Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of fungus detection kit and its application

A technology for detecting kits and fungi, which is applied in the determination/inspection of microorganisms, microorganisms, methods based on microorganisms, etc., can solve the problems of high cost and inconvenient operation, and achieve low cost, simple and convenient operation, and obvious enrichment effect Effect

Active Publication Date: 2022-08-09
PEKING UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the existing magnetic bead enrichment detection method, its cost is high, the operation is inconvenient, and many centrifugation steps are required, so it has not been widely used in clinical practice

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of fungus detection kit and its application
  • A kind of fungus detection kit and its application
  • A kind of fungus detection kit and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Disposable urine sampler, after taking a urine sample containing Candida, send it to the inspector within 2 hours. The solvent is 0.9% medical saline.

[0038] Detection process

[0039] 1) Dissolve 50mg of magnetic beads (purchased from Xiamen Primaige Biotechnology Co., Ltd.) in 1ml of equilibrium solution (containing 0.20g glucose, 0.10g mannose, 0.05g galactose and 0.05g maltose, histamine, sphingomyelin 0.0005g each of amino alcohol, dichlorfenuron, benzothiazole, imidazole acrylic acid, and glutamine, and 0.05g dextrin) and mix them evenly;

[0040] 2) Take 30ml of urine sample and place it in a 50ml centrifuge tube, add 3ml of balance solution (containing 0.60g of glucose, 0.30g of mannose, 0.15g of galactose and 0.15g of maltose, histamine, sphingosine, diazophos, benzoin Thiazole, imidazole acrylic acid, glutamine each 0.0015g, 0.15g dextrin) mixed evenly;

[0041] 3) Mix the mixed solution obtained in step 2) and the magnetic beads obtained in step 1) in a ...

Embodiment 2

[0047] Using the leucorrhea sample, the same detection as in Example 1 was performed. The result is as image 3 As shown, after enrichment with magnetic beads, the number of colonies on the medium was significantly higher than that after the samples were cultured before enrichment.

Embodiment 3

[0049] Stability test

[0050] 1) Thermal stability test

[0051] The balance solution in Example 1 and the magnetic beads treated with the balance solution were placed in a water bath at 40°C in the dark for 3 days, no delamination was found with the naked eye, and no crystals were precipitated under the microscope, and then the test was carried out according to the operation steps of Example 1, It was found that the enrichment effect was not diminished.

[0052] 2) Cold stability test

[0053]The balance solution in Example 1 and the magnetic beads treated with the balance solution were placed in a -20°C refrigerator to avoid light for 1 month, during which time the freeze-thaw was repeated several times, taken out, no delamination was found with the naked eye, and no crystals were precipitated under the microscope. The operation steps of Example 1 were tested, and it was found that the enrichment effect was not weakened.

[0054] 3) Normal temperature stability test

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The application discloses a fungal detection kit, comprising magnetic beads and a balance solution, wherein the balance solution includes glucose, mannose, galactose and maltose, and the ratio of the magnetic beads to the balance solution is 50mg-100mg: 1ml. The present application also discloses a method for enriching fungi. Using the fungal detection kit of the present application to enrich fungi is fast, convenient, and low in cost; the enrichment effect is obvious; repeated centrifugation is avoided.

Description

technical field [0001] The present application relates to the field of fungal detection, in particular to a fungal detection kit and application thereof. Background technique [0002] In recent years, the incidence of fungal infections, especially deep fungal infections, has greatly increased due to the gradual increase in the number of tumor patients, organ transplants, and immunodeficiency and immunocompromised patients. Due to the lack of characteristic symptoms in the early stage of fungal infection, early diagnosis is difficult clinically. Moreover, in the early stage of invasive fungal infection, the amount of fungi in the liquid samples collected in the body, such as tissue fluid, blood, cerebrospinal fluid, pleural and ascites, urine and sputum, is very small, so it is difficult to detect fungi by direct microscopic examination of the specimen. Although the use of molecular biology methods can rapidly detect the DNA of pathogenic bacteria and fungi in specimens, thi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12N1/02C12R1/72C12R1/66C12R1/785
CPCC12Q1/04C12N1/02
Inventor 沈弢张浩吕琳婷
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com