve the pet's ability to fight aging. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 Results of NAD+ level detection in mouse heart tissue. Figure 2 These are the results of NAD+ level detection in mouse skeletal muscle tissue.

[0019] Figure 3 The average heart rate test result of the pet. Figure 4 Store test results for your pet's heart rate.

[0020] Figure 5 These are the results of the pet's 8-OH-dG (8-hydroxydeoxyguanine) level test.

[0021] Figure 6 This is the result of the pet's pro-inflammatory cytokine interleukin 6 (IL-6) level test. Figure 7The results of the pet's pro-inflammatory cytokine tumor necrosis factor (TNF-α) level test.

[0022] Figure 8 This is the test result of pet's weight.

[0023] Fig. 9 This is the test result of mouse survival time. DETAILED DESCRIPTION

[0024] The present invention is further described below in conjunction with the accompanying drawings and specific embodiments. The technical solutions in the embodiments of the present invention are clearly and completely described, and the described embodiments are only embodiments of a part of the present invention, not all of them. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] The present invention provides an anti-aging nutritional complex, wherein the composition ratio of each nutrient is: 1 part of pork liver powder, 1 part of wheat germ, 3 parts of yeast powder, 0.5 parts of malic acid, 0.5 parts of succinic acid, 0.5 parts of fumaric acid, 0.5 parts of taurine and 1 part of amino acid composition. The amino acid composition includes leucine, isoleucine and valine, and the mass ratio of the three is 1:1:1, and the yeast powder used has a high content of α-ketoglutaric acid.

[0026] Calculated based on the average daily intake per kilogram of body weight of the pet, the anti-aging nutritional complex includes 0.0132-0.08g of pork liver powder, 0.0132-0.08g of wheat germ, 0.0132-0.08g of yeast powder, 0.04-0.24g of malic acid, 0.0068-0.04g of succinic acid, 0.0068-0.04g of fumaric acid, 0.0068-0.04g of taurine, 0.004-0.024g of leucine, 0.004-0.024g of isoleucine and 0.004-0.024g of valine. Example

[0027] Effects of anti-aging nutritional complex on mitochondrial function Mitochondrial function plays an important role in maintaining cell growth and metabolism. NAD+, as a coenzyme of the TCA cycle, participates in the energy metabolism of mitochondria. Mitochondria use NAD+ to produce reducing equivalents, driving oxidative phosphorylation to produce ATP. The decline in NAD+ levels is an important sign of the aging process. C57BL / 6J mice were used to detect the effect of NAD+, a key component of the TCA cycle, on mitochondrial function. Ten 6-month-old young mice were taken as the young control group, and 20 18-month-old old mice were raised as the old group. The 18-month-old old mice were randomly divided into two groups. The old control group and the young control group were given normal mouse food, and the old experimental group was mixed with the anti-aging nutritional complex provided by the present invention in the same mouse food. After continuous feeding for 3 months. All mice were killed, and the mouse heart and skeletal muscle tissues were collected and immediately frozen in liquid nitrogen, and then the samples were collected and stored in a -20°C refrigerator. The NAD+ / NADH detection kit (from Biyuntian) was used according to the instructions.

[0028] like Figure 1 As shown, compared with the young control group, the NAD+ level in the heart tissue of the mice in the elderly control group decreased, while the NAD+ level in the heart tissue of the mice in the elderly experimental group increased after taking the anti-aging nutritional complex provided by the present invention. In addition, Figure 2 The same trend was shown in skeletal muscle tissue, indicating that as mice age, NAD+ levels decrease, and the anti-aging nutritional complex provided by the present invention helps to increase NAD+ levels, increase mitochondrial function, and thus increase TCA cycle flow, helping to restore heart and skeletal muscle function and help fight aging. Example

[0029] Effects of Anti-Aging Nutritional Complex on Cardiac Function in Pets Reduced heart function is one of the main signs of pet aging. Elderly pets are prone to heart failure, cardiomyopathy and other diseases, which affect the health and quality of life of pets. Take 18 healthy experimental dogs with no medical history, all weighing 9-12kg. 6 experimental dogs aged 3-5 years old were divided into a young control group, and the remaining 12 experimental dogs over 8 years old were divided into an elderly control group and an elderly experimental group. The control group was given ordinary dog ​​food that did not contain the anti-aging nutritional complex provided by the present invention, and the experimental group was given anti-aging dog food containing the anti-aging nutritional complex, and the other nutritional components were the same. The feeding cycle was 6 months, and at the end, a small animal heart rate monitor was used to measure the heart rate of each dog after running on a treadmill at a speed of 4-10km / h for 15 minutes, and the real-time heart rate of the dog was continuously monitored during running to calculate the heart rate reserve.

[0030] like Figure 3 As shown, compared with the young control group, the average heart rate of the dogs in the elderly control group decreased significantly, while the average heart rate of the elderly dogs increased significantly after taking the anti-aging nutritional complex provided by the present invention. Figure 4 It shows that the heart rate reserve of the dogs in the elderly control group is also lower than that of the dogs in the young control group. After taking the anti-aging nutritional complex provided by the present invention, the heart rate reserve of the dogs in the elderly experimental group is significantly improved, indicating that the anti-aging nutritional complex enhances the myocardial oxidation capacity of the dogs in the elderly experimental group, increases energy production, improves heart vitality, and maintains heart health. Example

[0031] Effects of anti-aging nutritional complex on oxidative stress in pets Oxidative stress is one of the causes of aging. Under oxidative stress conditions, excessive ROS can cause cell damage and accelerate aging. 8-OH-dG (8-hydroxydeoxyguanine) is a DNA damage marker, mainly caused by ROS. With age, the level of ROS in the body gradually increases, leading to the accumulation of DNA damage and increased 8-OH-dG levels. Take 18 healthy experimental dogs with no medical history, all weighing 9-12kg. Six 3-5 year old experimental dogs were used as the young control group, and the remaining 12 experimental dogs over 8 years old were evenly divided into the elderly control group and the elderly experimental group. The control group was given ordinary dog ​​food that did not contain the anti-aging nutritional complex provided by the present invention, and the experimental group was given anti-aging dog food containing the anti-aging nutritional complex, and the other nutritional components were the same. The feeding period was 6 months, and blood was collected from each dog at the end of the experiment. The whole blood was collected in an EDTA anticoagulant tube and centrifuged at 2500rpm for 20 min at 4°C to recover the plasma. The plasma was stored at -80°C. The DNA damage (8-OHdG) ELISA kit (source: Invitrogen) was used to detect the level of 8-OH-dG in the blood, and the detection method was performed according to the instruction manual.

[0032] like Figure 5 As shown, compared with the young control group, the 8-OH-dG level of the dogs in the elderly control group increased, while the 8-OH-dG level of the dogs in the elderly experimental group after taking the anti-aging nutritional complex provided by the present invention was significantly lower than that of the elderly control group, close to the young control group. It shows that the anti-aging nutritional complex reduces the DNA damage caused by ROS in the elderly experimental group dogs. About 90% of intracellular ROS are produced in the process of mitochondrial energy production, and mitochondrial dysfunction leads to excessive ROS. Therefore, this example shows that adding the anti-aging nutritional complex provided by the present invention can improve the oxidative stress level in the pet body, reduce DNA damage, and thus slow down aging by restoring mitochondrial function, increasing mitochondrial autophagy, and eliminating damaged and inefficient mitochondria that produce excessive ROS. Example

[0033] Effects of anti-aging nutritional complexes on pro-inflammatory factors in elderly pets Inflammation and aging are closely related. DNA damage and oxidative stress in the body can lead to increased inflammation levels and accelerate the aging process. 18 healthy experimental dogs with no medical history were taken, all weighing 9-12kg. Six 3-5-year-old experimental dogs were used as the young control group, and the remaining 12 experimental dogs over 8 years old were evenly divided into the elderly control group and the elderly experimental group. The control group was given ordinary dog ​​food without the anti-aging nutritional complex provided by the present invention, and the experimental group was given anti-aging dog food containing the anti-aging nutritional complex, and the other nutritional components were the same. The feeding period was 6 months, and blood was collected from each dog at the end of the experiment. The whole blood was collected in an EDTA anticoagulant tube and centrifuged at 2500 rpm for 20 min at 4°C to recover plasma. The plasma was stored at -80°C. The level of inflammatory factors was detected by enzyme-linked immunosorbent assay. According to the manufacturer's instructions, canine interleukin 6 (IL-6) ELISA kit and canine tumor necrosis factor α (TNF-α) ELISA kit (source Gilead) were used for detection according to the instructions.

[0034] like Figure 6 As shown, the IL-6 level of the experimental dogs in the young control group was lower, the IL-6 level of the dogs in the old control group was increased, and the IL-6 level of the dogs in the old experimental group given the anti-aging nutritional complex dog food provided by the present invention was significantly reduced. Figure 7 The same trend was observed in the experiment, the TNF-α level of the young control group experimental dogs was lower, the TNF-α level of the old control group dogs was increased, and the TNF-α level of the old experimental group dogs given the anti-aging nutritional complex dog food provided by the present invention was significantly reduced. IL-6 and TNF-α are two major pro-inflammatory cytokines that can provide information about systemic inflammatory responses and play an important role in aging. Activation of the mTOR pathway and excessive ROS can induce the release of inflammatory factors, and the key substances of the TCA cycle in the anti-aging nutritional complex provided by the present invention can mediate the downregulation of the mTOR pathway, and by restoring mitochondrial function, increase mitochondrial autophagy, and eliminate excess ROS. It can be seen that the anti-aging nutritional complex provided by the present invention can relieve chronic inflammation, improve immunity, and resist the aging process. Example

[0035] Effects of anti-aging nutritional complex on food intake and weight of elderly pets 18 healthy experimental dogs with no medical history were taken, and their weights were all between 9 and 12 kg. Six 3-5 year old experimental dogs were used as the young control group, and the remaining 12 experimental dogs over 8 years old were evenly divided into the elderly control group and the elderly experimental group. The control group was given ordinary dog ​​food without the anti-aging nutritional complex provided by the present invention, and the experimental group was given anti-aging dog food containing the anti-aging nutritional complex, and the other nutritional components were the same. The feeding period was 6 months, and each dog was tested for weight at the end of the experiment.

[0036] The results are as follows Figure 8As shown in the figure, the initial weight of the 18 healthy experimental dogs without a history of disease was 9-12kg. After the experiment, the weight of the elderly control group dogs was slightly lower than the initial weight, while the weight of the elderly experimental group dogs was relatively well maintained, higher than the elderly control group. This shows that the anti-aging nutritional complex can promote the absorption of nutrients in pets, maintain body weight, resist aging, and improve the quality of life of elderly pets. Example

[0037] Effects of anti-aging nutritional complex on lifespan of mice Meaningful anti-aging should not only improve the physiological state of elderly animals, but also prolong the survival time of animals. C57BL / 6J mice were used to detect the effects of key components of the TCA cycle on survival time, and the amount of component addition was investigated. 90 18-month-old mice were divided into six groups on average, with 15 mice in each group. The control group was given normal mouse food, and the experimental group was given the same mouse food with the anti-aging nutritional complex provided by the present invention added, wherein the amount of nutritional complex added was calculated as the average daily intake per kilogram of body weight of the mouse: 0.05g, 0.1g, 0.35g, 0.7g, and 1.4g, respectively. The two groups of mice were continuously fed and the survival status was observed and recorded until all mice died. The results are as follows Fig. 9 As shown, the mice in the control group began to die at the age of 21 months, and the mice in the 0.05g experimental group also began to die at the age of 21 months. However, the death time of the mice in the experimental groups with other added amounts was delayed, and the longest death occurred at the age of 24 months.

[0038] The median survival time of mice in each group was calculated. As shown in Table 1, the median survival time of mice in the control group was 29 months, while the median survival time of mice in the experimental group was extended to 30-34 months. Among them, the median survival time of mice in the experimental group under the condition of 0.1-0.7g increased by 17%. It can be seen that the anti-aging nutritional complex provided by the present invention prolongs the survival time of mice, further proving its anti-aging effect. The average daily intake of the anti-aging nutritional complex is preferably 0.1-0.7g per kilogram of body weight.

[0039] Table 1 Group control 0.05g / Kg 0.1g / Kg 0.35g / Kg 0.7g / Kg 1.4g / Kg Median survival time (months) 29 30 34 34 34 33 The above contents are only preferred embodiments of the present invention and cannot be used to limit the scope of implementation of the present invention. That is, any simple equivalent changes and modifications made according to the claims and description of the present invention are still within the scope of the present invention.

Claims

1. An anti-aging nutritional complex for pets, characterized in that: The anti-aging nutritional complex comprises 1 part of pork liver powder, 1 part of wheat germ, 3 parts of yeast powder, 0.5 parts of malic acid, 0.5 parts of succinic acid, 0.5 parts of fumaric acid, 0.5 parts of taurine and 1 part of amino acid composition.

2. An anti-aging nutritional complex for pets, characterized in that: The amino acid composition comprises leucine, isoleucine and valine, and the mass ratio of the three is 1:1:

1.

3. The anti-aging nutritional complex for pets according to claim 1, characterized in that: The anti-aging nutritional complex includes, based on the average daily intake per kilogram of body weight of the pet, 0.0132-0.08g of pork liver powder, 0.0132-0.08g of wheat germ, 0.04-0.24g of yeast powder, 0.0068-0.04g of malic acid, 0.0068-0.04g of succinic acid, 0.0068-0.04g of fumaric acid, 0.0068-0.04g of taurine, 0.004-0.024g of leucine, 0.004-0.024g of isoleucine and 0.004-0.024g of valine.

4. The anti-aging nutritional complex for pets according to claim 3, characterized in that: The average daily intake of the anti-aging nutritional complex is 0.1-0.7 g per kilogram of body weight of the pet.

5. The anti-aging nutritional complex for pets according to claim 4, characterized in that: The anti-aging nutritional complex is used in the form of an additive in the preparation of anti-aging food or health products for pets.

6. The anti-aging nutritional complex for pets according to claim 4, characterized in that: The anti-aging nutritional complex is used in the form of an additive in the preparation of antioxidant food or health products for pets.

7. The anti-aging nutritional complex for pets according to claim 4, characterized in that: The anti-aging nutritional complex is used in the form of an additive in the preparation of anti-inflammatory food or health products for pets.

8. The anti-aging nutritional complex for pets according to claim 4, characterized in that: The anti-aging nutritional complex is added to the pet's daily diet in the form of a supplement.

9. The anti-aging nutritional complex for pets according to claim 8, characterized in that: The anti-aging nutritional complex is applied as a supplement to the pet's diet in the form of freeze-dried, powdered, capsule or liquid.

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