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Bacterial detection kit and application thereof

A bacterial detection and kit technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, and resistance to vector-borne diseases, etc., to achieve the effects of low cost, biosafety, and obvious enrichment effect.

Active Publication Date: 2020-06-19
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In response to these problems, in order to solve the problem of improving the adsorption efficiency of magnetic beads by special treatment under the existing special conditions

Method used

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  • Bacterial detection kit and application thereof
  • Bacterial detection kit and application thereof
  • Bacterial detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Disposable urine sampler, after taking a urine sample containing Staphylococcus aureus, send it to the inspector within 2 hours. The solvent is 0.9% medical saline.

[0037] Detection process

[0038] 1) Dissolve 50mg of magnetic beads (purchased from Xiamen Primemag Biotechnology Co., Ltd.) in 1ml of equilibrium solution (containing 0.20g glucose, 0.10g mannose, 0.05g galactose and 0.05g maltose, histamine, sheath Amino alcohol, diprofos, benzothiazole, imidazole acrylic acid, glutamine each 0.0005g, 0.05g dextrin) and mix uniformly;

[0039] 2) Take 30ml of urine specimen and place it in a 50ml centrifuge tube, add 3ml of balance solution (containing 0.60g glucose, 0.30g mannose, 0.15g galactose and 0.15g maltose, histamine, sphingosine, triclophos, benzo Thiazole, imidazole, acrylic acid, glutamine each 0.0015g, 0.15g dextrin) mixed evenly;

[0040] 3) Mix the mixed solution obtained in step 2) and the magnetic beads obtained in step 1) at a volume ratio of 1500:1...

Embodiment 2

[0043] Disposable urine sampler, after taking a urine sample containing Escherichia coli, send it to the inspector within 2 hours. The solvent is 0.9% medical saline.

[0044] Detection process

[0045] 1) Dissolve 50mg of magnetic beads in 1ml of equilibrium solution (containing 0.20g glucose, 0.10g mannose, 0.05g galactose and 0.05g maltose, histamine, sphingosine, diprofos, benzothiazole, imidazole, acrylic acid , glutamine each 0.0005g, 0.05g dextrin) and mix uniformly;

[0046] 2) Take 30ml of urine specimen and place it in a 50ml centrifuge tube, add 3ml of balance solution (containing 0.60g glucose, 0.30g mannose, 0.15g galactose and 0.15g maltose, histamine, sphingosine, triclophos, benzo Thiazole, imidazole, acrylic acid, glutamine each 0.0015g, 0.15g dextrin) mixed evenly;

[0047] 3) Mix the mixed solution obtained in step 2) and the magnetic beads obtained in step 1) at a volume ratio of 1500:1, and let stand for 10 minutes;

[0048] 4) Place the mixed solution...

Embodiment 3

[0052] stability test

[0053] 1) Thermal stability test

[0054] The balance solution in Example 1 and the magnetic beads treated with the balance solution were placed in a light-proof water bath at 40°C for 3 days, no delamination was found with the naked eye, and no crystals were precipitated under the microscope, and then the test was carried out according to the operation steps of Example 1. No weakening of the enrichment effect was found.

[0055] 2) Cold stability test

[0056] The balance solution in Example 1 and the magnetic beads treated with the balance solution were placed in a -20°C refrigerator to avoid light for 1 month. During this period, they were repeatedly frozen and thawed several times. After taking them out, no delamination was found with the naked eye, and no crystals were precipitated under the microscope. The operation steps of Example 1 were tested, and it was found that the enrichment effect was not weakened.

[0057] 3) Normal temperature stabi...

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Abstract

The invention discloses a bacterial detection kit. The detection kit comprises magnetic beads and a balancing solution, wherein the balancing solution comprises glucose, mannose, galactose and maltose, and the ratio of the magnetic beads to the balancing solution is (50mg-100mg):(1 ml). The invention also provides a method for enriching bacteria. When applied to bacteria enrichment, the bacteria detection kit is quick and convenient to use and low in cost; the enrichment effect is obvious; and repeated centrifugation is avoided.

Description

technical field [0001] The present application relates to the field of bacteria detection, in particular to a bacteria detection kit and its application. Background technique [0002] Clinical samples come from various sources, such as tissue fluid, blood, cerebrospinal fluid, pleural and ascites fluid, urine, sputum, etc. Every year, the rapid detection of bacteria is difficult due to the complex matrix of clinical samples, complex types of pathogens, and low content of pathogenic bacteria, resulting in missed diagnosis and poor prognosis of patients due to untimely treatment. Most of the detection of bacteria is through proliferation and culture to improve the detection sensitivity, but because some bacteria are difficult to culture, and it is easy to cause secondary pollution during the culture process, and it is very time-consuming (24-48h), which greatly reduces the detection efficiency. The timeliness of the results even missed the best treatment period. Although the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/04Y02A50/30
Inventor 沈弢张浩吕琳婷王誉雅
Owner PEKING UNIV
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