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Fungus detection kit and application thereof

A technology for detecting kits and fungi, which is applied to the determination/inspection of microorganisms, microorganisms, methods based on microorganisms, etc., which can solve the problems of inconvenient operation and high cost, and achieve the effect of simple and convenient operation, low cost, and avoiding centrifugal operation

Active Publication Date: 2020-05-15
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the existing magnetic bead enrichment detection method, its cost is high, the operation is inconvenient, and many centrifugation steps are required, so it has not been widely used in clinical practice

Method used

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  • Fungus detection kit and application thereof
  • Fungus detection kit and application thereof
  • Fungus detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Disposable urine sampler, after taking a urine sample containing Candida, send it to the inspector within 2 hours. The solvent is 0.9% medical saline.

[0038] Detection process

[0039] 1) Dissolve 50mg of magnetic beads (purchased from Xiamen Primemag Biotechnology Co., Ltd.) in 1ml of equilibrium solution (containing 0.20g glucose, 0.10g mannose, 0.05g galactose and 0.05g maltose, histamine, sheath Amino alcohol, diprofos, benzothiazole, imidazole acrylic acid, glutamine each 0.0005g, 0.05g dextrin) and mix uniformly;

[0040] 2) Take 30ml of urine specimen and place it in a 50ml centrifuge tube, add 3ml of balance solution (containing 0.60g glucose, 0.30g mannose, 0.15g galactose and 0.15g maltose, histamine, sphingosine, triclophos, benzo Thiazole, imidazole, acrylic acid, glutamine each 0.0015g, 0.15g dextrin) mixed evenly;

[0041] 3) Mix the mixed solution obtained in step 2) and the magnetic beads obtained in step 1) at a volume ratio of 1500:1, and let stan...

Embodiment 2

[0047] Using the leucorrhea sample, the same detection as in Example 1 was carried out. The result is as image 3 As shown, after enrichment with magnetic beads, the number of colonies on the medium was significantly more than the number of colonies after culture in the sample before enrichment.

Embodiment 3

[0049] stability test

[0050] 1) Thermal stability test

[0051] The balance solution in Example 1 and the magnetic beads treated with the balance solution were placed in a light-proof water bath at 40°C for 3 days, no delamination was found with the naked eye, and no crystals were precipitated under the microscope, and then the test was carried out according to the operation steps of Example 1. No weakening of the enrichment effect was found.

[0052] 2) Cold stability test

[0053]The balance solution in Example 1 and the magnetic beads treated with the balance solution were placed in a -20°C refrigerator to avoid light for 1 month. During this period, they were repeatedly frozen and thawed several times. After taking them out, no delamination was found with the naked eye, and no crystals were precipitated under the microscope. The operation steps of Example 1 were tested, and it was found that the enrichment effect was not weakened.

[0054] 3) Normal temperature stabil...

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Abstract

The invention discloses a fungus detection kit. The fungus detection kit comprises magnetic beads and balance liquid, wherein the balance liquid comprises glucose, mannose, galactose and maltose, andthe ratio of the magnetic beads to the balance liquid is (50 mg-100 mg): 1 ml. The invention further discloses a fungus enrichment method. The fungus detection kit is used for enriching fungi quicklyand conveniently, and the cost is low; the enrichment effect is obvious; and repeated centrifugation is avoided.

Description

technical field [0001] The present application relates to the field of fungal detection, in particular to a fungal detection kit and its application. Background technique [0002] In recent years, due to the gradual increase in the number of tumor patients, organ transplantation, and immunodeficiency and immunocompromised patients, the incidence of fungal infections has greatly increased, especially deep fungal infections. Due to the lack of characteristic symptoms in the early stage of fungal infection, it is difficult to diagnose early clinically. Moreover, in the early stage of invasive fungal infection, the amount of fungus is small in the liquid samples collected in vivo, such as tissue fluid, blood, cerebrospinal fluid, pleural and peritoneal fluid, urine, and sputum, so it is difficult to detect fungi by direct microscopic examination of the samples. Although the method of molecular biology can detect the DNA of pathogenic fungi in the specimen more quickly, but this...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12N1/02C12R1/72C12R1/66C12R1/785
CPCC12Q1/04C12N1/02
Inventor 沈弢张浩吕琳婷
Owner PEKING UNIV
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